Quantum dot immunoassays in renewable surface column and 96-well plate formats for the fluorescence detection of botulinum neurotoxin using high-affinity antibodies

Warner, Marvin G.; Grate, Jay W.; Tyler, Abby; Ozanich, Richard M.; Miller, Keith; Lou, Jianlong; Marks, James D.; Bruckner-Lea, Cynthia J.

doi:10.1016/j.bios.2009.06.031

Cited by 65 publications

(31 citation statements)

References 58 publications

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“…In addition to the microcolumn, a syringe pump, a holding coil, and a multiposition valve are needed to mix the reagents and to aspirate the beads into the flow system, and finally to the column for washes and measurement. The technology has potential to be fully automated [29,30].…”

Section: Microfluidics and Lab-on-a-chip Applicationsmentioning

confidence: 99%

Simple and inexpensive immunoassay-based diagnostic tests

Päkkilä

Soukka

2011

Bioanal Rev

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Simple and inexpensive yet sensitive and robust diagnostic tests are critically needed for resource-poor settings to enable timely diagnosis and effective use of limited health care resources. Current tests are often too expensive, too slow, or have compromised clinical performance, and they often require health care professional to perform the test. In addition, most assays are not intended to be used in extreme environmental conditions, but their performance may be affected by high temperatures and humidity often encountered in resource-poor settings. This review provides an overview of current immunoassay technologies and their advantages and limitations with respect to their feasibility to resourcepoor settings. Future trends of immunoassay development for decentralized testing are also discussed. Homogeneous assays as such are out of the scope of this article because they are generally not yet sensitive enough or otherwise less feasible for inexpensive rapid diagnostic tests.

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Section: Microfluidics and Lab-on-a-chip Applicationsmentioning

confidence: 99%

Simple and inexpensive immunoassay-based diagnostic tests

Päkkilä

Soukka

2011

Bioanal Rev

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“…Várias toxinas bacterianas de origem proteica, incluindo a toxina botulínica sorotipo A, foram detectadas, em níveis picomolar(10 -12 M), utilizando anticorpos luminescentes marcados com pontos quânticos, o que seria útil em aplicações anti-bioterrorismo [47] . A literatura relata imunossensores elétricos desenvolvidos para detectar a presença de aflatoxina-B17 em amostras de leite contaminado, até uma concentração de 0,01 ng/mL [48] .…”

Section: Embalagens Inteligentes: Incorporadas Com Nanosensores Para unclassified

Aplicação de nanotecnologia em embalagens de alimentos

et al. 2015

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AesumoA nanotecnologia tem grande potencial de aplicação na indústria de alimentos. No desenvolvimento de embalagens, pode proporcionar diferentes alternativas, tais como o desenvolvimento de nanopartículas, nanodispersões, nanolaminados, e nanotubos, que, associados aos polímeros, podem fornecer diversas funções. Por exemplo, pela incorporação de nanopartículas com propriedades antimicrobianas; nanosensores capazes de detectar produtos químicos, agentes patogênicos e toxinas em alimentos; nanopartículas bioativas capazes de manter os compostos em condições ideais, até a sua migração para o produto alimentício e nanocompósitos, que melhorem as propriedades de flexibilidade, barreira a gases e umidade e quanto à absorção de irradiação UV dos materiais aos quais são incorporados, assim como a estabilidade frente à temperatura. Este artigo relata algumas aplicações da nanotecnologia em embalagens de alimentos, bem como questões sobre toxicidade e regulamentação relacionadas à possibilidade de migração das nanopartículas para os alimentos; razão pela qual o setor alimentício tem sido cauteloso com a utilização desses materiais. Palavras-chave: toxicidade, regulamentação, nanopartículas, embalagem ativa, embalagem inteligente. AbstractNanotechnology has great application potential in the food industry. In packaging development, it can provide several alternatives, such as the formation of nanoparticles, nanodispersions, nanolayered and nanotubes, which, associated with polymers, can provide multiple functions. For example, by embedding nanoparticles with antimicrobial properties; nanosensors capable of detecting chemicals products, pathogens and toxins in food; bioactive nanoparticles capable of maintaining compounds at optimal conditions until its migration to the food product and nanocomposites, which improve the properties of flexibility, gas and humidity barrier and UV irradiation absorption of the materials to which are incorporated, as well as stability against temperature. This article reports some applications of nanotechnology in food packaging, as well as questions regarding toxicity and regulations related to the migration of nanoparticles to the food products; reason why the food industry has been cautious with the utilization of these materials.Keywords: toxicity, regulation, nanoparticles, active packaging, intelligent packaging. IntroduçãoAtualmente, grande parte das embalagens utilizadas no setor alimentício é de plástico, produzidas com base em polímeros oriundos do petróleo. Em geral, na comparação com outros materiais como papel, madeira, metais, vidros e cerâmicas, as embalagens plásticas destacam-se pelas vantagens que apresentam quanto às características físico-mecânicas como peso, flexibilidade, resistência mecânica, bem como outras características físico-químicas e biológicas, vinculada à qualidade, saúde e segurança, algumas das quais serão abordadas mais adiante. Em conjunto, essas características além de proporcionarem vantagens significativas no processamento de embalagens e adição de valo...

