Quaternary ammonium chitosan derivative dynamic coating for the separation of veterinary sulfonamide residues by CE with field‐amplified sample injection

Abstract

A quaternary ammonium chitosan, 2-hydroxypropyltrimethylammonium chloride chitosan (HACC), has been developed for the dynamic coating material in CE for the first time. It presented many advantages such as favorable water solubility, satisfactory coating efficiency, and EOF toward the anode at pH >7.0. Using the modified fused-silica capillary, sulfonamides (SAs), an important group of veterinary drugs, were separated and detected by CE combined with field-amplified sample injection (FASI). The LODs of sulfonamides with UV detection were less than 0.5 ng/mL. The proposed method has been applied to the determination of veterinary sulfonamide residues in samples such as chicken, beef, and honey with fast separation (15 sulfonamides within 20 min), low LODs (0.1-0.5 ng/mL), and good reliability compared to the criteria of China (GB/T 18932.17-2003).

Analyses of sulfonamide antibiotics by a successive anion‐ and cation‐selective injection coupled to microemulsion electrokinetic chromatography

“…Other on‐line concentration CE‐UV studies of the sulfonamides reported the best LODs in the range of 0.1–0.5 μg/L, in which a fused‐silica capillary coated with a specific quaternary ammonium chitosan was needed 34. This study demonstrated that the SACSI step coupled to the MEEKC method provided the sulfonamides comparable detection sensitivities, where no complicated column pretreatment was needed.…”

“…After the n ‐hexane organic layer was discarded, the ACN‐aqueous liquid was collected and then was dried with oven at 120°C. This extraction procedure was followed as in the previous method 34, but some part was modified in the study.…”

“…Owing to its inadequate detection sensitivity, off‐line (SPE) 26, 27 and on‐line preconcentration 28–30 techniques were often coupled with the CE‐UV systems for trace‐level antibiotics determination. Presently, several highly sensitive CE methods, which used on‐line concentration strategy, have been reported for the analyses of sulfonamide at low microgram per liter levels 31–34. Among them, Ma et al .…”

“…Among them, Ma et al . used the field‐amplified sample injection strategy to analyze sulfonamide residues in food samples, and their proposed procedure provided the best detection ability for sulfonamide antibiotics thus far (LODs at 0.1–0.5 μg/L range) 34. In this case, a quaternary ammonium chitosan, 2‐hydroxypropyltrimethylammonium chloride chitosan, was used as the dynamic coating material of the capillary in order to provide stable reversed EOF and good separation efficiency.…”

Aplicações da eletroforese capilar na análise do biomarcadir alfa-1 glicoproteína ácida, no controle de qualidade do biofármaco interferon alfa 2a e na avaliação da estabilidade enantiosseletiva do fármaco isradipina

“…Comparada às demais técnicas de eletromigração, poucas aplicações foram encontradas utilizando MEKC para análise de proteínas. Segundo alguns autores, MEKC é adequada para a separação de proteínas, peptídeos e outros biopolímeros que apresentam estruturas ou pI semelhantes (LA et al, 2002;HOLLAND;LEIGH, 2003 SUN, 2005;MA et al, 2007;WU et al, 2009).…”