Quantum-DoT submicrobead-based immunochromatographic assay for quantitative and sensitive detection of zearalenone

Evaluation and Optimization of Three Different Immunoassays for Rapid Detection Zearalenone in Fodders

“…Despite the practical use of ICA does not require sequential addition of reagents, it is not suitable in some cases when the quantification of an analyte is concerned. Recent study has reported quantum-dot sub microbeads-based ICA exhibited a good dynamic linear detection for ZEN over the detection range of 0.125 to 10 ng/mL with a IC 50 of 1.017 ± 0.09 ng/mL (n = 3) (Duan et al 2015), suggesting that the technological breakthrough based on traditional detection technology and electronic science might be the trend for the development of detection kits with high sensitivity, accurate quantification and suitable for high throughput screening.…”

Immunochromatographic assay for quantitative and sensitive detection of hepatitis B virus surface antigen using highly luminescent quantum dot-beads

“…Moreover, the immunological kinetics of the QB-ICA was analyzed to elaborate the effect of the interpretation time on the stability of HBsAg quantitative analysis. The dynamic curves were indicated by plotting the values of FI T , FI C and the ratio of FI T /FI C against immunoreaction time according to our previously reported method [23]. Briefly, a positive serum sample containing 0.3 ng mL À 1 of HBsAg was mixed with QB probe and then added into the sample well, and the strip was scanned with a commercial fluorescent reader to record the values of FI T , FI C and the ratio of FI T /FI C every 30 s for a total of 45 min immunoreaction time.…”

An on-site, ultra-sensitive, quantitative sensing method for the determination of total aflatoxin in peanut and rice based on quantum dot nanobeads strip

“…It can also achieve a sensitive, low-cost determination for AFT, thereby guaranteeing the potential on-site application. Typically, the gold nanoparticle has been substantiated as an effective reporter for colorimetric detection, allowing the qualitative determination for mycotoxin with relatively high concentrations [10,11,12,13].Furthermore, to meet the requirements of detection of sensitive mycotoxins such as AFT, some quantitative test strip methods have been developed recently using various reporters, such as liposome-encapsulated dyes [14], magnetic nanogold microspheres [15],time-resolved fluorescence [16,17,18], up-converting phosphor [19], fluorescent microspheres [20], and quantum dots (QDs) [21,22,23].Owing to the intrinsically high sensitivity, the fluorescence-based strip could qualify application in food safety [9].The organic fluorescence could be hampered from the extensive application due to their inherent photobleaching, thus lowering the sensitivity. QDs have been proven as one of the optimum reporters.…”

“…However, the single QD could not be sensitive due to its longstanding issue of chemical and colloidal instability in physiological environments after the phase-transfer procedure [25,26].In order to address these problems, doping or encapsulating the beads as a signal amplification labelto gain both good chemical and colloidal stability was effective. For example, Li et al fabricated a test strip, utilizingquantum dot nanobeads (QDNBs) as probes for prostate specific antigen analysis, finding a LOD (limit of detection) of 12-fold lower than the result obtained by single QDs as fluorescent labels [27].Although there were similar reporters for ZEA or AFB 1 determination in agro-products [23,28], there remained great challenge for the AFT sensing via the encapsulated or doped QD nanobeads. The reason could be the inevitable matrix effect from complicated agro-product samples which would significantly arouse the background noise and then reduce the sensitivity.…”