Quercetin promotes the osteogenic differentiation of rat mesenchymal stem cells via mitogen-activated protein kinase signaling

Yang, Li; Wang, Jiefang; Chen, Guangming; Feng, Shuiwang; Wang, Panpan; Zhu, Xiaofeng; Zhang, Ronghua

doi:10.3892/etm.2015.2388

Cited by 50 publications

(35 citation statements)

References 20 publications

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“…Quercetin has been reported to enhance cell proliferation and osteogenic differentiation of mesenchymal stem cells in dosedependent manner by activation of ERK and MAPK pathway (Kim, Bae, Suh, & Jung, 2006;Li et al, 2015;Zhou & Lin, 2014;Zhou et al, 2015Zhou et al, , 2017. Notoya et al (2004) have also reported inhibitory effect of quercetin on the osteogenic differentiation.…”

Section: Discussionmentioning

confidence: 99%

Ethyl acetate and n‐butanol fraction of Cissus quadrangularis promotes the mineralization potential of murine pre‐osteoblast cell line MC3T3‐E1 (sub‐clone 4)

Toor

Tasadduq

Adhikari

et al. 2018

Journal Cellular Physiology

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In a sequel to investigate osteogenic potential of ethanolic extract of Cissus quadrangularis (CQ), the present study reports the osteoblast differentiation and mineralization potential of ethyl acetate (CQ‐EA) and butanol (CQ‐B) extracts of CQ on mouse pre‐osteoblast cell line MC3T3‐E1 (sub‐clone 4) with an objective to isolate an antiosteoporotic compound. Growth curve, proliferation, and viability assays showed that both the extracts were nontoxic to the cells even at high concentration (100 µg/ml). The cell proliferation was enhanced at low concentrations (0.1 µg/ml and 1 µg/ml) of both the extracts. They also upregulated the osteoblast differentiation and mineralization processes in MC3T3‐E1 cells as reflected by expression profile of osteoblast marker genes such as RUNX2, Osterix, Collagen (COL1A1), Alkaline Phosphatase (ALP), Integrin‐related Bone Sialoprotein (IBSP), Osteopontin (OPN), and Osteocalcin (OCN). CQ‐EA treatment resulted in early differentiation and mineralization as compared with the CQ‐B treatment. These findings suggest that low concentrations of CQ‐EA and CQ‐B have proliferative and osteogenic properties. CQ‐EA, however, is more potent osteogenic than CQ‐B.

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Section: Discussionmentioning

confidence: 99%

Ethyl acetate and n‐butanol fraction of Cissus quadrangularis promotes the mineralization potential of murine pre‐osteoblast cell line MC3T3‐E1 (sub‐clone 4)

Toor

Tasadduq

Adhikari

et al. 2018

Journal Cellular Physiology

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“…A large number of studies have been conducted on the effects of phytochemicals on the fate of stem cells. Currently, the phytochemicals studied mainly fall into the following categories: icariin [139], resveratrol [140], quercetin [141], and curcumin [142] (Table 2). Icariin is extracted from the plant herba epimedii and helps improve male fertility [143].…”

Section: Scaffold Chemical Cuesmentioning

confidence: 99%

“…The combination of resveratrol and BMMSCs could effectively suppress proinflammatory cytokines (IFNγ, TNF-α) and increase anti-inflammatory cytokines (IL-4, IL-10) [145]. Quercetin is one of the most ubiquitous bioflavonoids, widely found in many kinds of plants [141]. Quercetin has a positive pharmacological effect on bone metabolism, which could play a leading role in the quercetin-promoted osteogenic proliferation and differentiation of MSCs by activating the ERK1/2 and JNK signaling pathways [146].…”

Section: Scaffold Chemical Cuesmentioning

confidence: 99%

Regulation and Directing Stem Cell Fate by Tissue Engineering Functional Microenvironments: Scaffold Physical and Chemical Cues

