2017
DOI: 10.1080/09540105.2017.1348491
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QuEChERS extraction followed by enzyme-linked immunosorbent assay for determination of deoxynivalenol and zearalenone in cereals

Abstract: A simple, rapid and cost-effective QuEChERS method for the determination of ZEN and DON by ELISA in cereals was developed. The single factor experiment was used to find the optimal QuEChERS parameters (extraction solvent, extraction method, extraction time, the QuEChERS composition, sorbent and clarification time) and extracts were analysed by ELISA. The optimised method was validated for selectivity, linearity, limits of detection, matrix effect and precision. The recovery values of ZEN and DON by ELISA were … Show more

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Cited by 17 publications
(11 citation statements)
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“…The ELISA method for determination of ZEN and DON was compared with a standard HPLC method. The values obtained from the two detection systems for ZEN and DON entirely fit an excellent linear relationship, with a regression equation of y = 1.0281x À 0.2897 (correlation coefficient is 0.9955) for ZEN and a regression equation of y = 0.9952x + 4.4193 (correlation coefficient is 0.9984) for DON, further confirming the reliability of ELISA [47].…”
Section: Application Of Quechers In Extraction Of Aflatoxinssupporting
confidence: 59%
“…The ELISA method for determination of ZEN and DON was compared with a standard HPLC method. The values obtained from the two detection systems for ZEN and DON entirely fit an excellent linear relationship, with a regression equation of y = 1.0281x À 0.2897 (correlation coefficient is 0.9955) for ZEN and a regression equation of y = 0.9952x + 4.4193 (correlation coefficient is 0.9984) for DON, further confirming the reliability of ELISA [47].…”
Section: Application Of Quechers In Extraction Of Aflatoxinssupporting
confidence: 59%
“…With the emergence of new technologies and novel materials, sample pretreatment methods are also developing. Table S2 [ 11 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , …”
Section: Sample Pretreatment Methodsmentioning
confidence: 99%
“…In addition, the system has the potential to be miniaturized and used for on-site, field application. There are several reports on the development of novel immunoassays for detection of ZEN in a static system [26][27][28][29] and in a flow system [30][31], however this work is the first report on ZEN detection using a fully automated immunoassay system.…”
Section: Introductionmentioning
confidence: 98%