Quartz crystal microbalance, a valuable tool for elucidation of interactions between apoB-100 peptides and extracellular matrix components

Abstract

Atherosclerosis has received wide attention as a primary cause of premature death in developed countries. The retention of low-density lipoprotein (LDL) particles in the intima, the inner layer of the capillaries, has been imputed as the main cause of the development of atherosclerotic plaques. The entrapment of LDL is mainly due to the specific interaction between the lysine-rich site on apolipoprotein B-100 (apoB-100), a major apolipoprotein of LDL, and extracellular matrix (ECM) components such as collagen, proteoglycans, and glycosaminoglycans (GAGs). Although valuable techniques already exist for studies on apoB-100 and ECM interactions, there is continued need for miniaturized tools that can complement the tools already available and even provide totally new data. This work explores the applicability of the quartz crystal microbalance (QCM) for interaction studies between apoB-100 peptide fragments and various components of the ECM. Two positive peptide fragments, PP and PP(2), and two components of the ECM, collagen I and a selected GAG, chondroitin 6-sulfate (C6S), were immobilized on polystyrene and carboxyl sensor chips. C6S was injected as analyte for PP- and PP(2)-coated surfaces, while PP was the analyte for collagen I and C(6)S surfaces. The estimated dissociation constant (K(D)) indicates that the interactions occur via the positive residues, lysine and arginine, of apoB-100. The continuous-flow QCM system employed in this study is shown to be an excellent tool for the elucidation of interactions between these types of biomolecules.

Preventing Obstructions of Nanosized Drug Delivery Systems by the Extracellular Matrix

“…QCM is characterized by high mass sensitivity; interactions between matrix components and particles may be measured with high accuracy. Interactions of biomolecules with collagen types have been measured by QCM . NMR analytics provide additional information about structure and dynamics within a short measurement time.…”

A Survey of the 2010 Quartz Crystal Microbalance Literature

“…Peptide‐protein interactions were studied by D'Ulivo et al . who looked at apolipoprotein B‐100, a low‐density lipoprotein implicated in atherosclerosis through its binding to extracellular matrix components such as collagen, proteoglycans and glycosaminoglycans (D'Ulivo et al ., ). Peptide fragments of the protein as well as matrix components were immobilised on the QCM and used to elucidate the binding affinities and interaction sites.…”

The evolution of selective analyses of HDL and LDL cholesterol in clinical and point of care testing

“…107,109,110 However, this was used to explore potential interactions between LDL and the vasculature, rather than to quantify LDL-C levels In addition, although QCM biosensors have been found to be very useful as laboratory-based investigative tools, their cost has Table 6 Percentage total error results of a single measurement based on the first result (no replication) for five homogeneous HDL-C and LDL-C assay methods 58 …”