QuEChERS-Based Approach to the Extraction of Five Calcium Channel Blockers from Plasma Determined by UPLC-MS/MS

Zhao, Tingting; Jiang, Wen; Zhen, Xiaolan; Jin, Chengcheng; Zhang, Yifan; Li, Hui

doi:10.3390/molecules28020671

Cited by 5 publications

(3 citation statements)

References 23 publications

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“…felodipine, nicardipine, etc. ), which predominantly affect the vascular smooth muscle, and non-dihydropyridine CCBs (diltiazem, verapamil), which act mainly on myocardial L-type channels (32). There is evidence that different CCBs may have different effect on mood disorders.…”

Section: Discussionmentioning

confidence: 99%

Causal relationship between genetic proxies for calcium channel blockers and the risk of depression: a drug-target Mendelian randomization study

Ye,

Wang,

Wang

et al. 2024

Front. Psychiatry

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BackgroundCalcium channel blockers (CCBs) are widely used in the clinical management of hypertension. Depression, a common comorbidity of hypertension, is an important issue in the management of hypertension. However, the impact of CCBs on depression risk remains controversial. We aim to investigate the causal effect of CCBs on depression through drug-target Mendelian randomization (MR) analysis.MethodsTo proxy CCBs, we utilized the genetic variations located in or around drug target genes that were related to systolic blood pressure (SBP). Coronary artery disease (CAD) served as the positive control outcome. Genetic summary data of SBP, CAD, and depression were obtained from genome-wide association studies (GWAS) based on European population. Inverse variance weighted (IVW) method was applied as the main analysis to estimate the causal effect. Cochran’s Q test, MR-Egger intercept, MR pleiotropy residual sum and outlier (MR-PRESSO) and leave-one-out sensitivity analysis were used to test the robustness of the results. Meta-analysis was applied to further confirm whether causal relationships existed between CCBs and depression.ResultsThe IVW results failed to reveal any causal relationship between genetic proxies for CCBs and depression (P > 0.05). Cochran’s Q test showed no evidence of heterogeneity (P > 0.05). The MR-Egger intercept test suggested no evidence of directional pleiotropy, and the MR pleiotropy residual sum and outlier (MR-PRESSO) global test for horizontal pleiotropy was also not significant (P > 0.05). Leave-one-out analysis did not reveal any genetic variant that influenced the results. In addition, the meta-analysis further confirmed the absence of a causal relationship.ConclusionThe present study indicates no association of genetic proxies for CCBs with depression. Further studies are necessary to provide definitive evidence.

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Section: Discussionmentioning

confidence: 99%

Causal relationship between genetic proxies for calcium channel blockers and the risk of depression: a drug-target Mendelian randomization study

Ye,

Wang,

Wang

et al. 2024

Front. Psychiatry

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“…From the literature survey, various approaches have been reported for the estimation of NIF, CAP and BIS alone or in combination with other drugs. The reported methods include HPLC (Ahmed et al, 2018; Andal et al, 2022; Bahgat et al, 2022; Kondratova et al, 2017; Rashid et al, 2022; Rastkari et al, 2013; Sultan et al, 2013; Sultana et al, 2011; Tatar Ulu & Aydoğmuş, 2012), LC–MS/MS (Giebułtowicz et al, 2015; Grigoriev et al, 2016; Logoyda, 2020; Logoyda et al, 2020; Logoyda et al, 2021; Todevska et al, 2022; Tonooka‐Kubota et al, 2022; Tutunji et al, 2009; Zhao et al, 2023), HPTLC (Aayushi & Shah, 2022; Chitlange et al, 2009; Kakde et al, 2008; Shah et al, 2019), spectrofluorimetric (Abd Elhaleem et al, 2022; Abdelwahab et al, 2018; Attia et al, 2023; Derayea et al, 2022; Dewangan et al, 2022; Dong et al, 2022; Han et al, 2022; Khan et al, 2014; Roshdy et al, 2023; Sheng et al, 2022; Wang et al, 2022; Wu et al, 2022; Xi et al, 2022), UV spectrophotometric (Abed, 2020; Gouda & Amin, 2010; Gudruman et al, 2012; Hamad et al, 2023; Mishra, 2022; Tulasamma & Venkateswarlu, 2016; Ulu & Kel, 2012) and electrochemical methods (Al‐Farhan et al, 2023; Frag et al, 2019; Górska et al, 2022; Hassan et al, 2022; Karimi‐Maleh et al, 2017; Khairy et al, 2017; Koçak & İpek, 2022; Mokhtari et al, 2018; Mourad et al, 2020; Pourhakkak et al, 2022; Soomro et al, 2017; Zare et al, …”

