Quantitative variation of the common acute lymphoblastic leukemia antigen (gp100) on leukemic marrow blasts.

Cancer-associated mutations at the INK4a locus cancel cell cycle arrest by p16 INK4a but not by the alternative reading frame protein p19 ARF

“…After 3-6 hr, 10 ml of fresh medium was added, and the medium was changed the following day. Cells were analyzed by dual-color flow cytometry 48 hr after transfection or infection to determine the DNA content of gated CD8-positive and CD8-negative cells (22).…”

Inhibition of Cell Proliferation by the Mad1 Transcriptional Repressor

“…Approximately 75,000 cells from each sample were analyzed on a FACScan flow cytometer (Becton Dickinson, San Jose, Calif.), collecting forward-and sidescatter green fluorescence from fluorescein isothiocyanate-labeled CD8 molecules and red fluorescence from propidium iodide-stained DNA. DNA content histograms of cells expressing transfected genes were obtained by using electronic gating to select those cells with CD8-fluorescein isothiocyanate fluorescence greater than the background level (17,23). The percentages of cells within the G 1 , S, and G 2 /M phases of the cell cycle were determined by analysis of the single-parameter DNA histogram with the computer program ModFit (Verity Software House, Topsham, Maine).…”

Immunophenotype of normal and leukemic bone marrow B-precursors in a Brazilian population. A comparative analysis by quantitative fluorescence cytometry

“…Previous studies have demonstrated that differences in the intensity of B-antigen expression are related to B-maturation and to cell proliferation (26)(27)(28)(29). Accordingly, normal and leukemic cells expressing higher CD10 intensities are more immature and have higher proliferating rates than CD10 weak cells (26)(27)(28)(29). Although the intensity of CD10 expression may be related to cell cycle and maturation, it is not an independent prognostic factor in ALL (30,31).…”