2017
DOI: 10.1002/bio.3309
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Quantum dots‐based fluorescence resonance energy transfer biosensor for monitoring cell apoptosis

Abstract: The development of advanced methods for accurately monitoring cell apoptosis has extensive significance in the diagnostic and pharmaceutical fields. In this study, we developed a rapid, sensitive and selective approach for the detection of cell apoptosis by combining the site-specific recognition and cleavage of the DEVD-peptide with quantum dots (QDs)-based fluorescence resonance energy transfer (FRET). Firstly, biotin-peptide was conjugated on the surface of AuNPs to form AuNPs-pep through the formation of a… Show more

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Cited by 8 publications
(6 citation statements)
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“…Hence the detection of tumor postoperative recurrence is vital to patients. FRET is a powerful technique for the application of biological assays 40 . There were some reports about FRET technology combing CdTe QDs with AuNRs 41 , 42 .…”
Section: Resultsmentioning
confidence: 99%
“…Hence the detection of tumor postoperative recurrence is vital to patients. FRET is a powerful technique for the application of biological assays 40 . There were some reports about FRET technology combing CdTe QDs with AuNRs 41 , 42 .…”
Section: Resultsmentioning
confidence: 99%
“…Gu et al [9] developed a QDS-based FRET biosensor for monitoring cell apoptosis. The mechanism has been shown in Figure 1.…”
Section: Applications Of Fluorescent Biosensorsmentioning
confidence: 99%
“…Figure 1 The mechanism of QDs-based fluorescent biosensor for cell apoptosis analysis [9]. Wang et al [10] used hydrothermal method to synthesize sulfur and nitrogen co-doped carbon quantum dots as fluorescent probes to detect uric acid.…”
Section: Applications Of Fluorescent Biosensorsmentioning
confidence: 99%
“…Gu et al used the caspase-3 selective peptide (sequence: GDGDEVDGC) as a bridge between QDs and gold nanoparticles (AuNPs), constructing a new type "turn on"biosensor for monitoring cell apoptosis through caspase-3 content. 19 In addition to the PLGVRG sequence mentioned above, peptide SGAVRWLLTA is also a specific sequence toward MMP-2. MMP-2 can cleave the peptide between W and L residues with high catalytic efficiency.…”
Section: Biosensors Based On Targeting Peptides As Recognition Elementsmentioning
confidence: 99%