Quantum dots functionalized with 3-mercaptophenylboronic acids as novel nanoplatforms to evaluate sialic acid content on cell membranes

Monteiro, Camila A. P.; Silva, Ryan C.; Assis, Lara G.; Pereira, Goreti; Pereira, Giovannia A. L.; Santos, Beate S.; Filho, Paulo E. Cabral; Fontes, Adriana

doi:10.1016/j.colsurfb.2020.111142

Cited by 9 publications

(3 citation statements)

References 36 publications

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“…Compared with the reported fluorescence methods for sugar detection based on boric acid (Table S3), this fluorescence biosensor has a lower LOD and wider detection range. ,− In addition, this method is simple, effective, and time-saving than the existing methods, thus contributing to wider application in the clinics and laboratories.…”

Section: Principle Of the Fluorescent Biosensormentioning

confidence: 99%

Screening of a Sialyllactose-Specific Aptamer and Engineering a Pair of Recognition Elements with Unique Fluorescent Characteristics for Sensitive Detection of Sialyllactose

Chen

Zhang

et al. 2023

J. Agric. Food Chem.

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A single-stranded DNA (ssDNA) aptamer specific for 6′-sialyllactose (6′-SL) was screened through magnetic separation-based SELEX and post-SELEX truncation and used to construct unique aptamer bio-dots for sensitive detection of 6′-SL. Eighteen rounds of screening were conducted during the SELEX process. The ssDNA aptamer Apt9 (K d = 152.3 nM) with a length of 79 nucleotides (nt) was demonstrated as the optimal aptamer candidate after affinity and specificity evaluation. Then, Apt9 was truncated and optimized according to secondary structure and molecular docking. A 35 nt truncated aptamer Apt9-1 (K d = 91.75 nM) with higher affinity than Apt9 was finally obtained. Furthermore, Apt9-1 was used to synthesize bio-dots as a new recognition element of 6′-SL, and the aminobenzene boric acid functionalized carbon dots were employed as the other recognition element. With the respective fluorescent characteristics, the two quantum dots (QDs) were made a pair to construct a 6′-SL fluorescent biosensor. The linear detection range of the biosensor is 10 μM to 5 mM, and the detection limit is 0.9 μM. With the advantages of time-saving, high efficiency, and simplicity in the actual sample detection, the screened aptamer and dual-QD-based biosensor have broad application prospects in 6′-SL detection.

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Section: Principle Of the Fluorescent Biosensormentioning

confidence: 99%

Screening of a Sialyllactose-Specific Aptamer and Engineering a Pair of Recognition Elements with Unique Fluorescent Characteristics for Sensitive Detection of Sialyllactose

Chen

Zhang

et al. 2023

J. Agric. Food Chem.

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“…6.0. The sizes of the nanosystems were obtained by Fluorescence Correlation Spectroscopy (FCS), following the procedure reported by Monteiro et al [46].…”

Section: Evaluation Of the Conjugation Efficiencymentioning

confidence: 99%

Mannose-binding lectin conjugated to quantum dots as fluorescent nanotools for carbohydrate tracing

Lima

Oliveira

Silva

et al. 2022

Methods Appl. Fluoresc.

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Quantum dots (QDs) have stood out as nanotools for glycobiology due to their photostability and ability to be combined with lectins. Mannose-binding lectin (MBL) is involved in the innate immune system and plays important roles in the activation of the complement cascade, opsonization, and elimination of apoptotic and microbial cells. Herein, adsorption and covalent coupling strategies were evaluated to conjugate QDs to a recombinant human MBL (rhMBL). The most efficient nanoprobe was selected by evaluating the conjugate ability to label Candida albicans yeasts by flow cytometry. The QDs-rhMBL conjugate obtained by adsorption at pH 6.0 was the most efficient, labeling ca. 100% of cells with the highest median fluorescence intensity. The conjugation was also supported by Fourier transform infrared spectroscopy, zeta potential, and size analyses. C. albicans labeling was calcium-dependent; 12% and <1% of cells were labeled in buffers without calcium and containing EDTA, respectively. The conjugate promoted specific labeling (based on cluster effect) since, after inhibition with mannan, there was a reduction of 80% in cell labeling, which did not occur with methyl-α-D-mannopyranoside monosaccharide. Conjugates maintained colloidal stability, bright fluorescence, and biological activity for at least 8 months. Therefore, QDs-rhMBL conjugates are promising nanotools to elucidate the roles of MBL in biological processes.

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“…The content of SA on the membrane of cancer cells is significantly higher than that of normal cells, which makes it a tumor marker. The diagnosis and treatment of tumors based on SA has important clinical value ( Monteiro et al, 2020 ). Under weakly acidic conditions of tumor tissue, PBA and SA can form stable borates.…”

Section: Introductionmentioning

confidence: 99%

Targeted delivery of miR-34a-5p by phenylborate-coupled polyethylenimide nanocarriers for anti-KSHV treatment

Li,

Cao,

Yao

et al. 2024

Front. Bioeng. Biotechnol.

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Kaposi’s sarcoma-associated herpesvirus (KSHV) can infect a variety of cells and cause malignant tumors. At present, the use of microRNA (miRNA) for anti-KSHV is a promising treatment strategy, but the instability and non-specific uptake of miRNA still limit its use in the treatment of KSHV. In the present study, we constructed a nano-drug delivery system employing chemical grafting and electrostatic adsorption to solve the problems of easy degradation and low cell uptake of miRNA during direct administration. This nano-drug delivery system is to graft 4-carboxyphenylboric acid (PBA) and lauric acid (LA) onto polyethylenimine (PEI) through amidation reaction, and then prepare cationic copolymer nanocarriers (LA-PEI-PBA). The drug-carrying nanocomplex LA-PEI-PBA/miR-34a-5p was formed after further electrostatic adsorption of miR-34a-5p on the carrier and could protect miR-34a-5p from nuclease and serum degradation. Modification of the drug-carrying nanocomplex LA-PEI-PBA/miR-34a-5p by targeted molecule PBA showed effective uptake, increase in the level of miR-34a-5p, and inhibition of cell proliferation and migration in KSHV-infected cells. In addition, the drug-carrying nanocomplex could also significantly reduce the expression of KSHV lytic and latent genes, achieving the purpose of anti-KSHV treatment. In conclusion, these cationic copolymer nanocarriers with PBA targeting possess potential applications in nucleic acid delivery and anti-KSHV therapy.

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Quantum dots functionalized with 3-mercaptophenylboronic acids as novel nanoplatforms to evaluate sialic acid content on cell membranes

Cited by 9 publications

References 36 publications

Screening of a Sialyllactose-Specific Aptamer and Engineering a Pair of Recognition Elements with Unique Fluorescent Characteristics for Sensitive Detection of Sialyllactose

Screening of a Sialyllactose-Specific Aptamer and Engineering a Pair of Recognition Elements with Unique Fluorescent Characteristics for Sensitive Detection of Sialyllactose

Mannose-binding lectin conjugated to quantum dots as fluorescent nanotools for carbohydrate tracing

Targeted delivery of miR-34a-5p by phenylborate-coupled polyethylenimide nanocarriers for anti-KSHV treatment

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