Quantitative urine amino acid analysis using liquid chromatography tandem mass spectrometry and aTRAQ® reagents

Held, Patrice K.; White, L. E.; Pasquali, Marzia

doi:10.1016/j.jchromb.2011.07.030

Cited by 71 publications

(61 citation statements)

References 15 publications

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“…Therefore, all further results have been back calculated against this external calibration curve, which was reanalyzed twice before each run. Additionally, opting for this quantification way helps to decrease the batch-to-batch variability (Held et al 2011). …”

Section: Internal Versus External Calibrationmentioning

confidence: 99%

“…Nowadays, identification and quantification of amino acids by mass spectrometry are rather uncommon in clinical laboratories. Only a few methods have already been developed and validated and are based either on native (Piraud et al 2003(Piraud et al , 2005Waterval et al 2009) or on derivatized (Dettmer et al 2012;Dietzen et al 2008;Harder et al 2011;Held et al 2011) metabolites identification.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Physiological Amino Acids Profiling by Tandem Mass Spectrometry

2013

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Background: Nowadays, the most conventional method to quantify physiological amino acids consists in ion exchange chromatography (IEC) followed by post-column ninhydrin derivatization and UV detection at two wavelengths. Unfortunately, the technique presents some drawbacks such as long run time, large sample volume, and specific costs associated to the maintenance of a dedicated instrument. Therefore, we aimed to switch towards a mass spectrometry approach.Methods: We have tested the aTRAQ kit for Amino Acid Analysis of Physiological Fluids (AB Sciex), affording the selective quantification of about 40 amino acids, and present here the results of our assessments.Results: Outlined accuracy profiles for each amino acid demonstrated very reliable data. A good linearity was observed from 1 to 1,000 mmol/L. Results comparison with IEC showed a right concordance. Reference intervals established were very similar to those obtained by IEC and patients suffering from inborn error of metabolism have been readily identified.

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Section: Internal Versus External Calibrationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of Physiological Amino Acids Profiling by Tandem Mass Spectrometry

2013

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“…This method allows detection of both ASA and ASA anhydrides without any need for further sample preparation, even at very low concentrations which would be undetectable by traditional IEC. Additionally, we reviewed 5 years (2009-13) of IEC results obtained by our clinical laboratory to evaluate the need for increased sensitivity and specificity for this analyte.Briefly, 45 amino acids and related compounds were labeled using the aTraq™ reagents from AB Sciex [12][13][14]; the chromatographic separation was obtained using ABSciex amino acid analysis column (C18, 4.6 × 150 mm, 5 μm); the LC-MS/MS system was an API 4000 tandem Clinica Chimica Acta 442 (2015) 73-74 http://dx. …”

mentioning

confidence: 99%

“…Briefly, 45 amino acids and related compounds were labeled using the aTraq™ reagents from AB Sciex [12][13][14]; the chromatographic separation was obtained using ABSciex amino acid analysis column (C18, 4.6 × 150 mm, 5 μm); the LC-MS/MS system was an API 4000 tandem Clinica Chimica Acta 442 (2015) 73-74 http://dx.…”

mentioning

confidence: 99%

“…Amino acid quantitation was obtained using specific internal standards, pre-labeled as part of the aTraq kit, together with a 6-point external calibration curve for each amino acid [13]. The assay analytical performance was similar to previously published data [13,14]. The new method shows a good correlation with the IEC method (Biochrom 20 plus or Biochrom 30 Amino Acid Analyzers [11]) (slopes between 0.93 and 1.04, y-intercepts between − 7.0 and +7.0, R 2 N 0.96).…”

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confidence: 99%

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Quantitative amino acid analysis by liquid chromatography–tandem mass spectrometry: Implications for the diagnosis of argininosuccinic aciduria

Biase

Liu

Yuzyuk

et al. 2015

Clinica Chimica Acta

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A simple LC‐MS/MS method for quantitative analysis of underivatized neurotransmitters in rats urine: assay development, validation and application in the CUMS rat model

Zhai

Chen

Zhu

et al. 2015

Biomedical Chromatography

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Many amino acid neurotransmitters in urine are associated with chronic stress as well as major depressive disorders. To better understand depression, an analytical LC-MS/MS method for the simultaneous determination of 11 underivatized neurotransmitters (4-aminohippurate, 5-HIAA, glutamate, glutamine, hippurate, pimelate, proline, tryptophan, tyramine, tyrosine and valine) in a single analytical run was developed. The advantage of this method is the simple preparation in that there is no need to deconjugate the urine samples. The quantification range was 25-12,800 ng mL(-1) with >85.8% recovery for all analytes. The nocturnal urine concentrations of the 11 neurotransmitters in chronic unpredictable mild stress (CUMS) model rats and control group (n = 12) were analyzed. A series of significant changes in urinary excretion of neurotransmitters could be detected: the urinary glutamate, glutamine, hippurate and tyramine concentrations were significantly lower in the CUMS group. In addition, the urinary concentrations of tryptophan as well as tyrosine were significantly higher in chronically stressed rats. This method allows the assessment of the neurotransmitters associated with CUMS in rat urine in a single analytical run, making it suitable for implementation as a routine technique in depression research.

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Quantitative urine amino acid analysis using liquid chromatography tandem mass spectrometry and aTRAQ® reagents

Cited by 71 publications

References 15 publications

Evaluation of Physiological Amino Acids Profiling by Tandem Mass Spectrometry

Evaluation of Physiological Amino Acids Profiling by Tandem Mass Spectrometry

Quantitative amino acid analysis by liquid chromatography–tandem mass spectrometry: Implications for the diagnosis of argininosuccinic aciduria

A simple LC‐MS/MS method for quantitative analysis of underivatized neurotransmitters in rats urine: assay development, validation and application in the CUMS rat model

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