QuEChERS-based study on residue determination and dissipation of three herbicides in corn fields using HPLC-MS/MS

Zhong, Mengmeng; Wang, Tielong; Dong, Bizhang; Hu, Jiye

doi:10.1080/02772248.2015.1118479

Cited by 7 publications

(1 citation statement)

References 28 publications

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“…Monitoring of HM and other sulfonylureas in food samples is typically accomplished using instrumental methods, such as liquid chromatography-tandem mass spectrometry (LC-MS/MS) [2,5], capillary electrophoresis (CE)-MS/MS [3], and ultra-pressure liquid chromatography (UPLC) [6]. While these methods are sensitive and accurate, they are time intensive and require operation by specialized personnel.…”

Section: Introductionmentioning

confidence: 99%

Development of Magnetic Lateral Flow and Direct Competitive Immunoassays for Sensitive and Specific Detection of Halosulfuron-Methyl Using a Novel Hapten and Monoclonal Antibody

Ying

Cui

et al. 2023

Foods

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Halosulfuron-methyl (HM) is widely used for the removal of noxious weeds in corn, sugarcane, wheat, rice, and tomato fields. Despite its high efficiency and low toxicity, drift to nontarget crops and leaching of its metabolites to groundwater pose potential risks. Considering the instability of HM, the pyrazole sulfonamide of HM was used to generate a hapten and antigen to raise a high-quality monoclonal antibody (Mab, designated 1A91H11) against HM. A direct competitive immunoassay (dcELISA) using Mab 1A91H11 achieved a half-maximal inhibitory concentration (IC50) of 1.5 × 10−3 mg/kg and a linear range of 0.7 × 10−3 mg/kg–10.7 × 10−3 mg/kg, which was 10 times more sensitive than a comparable indirect competitive ELISA (icELISA) and more simple to operate. A spiking recovery experiment performed in tomato and maize matrices with 0.01, 0.05, and 0.1 mg/kg HM had average recoveries within 78.9–87.9% and 103.0–107.4% and coefficients of variation from 1.1–6.8% and 2.7–6.4% in tomato and maize, respectively. In addition, a magnetic lateral flow immunoassay (MLFIA) was developed for quantitative detection of low concentrations of HM in paddy water. Compared with dcELISA, the MLFIA exhibited 3.3- to 50-fold higher sensitivity (IC50 0.21 × 10−3 mg/kg). The average recovery and RSD of the developed MLFIA ranged from 81.5 to 92.5% and 5.4 to 9.7%. The results of this study demonstrated that the developed dcELISA and MLFIA are suitable for rapid detection of HM residues in tomato and maize matrices and paddy water, respectively, with acceptable accuracy and precision.

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