Quercetin as a simple but versatile primer in dentin bonding

Abstract

Poor bonding stability remains the "Achilles' heel" of dentin bonding, a simple and effective approach to inhibit the activities of both exogenous collagenases and endogenous MMPs, and prevention of the growth of bacterial biofilm is therefore highly demanded. In the present study, a quercetin/ethanol solution was developed as a multifunctional primer to pretreat the dentin surface during adhesive restoration. The results showed that pretreatment with quercetin/ethanol solutions (0.5 and 1.0 wt%) on the dentin surface effectively preserved the bonding strength after one-month of collagenase aging (p < 0.05). Irrespective of aging, lower nanoleakage expression and less cohesive failure in dentin were observed in the quercetin-treated groups (p < 0.05). Compared with the control group, the in situ zymography test revealed that the 0.5 and 1.0 wt% quercetin-treated dentin effectively inhibited MMP activity, while their contact angles significantly increased. Live/dead bacterial staining and the MTT assay demonstrated that the 0.5 and 1.0 wt% quercetin groups exerted significant bactericidal effects while inhibiting biofilm growth of Streptococcus mutans (p < 0.05). We believe that a quercetin/ethanol solution might serve as a simple but versatile primer to provide dentists with a promising approach to obtain desirable bonding stability and to prevent secondary caries, thereby preventing the frequent replacement of resin-based restorations.

Polyphenols and Brazilian red propolis incorporated into a total-etching adhesive system help in maintaining bonding durability

“…Quercetin has a stronger collagen crosslinking effect and is 100 times less cytotoxic than glutaraldehyde. Quercetin also induces dentinogenic differentiation of dental pulp cells, it has an antibacterial effect by inhibiting the metabolic activity of S. mutans [ 29 ], and inhibits the action of dentin MMPs [ 41 ]. These are important properties because quercetin added to dental bonding agents can increase the resistance of collagen fibrils at the hybrid layer, prevent secondary caries, and induce dental hard tissue formation after placing composite restorations.…”

“…In addition to the cytoprotective effects of antioxidants, treatment of the dentin surface with antioxidants such as RES and quercetin has been shown to preserve adhesive microtensile strength [ 12 , 25 , 41 , 47 ]. However, this is the first study on adding RES and BRP to dental adhesives, and it was not possible to compare our results on bond strength with other studies.…”

The roughness of deciduous dentin surface and shear bond strength of glass ionomers in the treatment with four minimally invasive techniques

“…Four deciduous dentin samples (npg ¼ 1) were xed to aluminum stubs with double-sided adhesive carbon tape (SPI Supplies, United States). A scanning electron microscopy (JEOL, JSM-6610 LV, Japan) was used to obtain images of changes in roughness, 19,20 with the following settings: low vacuum mode at 10 Pa chamber pressure, an electron acceleration voltage of 20 kV detecting backscattered electrons at a magnication of 1000Â.…”

Influence of MMP inhibitors on bond strength of adhesive restorations: systematic review and meta-analysis

“…studies [8,[23][24][25][27][28][29]] from 2008 to 2018 were included in the meta-analysis ( Figure 1) and another 5 articles [32,[78][79][80][81] were included in the qualitative analysis.…”

“…The general analysis showed no significant difference between the groups studied, with p=0.063 (95% confidence interval: 0.049-1.906 and random effect model, I 2 = 82.25%). The qualitative analysis included 5 studies [32,[78][79][80][81] and the results showed that the use of MMP inhibitors does not affect initial bond strength values. However, the studies that performed a long-term evaluation [32,78,79,81] showed that the bond strength values were higher for the inhibitor-treated groups compared to untreated groups.…”