Quercitrin alleviates cartilage extracellular matrix degradation and delays ACLT rat osteoarthritis development: An in vivo and in vitro study

Guo, Hanli; Wu, Yin; Zou, Ziling; Zhang, Chao; Sun, Ming‐Hui; Min, Lingtian; Yang, Lei; Kong, Ling–Yi

doi:10.1016/j.jare.2020.06.020

Cited by 47 publications

(25 citation statements)

References 61 publications

(43 reference statements)

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“…The unwanted cleavage due to the non-specificity of the sgRNA within the genome exerts significant limitations to the CRISPR/Cas9 system which can alter the function of a gene or induce genomic instability. Currently, several naturally occurring and genetically modified Cas9 enzymes and specific sgRNA designing tools have been developed to enhance site specific target cleavage 57 – 59 , however, the off-target effect is still considered as a major limiting factor 60 . In the current study, we demonstrate that genetic resistance to root parasitic weeds can be obtained using CRISPR/Cas9 mediated targeted mutagenesis of the MAX1 , a SL biosynthetic gene in tomato.…”

Section: Discussionmentioning

confidence: 99%

CRISPR/Cas9 mediated mutagenesis of MORE AXILLARY GROWTH 1 in tomato confers resistance to root parasitic weed Phelipanche aegyptiaca

Bari

Nassar

Aly

2021

Sci Rep

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Root parasitic weeds infect numerous economically important crops, affecting total yield quantity and quality. A lack of an efficient control method limits our ability to manage newly developing and more virulent races of root parasitic weeds. To control the parasite induced damage in most host crops, an innovative biotechnological approach is urgently required. Strigolactones (SLs) are plant hormones derived from carotenoids via a pathway involving the Carotenoid Cleavage Dioxygenase (CCD) 7, CCD8 and More Axillary Growth 1 (MAX1) genes. SLs act as branching inhibitory hormones and strictly required for the germination of root parasitic weeds. Here, we demonstrate that CRISPR/Cas9-mediated targted editing of SL biosynthetic gene MAX1, in tomato confers resistance against root parasitic weed Phelipanche aegyptiaca. We designed sgRNA to target the third exon of MAX1 in tomato plants using the CRISPR/Cas9 system. The T0 plants were edited very efficiently at the MAX1 target site without any non-specific off-target effects. Genotype analysis of T1 plants revealed that the introduced mutations were stably passed on to the next generation. Notably, MAX1-Cas9 heterozygous and homozygous T1 plants had similar morphological changes that include excessive growth of axillary bud, reduced plant height and adventitious root formation relative to wild type. Our results demonstrated that, MAX1-Cas9 mutant lines exhibit resistance against root parasitic weed P. aegyptiaca due to reduced SL (orobanchol) level. Moreover, the expression of carotenoid biosynthetic pathway gene PDS1 and total carotenoid level was altered, as compared to wild type plants. Taking into consideration, the impact of root parasitic weeds on the agricultural economy and the obstacle to prevent and eradicate them, the current study provides new aspects into the development of an efficient control method that could be used to avoid germination of root parasitic weeds.

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Section: Discussionmentioning

confidence: 99%

CRISPR/Cas9 mediated mutagenesis of MORE AXILLARY GROWTH 1 in tomato confers resistance to root parasitic weed Phelipanche aegyptiaca

Bari

Nassar

Aly

2021

Sci Rep

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“…However, the other cultivar of senna (ALFT cultivar) had the highest sennoside B content at full sunlight condition which suggests that different species or even cultivars have distinct responses to environmental changes in the metabolomics level. Besides, quercitrin had been uncovered to have bioactive such as antioxidant, anti-inflammation and enhance osteoblast [ 56 ]. It accumulated more in under canopy habitats, and had also been identified and quantified in seeds of Rh.…”

