Quercetin induced HepG2 cells apoptosis through ATM/JNK/STAT3 signaling pathways

Abstract

Liver cancer is the seventh most common malignant tumor in the world and is the second highest cause of death due to cancer. Quercetin, a flavonoid with low toxicity, widely exists in various fruits and vegetables. It has the potential to be a therapeutic agent against various cancers. This study aimed to demonstrate the anti-tumor effect of quercetin on HepG2 cells. Quercetin suppressed the HepG2 cell proliferation in a dose-dependent manner in cell viability assay. Induction of cell apoptosis was confirmed by apoptotic cells population (sub-G1 peak) detected by flow cytometer. A decrease in mitochondrial membrane potential and caspase-3 activation were also demonstrated in this study. Furthermore, quercetin induced HepG2 cell apoptosis through ROS-mediated phosphorylated ataxia-telangiectasia mutated, c-Jun Nterminal kinases, signal transducer, and activator of transcription 3 (STAT-3), and Bax signaling pathways. These resultssuggest that quercetin has the potential to become an effective drug against the tumor.

Potential Molecular Mechanism of Illicium simonsii Maxim Petroleum Ether Fraction in the Treatment of Hepatocellular Carcinoma

“…Flavonoids were the major class of the identified active constituents. Compounds with anti-HCC effects include glabridin [ 15 ], baohuoside I [ 16 ], kaempferol [ 17 ], isorhamnetin [ 18 ], morin hydrate [ 19 ], kaempferitrin [ 20 ], genistein [ 21 ], pectolinarigenin [ 22 ], quercetin [ 23 ], luteolin [ 24 ], tangeretin [ 25 ], calycosin [ 26 ], and hyperin [ 27 ]. The total ion mass spectrometry is shown in Figure 1 , and the structure of the most abundant flavonoid in the PEIM is shown in Figure 2 .…”

Research progress on natural products against hepatocellular carcinoma

“…Both chemical and surgical treatment of interventional therapy could not provide a good prognosis. Therefore, there is an urgency to find an effective way (natural products) to treat HCC [58].…”

Sciadopitysin exerts anticancer effects on HepG2 hepatocellular carcinoma cells by regulating reactive oxygen species-mediated signaling pathways

“…Cell apoptosis as a therapeutic approach for cancer has garnered considerable attention in recent years [9]. For example, Quercetin can induce apoptosis in HepG2 cells through ataxia telangiectasia mutated (ATM)/c-Jun Nterminal kinase (JNK)/signal transducer and activator of transcription 3 (STAT3) signaling pathway [10]. Mitochondria are essential components of eukaryotic cells.…”