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“…Some electrochemical devices which are imunosensors along with cerium oxide nanoparticles and chitosan nanocomposites using nanowire based transistors and carbon nanotubes which detect several toxins like staphylococcal enterotoxin [21] and cholera toxin [22]. These detector systems at nano level have an advantage that they are able to detect impurities, pollutants and toxic chemicals in very low quantity even at nanolevel [23].…”

Section: Detection Of Chemicalsmentioning

confidence: 99%

Nanotechnology and Food. Is Nanotechnology for Food Applications Safe? : A Review

Khan¹

2015

NSTOA

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Nanoscience & Technology: Open AccessOpen Access Review Article nanotechnology arising a new emerging discipline called food nanotechnology. Literally Food and Drug Administration USA (FDA) gives the definition of food nanotechnology in a sense that the nanomaterials used in this case can have size up to 1000 nm because there has been some evidences that even at larger size the particles have same behavior at smaller scale [3].The technology provides its services in almost all sectors of food from production to marketing. In agriculture sector there are several applications available like nano-tech based pesticides and fertilizers with effective impact on plant growth, targeted genetic engineering and molecular farming with the help of nano-vectors which is hoping to take the place of viral vectors [4]. In food processing various industries are also taking leads and making the food with better quality and good nutritive value by utilizing the knowledge of nanotechnology and applying it in food sciences. For example, high impermeable packaging nanomaterials are used for protection of food from UV radiations and providing more strength to keep the food protected from outer environment, thus increasing their shelf life. Beside, nanosensors are also used for the detection of chemicals, gases and pathogens which can represent the spoilage and its rate. In modern terminology a word is given to such type of packaging which is known as smart packaging. Nutritive supplements using polymeric nano-coating with greater bioavailability are also introduced to complete the nutritional requirements. And it is a fact that the most participating research in food nanotechnology is in food processing section in which many researchers and industries are increasing their research in food safety with the help of nanotechnology. Some studies suggested that people are not accepting the direct involvement of nano-particles in food [5] due to some risk factors. So it is needed to provide some safety measurements by creating methods that avoid such direct involvement to reduce the risk and satisfy the community. Organization of Food Structure at Nano-LevelNanoparticles are not always synthetic but nature provide us a view of thinking how these particles are present in nature in various forms as in food, which is a combination of nano versus micro-particles. These molecules make an ordered organization AbstractFood nanotechnology is a combined discipline of food science and nanotechnology. It provides many applications almost in all areas of food technology. This article reviews the main focus in food nanotechnology research of food safety in various forms in which nano-biosensors and antimicrobials nano-agents are the works of interest. These tools are helpful in providing food security and together with nano-coating materials. It makes the base of smart packaging. It is now being estimated from the review that these nanoagents have great contribution in food safety but the health hazard risks due to accumulation of nanomaterials in food s...

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Quantum dot immunoassays in renewable surface column and 96-well plate formats for the fluorescence detection of botulinum neurotoxin using high-affinity antibodies

Cited by 65 publications

References 58 publications

Simple and inexpensive immunoassay-based diagnostic tests

Simple and inexpensive immunoassay-based diagnostic tests

Aplicação de nanotecnologia em embalagens de alimentos

Nanotechnology and Food. Is Nanotechnology for Food Applications Safe? : A Review

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