Section: Discussionmentioning

confidence: 99%

Non-target metabolomics revealed the differences between Rh. tanguticum plants growing under canopy and open habitats

et al. 2021

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Background Rheum tanguticum (Rh. tanguticum) is an important traditional Chinese medicine plant, “Dahuang”, which contains productive metabolites and occupies wide habitats on the Qinghai-Tibet plateau. Plants occupying wide habitats usually vary in phenotypes such as in morphology and metabolism, thereby developing into different ecotypes. Under canopy and open habitats are a pair of dissimilar habitats which possess Rh. tanguticum plants. However, few studies have focused on the effect of habitats on Rh. tanguticum growth, particularly combining morphological and metabolic changes. This study focused on Rh. tanguticum plants growing in under canopy and open habitats where morphology and metabolism changes were quantified using non-target metabolism methods. Results The obtained results indicated that the two dissimilar habitats led to Rh. tanguticum developing into two distinct ecotypes where the morphology and metabolism were simultaneously changed. Under canopy habitats bred morphologically smaller Rh. tanguticum plants which had a higher level of metabolites (22 out of 31) which included five flavonoids, four isoflavonoids, and three anthracenes. On the other hand, the open habitats produced morphologically larger Rh. tanguticum plants having a higher level of metabolites (9 out of 31) including four flavonoids. 6 of the 31 metabolites were predicted to have effect targets, include 4 represent for under canopy habitats and 2 for open habitats. Totally, 208 targets were connected, among which 42 were communal targets for both under canopy and open habitats represent compounds, and 100 and 66 were unique targets for under canopy superior compounds and open habitats superior compounds, respectively. In addition, aloe-emodin, emodin, chrysophanol, physcion, sennoside A and sennoside B were all more accumulated in under canopy habitats, and among which aloe-emodin, emodin, chrysophanol and physcion were significantly higher in under canopy habitats. Conclusions This study determined that Rh. tanguticum growing in under canopy and in open habitats developed into two distinct ecotypes with morphological and metabolic differences. Results of network pharmacology study has indicated that “Dahuang” coming from different habitats, such as under canopy and open habitats, are different in effect targets and thus may have different medicinal use. According to target metabolomics, under canopy habitats may grow better “Dahuang”.

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“…Most recently, in 2021, Guo et al [ 245 ] explored the effect of quercitrin in ostheoarthritis (OA). Molecular mechanisms of quercitrin on OA were studied in vitro in primary chondrocytes and SW1353 cells.…”

Section: Biological Activitymentioning

confidence: 99%

Stevia Genus: Phytochemistry and Biological Activities Update

et al. 2021

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The Stevia genus (Asteraceae) comprises around 230 species, distributed from the southern United States to the South American Andean region. Stevia rebaudiana, a Paraguayan herb that produces an intensely sweet diterpene glycoside called stevioside, is the most relevant member of this genus. Apart from S. rebaudiana, many other species belonging to the Stevia genus are considered medicinal and have been popularly used to treat different ailments. The members from this genus produce sesquiterpene lactones, diterpenes, longipinanes, and flavonoids as the main types of phytochemicals. Many pharmacological activities have been described for Stevia extracts and isolated compounds, antioxidant, antiparasitic, antiviral, anti-inflammatory, and antiproliferative activities being the most frequently mentioned. This review aims to present an update of the Stevia genus covering ethnobotanical aspects and traditional uses, phytochemistry, and biological activities of the extracts and isolated compounds.

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Quercitrin alleviates cartilage extracellular matrix degradation and delays ACLT rat osteoarthritis development: An in vivo and in vitro study

Abstract: Graphical abstract

Cited by 47 publications

References 61 publications

CRISPR/Cas9 mediated mutagenesis of MORE AXILLARY GROWTH 1 in tomato confers resistance to root parasitic weed Phelipanche aegyptiaca

CRISPR/Cas9 mediated mutagenesis of MORE AXILLARY GROWTH 1 in tomato confers resistance to root parasitic weed Phelipanche aegyptiaca

Non-target metabolomics revealed the differences between Rh. tanguticum plants growing under canopy and open habitats

Stevia Genus: Phytochemistry and Biological Activities Update

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