Section: Introductionmentioning

confidence: 99%

LC–MS/MS bioanalytical method for the quantitative analysis of nifedipine, bisoprolol, and captopril in human plasma: Application to pharmacokinetic studies

Abdel‐Megied

Kovalenko

Elbarbry

et al. 2023

Biomedical Chromatography

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In this study, the development and validation of an accurate and highly sensitive LC–MS/MS method were performed for the estimation of nifedipine, bisoprolol and captopril in real human plasma. Liquid–liquid extraction using tert‐butyl methyl ether was efficiently applied for extraction of the analytes from plasma samples. The chromatographic separation was carried out using an isocratic elution mode on the X‐terra MS C18 column (4.6 × 50 mm, 3.5 μm). The mobile phase consisted of methanol–0.1% formic acid (95:5, v/v) for determination of nifedipine and bisoprolol and acetonitrile–0.1% formic acid (70:30, v/v) for determination of captopril with a flow rate of 0.5 ml/min. Acceptable results regarding the different validation characteristics of the analytes were obtained in accordance with US Food and Drug Administration recommendations for bioanalytical methods. The developed approach was linear over concentration ranges of 0.5–130.0, 50.0–4,500.0 and 0.3–30.0 ng/ml for nifedipine, captopril and bisoprolol, respectively. The method revealed a sufficient lower limit of quantification in the range of 0.3–50.0 ng/ml, as well as high recovery percentages, indicating high bioanalytical applicability. The proposed method was efficiently applied to a pharmacokinetic evaluation of a fixed‐dose combination of the analytes in healthy male volunteers.

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“…The methods for the selective detection of nifedipine in the biological fluids are high-performance liquid chromatography (HPLC) coupled with a UV detector, 7 gas chromatography (GC), 8,9 liquid chromatography-mass spectrometry (LC-MS), 9,10 spectrofluorimetry, 11,12 and electrochemical methods. 13,14 However, chromatographic methods have some disadvantages such as the length of time required for sample identification, the high cost, the need for sample pretreatment, the need for trained technicians, and in some cases, the low sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Sensing Platform Based on the Modification of Carbon-Ceramic Electrode with Bimetallic Fe/Mn-MOF Applied in the Determination of Nifedipine in Human Serum Samples

Majidi,

Maleki,

Fadakar Bajeh Baj

2023

ImmunoAnalysis

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Background: Nifedipine, known as a calcium channel blocker, is widely used for the treatment of cardiovascular diseases such as hypertension and angina pectoris. However, its high doses are toxic and may result in a heartbeat, nausea, vomiting, etc. Methods: In the present research, the bimetallic Fe/Mn-MOF modified carbon-ceramic electrode (CCE) was used for studying the electrochemical behavior and determination of nifedipine. The presence of Fe/Mn-MOF on the surface of the CCE as an efficient modifier caused an increase in the current density of nifedipine oxidation. In this way, optimization of some effective parameters consisting of buffer type, pH, modifier concentration, etc. was successfully performed. Moreover, other analytical parameters including reproducibility, repeatability, stability, and selectivity were investigated. Results: The relative standard deviation (RSD) of peak current was 3.2 % for 10 µmol.L-1 nifedipine. Meantime, the linear range of 1-200 µmol.L-1. Additionally, limit of detection (LOD) and limit of quantification were obtained 0.24 μmol.L-1 and 0.82 µmol.L-1 respectively. The results showed that the modified electrode has a good electro-oxidation capability for nifedipine. Finally, for investigating the accuracy of the fabricated sensing assay, the determination of nifedipine in drug and human serum samples was performed. The recoveries and the related RSDs% were obtained 96.4-102% and 2.2-3.6% respectively. Conclusion: This modified electrode exhibited appropriate stability, low cost, simple preparation, fast response time, high repeatability, and good sensitivity. The obtained results clearly demonstrate the applicability of the proposed sensing assay in various matrix effects which does not affect the electrochemical response.

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QuEChERS-Based Approach to the Extraction of Five Calcium Channel Blockers from Plasma Determined by UPLC-MS/MS

Cited by 5 publications

References 23 publications

Causal relationship between genetic proxies for calcium channel blockers and the risk of depression: a drug-target Mendelian randomization study

Causal relationship between genetic proxies for calcium channel blockers and the risk of depression: a drug-target Mendelian randomization study

LC–MS/MS bioanalytical method for the quantitative analysis of nifedipine, bisoprolol, and captopril in human plasma: Application to pharmacokinetic studies

Electrochemical Sensing Platform Based on the Modification of Carbon-Ceramic Electrode with Bimetallic Fe/Mn-MOF Applied in the Determination of Nifedipine in Human Serum Samples

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