Quartz crystal microbalance for immunosensing

Kößlinger, Conrad; Drost, S.; Aberl, Franz; Wolf, Hans

doi:10.1007/bf00326598

Cited by 36 publications

(21 citation statements)

References 21 publications

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“…The applicability of piezoimmunosensors in various fields is now well established A B [98]. The range extends from applications in clinical analysis [93,95,[99][100][101][102][103][104][105][106][107][108] through the determination of bacteria [94,[109][110][111][112][113][114] in the food industry to environmental analysis for the detection of organic compounds by using an antigen-antibody reaction [101,[115][116][117][118][119]. Since the invention of phage libraries, immunosensing systems based on the QCM have been successfully applied as devices for the screening of phage libraries and determination of antibody affinity [120][121][122].…”

Section: Piezoimmunosensorsmentioning

confidence: 99%

“…In order to minimize nonspecific adsorption, unoccupied active surface areas are successfully blocked by some nonactive proteins (bovine serumalbumin (BSA), gelatin or casein) before binding of the analyte. Additionally, nonspecific adsorption, which is predominantly caused by hydrophobic interactions, is reduced by the addition of detergents [103,105,107]. A comprehensive study of the impact of surface tension on the adsorption of proteins was given by Whitesides and co-workers [135].…”

Section: Piezoimmunosensorsmentioning

confidence: 99%

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Quartz Crystal Microbalance for Bioanalytical Applications

Janshoff

Steinem

2001

Sensors Update

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The quartz crystal microbalance (QCM) was first introduced as a mass sensor in gas phase and in vacuum. Since oscillator circuits capable of exciting shear vibrations of quartz resonators under liquid load have been developed, the QCM became accepted as a new powerful technique to monitor adsorption processes at solid/liquid interfaces in chemical and biological research rendering the method an attractive low‐cost alternative for bioanalytic applications. In the last decade, adsorption of biomolecules on functionalized surfaces turned out to be one of the paramount applications of piezoelectric transducers comprising the interaction of DNA and RNA with complementary strands, specific recognition of protein‐ligands by immobilized receptors, the detection of virus capsids, bacteria, mammalian cells and last but not least the development of complete immunosensors. Piezoelectric transducers allow a label‐free detection of molecules; they are more than mere mass sensors since the sensor response is also influenced by interfacial phenomena, viscoelastic properties of the adhered biomaterial, surface charges of adsorbed molecules and surface roughness. These new insights have recently been used to investigate the adhesion of cells, liposomes and proteins onto surfaces allowing to determine morphological changes of cells as a response to pharmacological substances and changes in the water content of biopolymers in situ. However, future will show whether the quartz crystal microbalance will assert itself against established label‐free sensor devices like surface plasmon resonance spectroscopy and interferometry.

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Section: Piezoimmunosensorsmentioning

confidence: 99%

Section: Piezoimmunosensorsmentioning

confidence: 99%

Quartz Crystal Microbalance for Bioanalytical Applications

Janshoff

Steinem

2001

Sensors Update

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“…Thus, anti human immunodeficiency virus antibodies were detected in serum with selectivity comparable to ELISA. 46 Similarly, respiratory syndrome virus infection 47 and African swine fever disease 48 were identified in porcine sera and Salmonella enteritidis in chicken meat and egg. 49 Detection of antibodies against the African swine fever virus was realized using the recombinant viral attachment protein p12; an enhanced response was achieved using peroxidase label and 4-chloro-1-naphthol as substrate leading to insoluble product precipitating at the sensing surface.…”

Section: Determination Of Proteinsmentioning

confidence: 98%

Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions

Skládal¹

2003

J. Braz. Chem. Soc.

109

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Esta revisão apresenta cristais piezoeletricos de quartzo como transductores aplicáveis no desenvolvimento de diferentes tipos de ensaios bioanaliticos. Os componentes do sistema de medidas para sensores tipo piezo são descritos juntamente com os sistemas de equipamentos comerciais existentes. Os biosensores piezo elétricos podem ser aplicados para determinação de vírus, bacteriais e outras células, proteínas, ácidos nucléicos e pequenas moléculas encontradas em remédios, hormônios e pesticidas. Além de mudança de massa, alguns ensaios de aglutinação empregando efeitos de viscosidade são discutidos. Finalmente, o monitoramento da afinidade das interações direto semmarcação e em tempo real usando sensores piezo é apresentado. Os pressupostos teóricos para a determinação apropriada das constantes de velocidade e de equilíbrio são apresentados e uma aproximação é demonstrada para a interação anticorpo com o correspondente antígeno (proteína secalin). Vários exemplos de estudo de afinidade são mostrados, incluindo a interação de proteínas (anticorpos e antígenos), de ácidos nucléicos (hibridização, intercalação de complexos metálicos), lipídeos e camadas com base de sacarídeos.This review presents piezoelectric quartz crystals as transducers suitable for development of different types of bioanalytical assays. The components of measuring systems for piezosensors are described together with providers of commercial equipment. The piezoelectric biosensors are summarized for determination of viruses, bacterial and other cells, proteins, nucleic acids and small molecules as drugs, hormones and pesticides. In addition to mass changes, some agglutination assays employing viscosity effects are addressed. Finally, the direct label-free and real-time monitoring of affinity interactions using piezosensors is presented. The theoretical background for determination of appropriate kinetic rate and equilibrium constants is shown and the approach is demonstrated on the interaction of antibody with the corresponding antigen (protein secalin). Several examples of affinity studies are provided, including interactions of proteins (antibody and antigens, receptors and ligands), nucleic acids (hybridization, intercalation of metal complexes), lipids and saccharide-based layers.

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“…To overcome this problem, synthetic peptides, which represent the epitope of an antibody, have been used instead of the whole biological macromolecule. These molecules were adsorbed on the gold surface of the sensor [85], or structured as (ordered) monolayers by thiol coupling [30]. These two approaches allow the detection of antibodies against the HIV virus [85] or against the foot-and-mouth disease virus [30].…”

Section: Affinity Sensors Based On Immunoreactionsmentioning

confidence: 99%

“…These molecules were adsorbed on the gold surface of the sensor [85], or structured as (ordered) monolayers by thiol coupling [30]. These two approaches allow the detection of antibodies against the HIV virus [85] or against the foot-and-mouth disease virus [30]. In both cases peptides represent a continuous epitope of a capsid protein of the corresponding virus.…”

Section: Affinity Sensors Based On Immunoreactionsmentioning

confidence: 99%