Quercetin with lycopene modulates enzymic antioxidant genes pathway in isoproterenol cardiotoxicity in rats

Abstract

Quercetin (QN) is a naturally occurring phenolic compound found largely in vegetables and fruits. Lycopene (LY) is yet another natural phytocompound, found abundantly in red-colored fruits and vegetables. Both have been reported to have beneficial activities in humans. In this study, we document in vivo experimental model for isoproterenol (ISO) cardiac injury toxicity in Sprague-Dawley (SD) rats and treatment with a combined optimized concentration of quercetin and lycopene (QL). Male SD rats of different groups were treated with QL (80 mg/kg QN and 3 mg/kg LY together p.o.) for 10 days with ISO administration (100 mg/kg i.p.) on days 7 and 8. After experimental period, CK-MB, TROP, AST, ALT, LDH, GST, GPx, CAT, SOD, Vit.E, Vit. C, GSH, GSSG and MDA were estimated. SD rats administered with ISO showed an obvious rise in the serum marker enzyme levels and tissue oxidative stress markers (MDA and GSSG). Furthermore, marked reductions in the body weight and increases enzymic and non-enzymic antioxidant levels were noticed. Histological features of the heart also indicated a disruption in the cardiac myofibrils structure of ISO-intoxicated rats. Also, quantitative PCR analysis revealed an involvement of antioxidant and related pathway genes such as Nrf2, HO-1, NQO1, GSTµ, SOD1, SOD2, CAT and BCl-2 genes. QL pretreatment prevented all these adverse effects of ISO cardiotoxicity and significantly reduced the myocardial damage. Decrease in oxidative stress was observed, possibly through alterations in the expression levels of enzymic antioxidant genes (GSTµ, SOD1, SOD2 and CAT). In general, QL exert a strong protective effect through the modulations in enzymic antioxidant activity and associated molecular pathways-regulating effect in cardiovascular disease.

Effect of Telmisartan and Quercetin in 5 Fluorouracil-Induced Renal Toxicity in Rats

“…All institutional and national guidelines for the care and use of laboratory animals were followed. The doses of quercetin, 27 telmisartan, 28 and 5-FU 29 were chosen depending on previous studies.…”

Targeting Cardiovascular Diseases by Flavonols: An Update

“…Flavonols have numerous cardioprotective properties, such as platelet stabilization, anti-inflammatory, antidiabetic, antihypertensive, and hypocholesterolemic activity [ 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 ]. To the best of our knowledge, quercetin is the most widely distributed and well-known flavonol obtained from natural sources, as well as the best-studied flavonol, in both in vitro and in vivo studies [ 51 ].…”

Effects of Lycopene Attenuating Injuries in Ischemia and Reperfusion

“…Besides, other studies have shown that quercetin can be used in combination with other antioxidants to protect against ischemic injury [100,101]. The combined application of lycopene and quercetin improved myocardial ischemic injuries in a rat model [102]. In contrast with quercetin treatment (100 mg/kg b.w.)…”

“…Reducing cardiotoxicity (↓CK-MB, LDH, MYO, and TROP) and oxidative stress (↓MDA) via ↑GSTμ, SOD1, SOD2, CAT, and Bcl-2[102] …”