Quercetin conjugated superparamagnetic magnetite nanoparticles for in-vitro analysis of breast cancer cell lines for chemotherapy applications

Kumar, Selvaraj Rajesh; Priyatharshni, S.; Babu, Varukattu Nipun; Mangalaraj, D.; Viswanathan, C.; Kannan, Soundarapandian; Ponpandian, N.

doi:10.1016/j.jcis.2014.08.064

Cited by 113 publications

(31 citation statements)

References 44 publications

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“…Initially, the effect of AuNPs-Qu-5 and free Qu on cell viability was assessed by various in vitro assays, through which the IC 50 values of AuNPs-Qu-5 for MCF-7 and MDA-MB-231 were found to be 50 and 100 μM, respectively, and the free Qu IC 50 values for MCF-7 and MDA-MB-231 were 100 and 125 μM, respectively. In support to this evidence, Kumar et al demonstrated the significant induction of cytotoxicity of quercetin conjugated Fe 3 O 4 nanoparticles in MCF-7cells when compared with free quercetin 39. Free AuNPs did not show any significant cytotoxicity towards MCF-7 or MDA-MB-231 cell lines.…”

mentioning

confidence: 85%

Gold nanoparticles–conjugated quercetin induces apoptosis via inhibition of EGFR/PI3K/Akt–mediated pathway in breast cancer cell lines (MCF‐7 and MDA‐MB‐231)

Solaimuthu

Mukherjee

Das

et al. 2017

Cell Biochemistry & Function

154

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Epidermal growth factor plays a major role in breast cancer cell proliferation, survival, and metastasis. Quercetin, a bioactive flavonoid, is shown to exhibit anticarcinogenic effects against various cancers including breast cancer. Hence, the present study was designed to evaluate the effects of gold nanoparticles-conjugated quercetin (AuNPs-Qu-5) in MCF-7 and MDA-MB-231 breast cancer cell lines. Borohydride reduced AuNPs were synthesized and conjugated with quercetin to yield AuNPs-Qu-5. Both were thoroughly characterized by several physicochemical techniques, and their cytotoxic effects were assessed by MTT assay. Apoptotic studies such as DAPI, AO/EtBr dual staining, and annexin V-FITC staining were performed. AuNPs and AuNPs-Qu-5 were spherical with crystalline nature, and the size of particles range from 3.0 to 4.5 nm. AuNPs-Qu-5 exhibited lower IC value compared to free Qu. There was a considerable increase in apoptotic population with increased nuclear condensation seen upon treatment with AuNPs-Qu-5. To delineate the molecular mechanism behind its apoptotic role, we analysed the proteins involved in apoptosis and epidermal growth factor receptor (EGFR)-mediated PI3K/Akt/GSK-3β signalling by immunoblotting and immunocytochemistry. The pro-apoptotic proteins (Bax, Caspase-3) were found to be up regulated and anti-apoptotic protein (Bcl-2) was down regulated on treatment with AuNPs-Qu-5. Additionally, AuNPs-Qu-5 treatment inhibited the EGFR and its downstream signalling molecules PI3K/Akt/mTOR/GSK-3β. In conclusion, administration of AuNPs-Qu-5 in breast cancer cell lines curtails cell proliferation through induction of apoptosis and also suppresses EGFR signalling. AuNPs-Qu-5 is more potent than free quercetin in causing cancer cell death, and hence, this could be a potential drug delivery system in breast cancer therapy.

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confidence: 85%

Gold nanoparticles–conjugated quercetin induces apoptosis via inhibition of EGFR/PI3K/Akt–mediated pathway in breast cancer cell lines (MCF‐7 and MDA‐MB‐231)

Solaimuthu

Mukherjee

Das

et al. 2017

Cell Biochemistry & Function

154

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“…Accordingly, these limitations may overcome by means of the SPIONs [ 32 – 34 ]. Consequently, Kumar et al and Akal et al [ 35 , 36 ] conjugated quercetin with SPIONs. Both studies used an in vitro method to evaluate the effect of quercetin conjugated with SPIONs on cells.…”

Section: Introductionmentioning

confidence: 99%

Using superparamagnetic iron oxide nanoparticles to enhance bioavailability of quercetin in the intact rat brain

Najafabadi

Kazemipour

Esmaeili

et al. 2018

BMC Pharmacol Toxicol

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BackgroundQuercetin (QT) as a bioactive flavonoid has a potential therapeutic activity for numerous neuronal injuries and neurodegenerative diseases. However, the low absorption rate of QT, especially through the blood-brain barrier, restricts its bioactivity in the body. The current research took the advantage of superparamagnetic iron oxide nanoparticles (SPIONs) to enhance the bioavailability of quercetin.MethodsQuercetin conjugated with SPIONs was prepared by means of nanoprecipitation method and was characterized by X-ray diffractometer, scanning electron microscope, and Fourier transformed infrared spectrometer analyses. Wistar male rats were orally fed by gavage with QT and QT-SPION at 50 and 100 mg/kg daily doses for 7 days. Using high-performance liquid chromatography (HPLC) method, biodistribution of QT was evaluated in plasma and brain tissue.ResultsThe outcomes of this research revealed a higher concentration in the plasma and brain of the rats fed with QT-SPION in comparison to free QT.ConclusionThe results of this study confirm that SPION as a targeted drug delivery system enhances the bioavailability of quercetin in the brain about ten folds higher than free quercetin and could be used for the treatment of neurodegenerative disorders.

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“…GQAuNPs were more cytotoxic than free quercetin, suggesting that the degree of cytotoxicity for quercetin was increased by conjugation of quercetin to the GQAuNPs. Kumar et al [44] have previously showed the significant induction of cytotoxicity of quercetin conjugated Fe 3 O 4 nanoparticles in MCF-7 cells when compared with free quercetin. The results showed that the QTSK1 is less toxic compared to the other prepared nanoparticles (Q1-Q4).…”

Section: In Vitro Biological Activity Of Gqaunpsmentioning

confidence: 98%

“…This is the maximal permissible exposure of skin as per ANSI regulation for 808 nm and continuous mode irradiation. Finally, we need to achieve optimal efficiency with a single treatment to avoid inducing adaptation to heat stress (thermotolerance) due to the expression of heat shock proteins (HSP) [44]. In the case where tumor cells are thermoresistant, it is possible to combine the nanoparticles with HSP inhibitor or small interfering RNA for HSP silencing.…”

Section: In Vivo Evaluation Of Gqaunpsmentioning

confidence: 99%

Endemic Plants: From Design to a New Way of Smart Hybrid Nanomaterials for Green Nanomedicine Applications

Haddada¹,

Gerometta²,

Bialecki³

et al. 2018

J Nanomed Nanotechnol

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Quercetin conjugated superparamagnetic magnetite nanoparticles for in-vitro analysis of breast cancer cell lines for chemotherapy applications

Cited by 113 publications

References 44 publications

Gold nanoparticles–conjugated quercetin induces apoptosis via inhibition of EGFR/PI3K/Akt–mediated pathway in breast cancer cell lines (MCF‐7 and MDA‐MB‐231)

Gold nanoparticles–conjugated quercetin induces apoptosis via inhibition of EGFR/PI3K/Akt–mediated pathway in breast cancer cell lines (MCF‐7 and MDA‐MB‐231)

Using superparamagnetic iron oxide nanoparticles to enhance bioavailability of quercetin in the intact rat brain

Endemic Plants: From Design to a New Way of Smart Hybrid Nanomaterials for Green Nanomedicine Applications

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