Xing

Zhou

et al. 2019

Stem Cells International

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It is well known that stem cells reside within tissue engineering functional microenvironments that physically localize them and direct their stem cell fate. Recent efforts in the development of more complex and engineered scaffold technologies, together with new understanding of stem cell behavior in vitro, have provided a new impetus to study regulation and directing stem cell fate. A variety of tissue engineering technologies have been developed to regulate the fate of stem cells. Traditional methods to change the fate of stem cells are adding growth factors or some signaling pathways. In recent years, many studies have revealed that the geometrical microenvironment played an essential role in regulating the fate of stem cells, and the physical factors of scaffolds including mechanical properties, pore sizes, porosity, surface stiffness, three-dimensional structures, and mechanical stimulation may affect the fate of stem cells. Chemical factors such as cell-adhesive ligands and exogenous growth factors would also regulate the fate of stem cells. Understanding how these physical and chemical cues affect the fate of stem cells is essential for building more complex and controlled scaffolds for directing stem cell fate.

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“…Ghrelin-mediated activation of Erk1/2 pathway accelerated the growth of rabbit MSCs [35]. Quercetin was also demonstrated to promote the osteogenic differentiation of rat MSCs by activating MAPK cascade [36]. Thus, these findings implicate the Erk1/2-MAPK signaling to be an essential controller of bone formation.…”

Section: Cell Physiolmentioning

confidence: 66%

An Inhibitory Role of Osthole in Rat MSCs Osteogenic Differentiation and Proliferation via Wnt/β-Catenin and Erk1/2-MAPK Pathways

Chen

Dai

et al. 2016

Cell Physiol Biochem

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Background/Aims: Bone marrow-derived mesenchymal stem cells (MSCs) are responsible for new bone formation during adulthood. Accumulating evidences showed that Osthole promotes the osteogenic differentiation in primary osteoblasts. The aim of this study was to investigate whether Osthole exhibits a potential to stimulate the osteogenic differentiation of MSCs and the underlying mechanism. Methods: MSCs were treated with a gradient concentration of Osthole (6.25 µM, 12.5 µM, and 25 µM). Cell proliferation was assessed by western blotting with the proliferating cell nuclear antigen (PCNA) and Cyclin D1 antibodies, fluorescence activated cell sorting (FACS), and cell counting kit 8 (CCK8). MSCs were cultured in osteogenesis-induced medium for one or two weeks. The osteogenic differentiation of MSCs was estimated by Alkaline Phosphatase (ALP) staining, Alizarin red staining, Calcium influx, and quantitative PCR (qPCR). The underlying mechanism of Osthole-induced osteogenesis was further evaluated by western blotting with antibodies in Wnt/β-catenin, PI3K/Akt, BMPs/smad1/5/8, and MAPK signaling pathways. Results: Osthole inhibited proliferation of rat MSCs in a dose-dependent manner. Osthole suppressed osteogenic differentiation of rat MSCs by down-regulating the activities of Wnt/β-catenin and Erk1/2-MAPK signaling. Conclusions: Osthole inhibits the proliferation and osteogenic differentiation of rat MSCs, which might be mediated through blocking the Wnt/β-catenin and Erk1/2-MAPK signaling pathways.

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Quercetin promotes the osteogenic differentiation of rat mesenchymal stem cells via mitogen-activated protein kinase signaling

Cited by 50 publications

References 20 publications

Ethyl acetate and n‐butanol fraction of Cissus quadrangularis promotes the mineralization potential of murine pre‐osteoblast cell line MC3T3‐E1 (sub‐clone 4)

Ethyl acetate and n‐butanol fraction of Cissus quadrangularis promotes the mineralization potential of murine pre‐osteoblast cell line MC3T3‐E1 (sub‐clone 4)

Regulation and Directing Stem Cell Fate by Tissue Engineering Functional Microenvironments: Scaffold Physical and Chemical Cues

An Inhibitory Role of Osthole in Rat MSCs Osteogenic Differentiation and Proliferation via Wnt/β-Catenin and Erk1/2-MAPK Pathways

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