Quercus dilatata Lindl. ex Royle ameliorates BPA induced hepatotoxicity in Sprague Dawley rats

Kazmi, Syeda Tayyaba Batool; Majid, Muhammad; Maryam, Sonia; Rahat, Aymen; Ahmed, Madiha; Khan, Muhammad Rashid; Haq, İhsan Ul

doi:10.1016/j.biopha.2018.03.097

Cited by 33 publications

(19 citation statements)

References 46 publications

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“…On the other hand, polyphenol rich I. batatas extracts provided a fine line of defense to the rat liver against ROS. The current study is in agreement to the findings of Khan et al [ 67 ] and Kazmi et al [ 68 ]. Moreover, I. batatas extracts provided significant inhibition of free radicals in vitro , which fortifies their capabilities to provide a better line of defense in in vivo.…”

Section: Discussionsupporting

confidence: 94%

Ipomoea batatas L. Lam. ameliorates acute and chronic inflammations by suppressing inflammatory mediators, a comprehensive exploration using in vitro and in vivo models

Majid

Nasir

Zahra

et al. 2018

BMC Complement Altern Med

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BackgroundIpomoea batatas L. Lam. is a functional food and belongs to family Convolvulaceae. It is used as an antiinflammatory, aphrodisiac, antiasthmatic, anticonvalescent, antitumor, antanemic and antidiabetic agent by local communities. This study has been planned to evaluate its antiinflammatory and antiarthritic potentials.MethodsDry powder of I. batatas tuber and roots were extracted with ethyl acetate (IPT-EA, IPR-EA) and methanol (IPT-M, IPR-M), respectively. These extracts were tested for total phenolic and flavonoid contents (TPC and TFC), HPLC finger printing, multidimensional in vitro and in vivo antioxidant potential and albumin denaturation inhibition. Carrageenan-induced paw edema, croton oil-induced ear and anal edema inhibition and Complete Freund’s Adjuvant (CFA)-induced antiarthritic assays were executed at a dose of 300 mg/kg body weight on Sprague-Dawley rats. Serum levels of interleukins IL-1β and IL-6 and nitric oxide (NO) were assessed to measure the inhibition of inflammation.ResultsMaximal TPC (319.81 ± 14.20 μg GAE/mg dry extract) and TFC (208.77 ± 9.09 μg QE/mg DE) were estimated in IPR-EA extract. IPT-EA and IPR-EA yielded the maximum amounts of rutin (7.3 ± 1.12 and 4.5 ± 0.55), caffeic acid (1.60 ± 0.25 and 2.17 ± 0.26) and myricetin (2.7 ± 0.14 and 1.01 ± 0.08 μg/mg DE), respectively in HPLC-DAD analysis. All extracts showed dose dependent response in in vitro antioxidant assays. Best inhibition (76.92 ± 3.07%) of albumin denaturation was shown by IPT-EA in comparison to ibuprofen (79.48 ± 4.71%). IPR-EA exhibited highest edema inhibition in models of carrageenan-induced paw edema (79.11 ± 5.47%) and croton oil-induced ear and anal edema (72.01 ± 7.80% and 70.80 ± 4.94%, respectively). Significant inhibition of CFA-induced arthritic edema and arthritic score were observed by IPR-EA as compared to ibuprofen. Suppression of pro-inflammatory cytokines (IL-1β, IL-6) and NO levels was shown by IPR-EA and IPT-EA, respectively.ConclusionThese results depict that richness of polyphenols and phytoconstituents in I. batatas ameliorates oxidative stress and inflammation of acute and chronic nature. Dose dependent antioxidant potential and inhibition of inflammatory edema, pro-inflammatory cytokines and hematological, biochemical and histological changes prove I. batatas therapeutic potential as an antiinflammatory and antiarthritic agent.Graphical abstract Electronic supplementary materialThe online version of this article (10.1186/s12906-018-2279-5) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 94%

Ipomoea batatas L. Lam. ameliorates acute and chronic inflammations by suppressing inflammatory mediators, a comprehensive exploration using in vitro and in vivo models

Majid

Nasir

Zahra

et al. 2018

BMC Complement Altern Med

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“…The nontoxic nature of plant sanctioned its safety and suitability as alternative aphrodisiac source with satisfactory outcomes. Kazmi et al [63] have conducted similar type of genoprotective study on Q. dilatata.…”

Section: Discussionmentioning

confidence: 99%

Scientific Validation of Ethnomedicinal Use ofIpomoea batatasL. Lam. as Aphrodisiac and Gonadoprotective Agent against Bisphenol A Induced Testicular Toxicity in Male Sprague Dawley Rats

Majid

Ijaz

Baig

et al. 2019

BioMed Research International

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Sweet potato (Ipomoea batatas L. Lam.), known as “Shakarqandi” in Pakistan, is an imperative root vegetable with large size, traditionally used as aphrodisiac, antiprostatic, anti-inflammatory, antidiabetic, cardiotonic, and anticancer agent. Present study was conducted to gauge aphrodisiac potential of Ipomoea batatas ethyl acetate (IPT-EA, IPA-EA) and methanol (IPT-M, IPA-M) extracts from tuber and aerial part, respectively, via behavioral and biochemical tests and their possible protective role in BPA-induced gonadotoxicity at the dose 300 mg/kg in male Sprague Dawley rats. Phytochemical analysis was done qualitatively and quantitatively through total phenolic and flavonoid content (TPC and TFC) and high performance liquid chromatographic (HPLC-DAD) fingerprinting while antioxidant profiling used multimode in vitro assays. To calculate sexual excitement mount latency, intromission latency, mount frequency, intromission frequency, ejaculatory latency, and postejaculatory interval were examined while for biochemical ratification semen characteristics, levels of testosterone, follicle stimulating hormone (FSH), luteinizing hormone (LH), and estradiol were measured. Gonadoprotective ability was assessed through comet assay and histomorphological examination of testes. Qualitative analysis ensured the presence of phenols, flavonoids, tannins, anthocyanin, saponins, coumarins, terpenoids, and betacyanin. Quantitatively maximal TPC (304.32±7.20 μg GAE/mg dry extract) and TFC (214.77±4.09 μg QE/mg DE) were estimated in IPA-EA extract. IPT-EA yielded maximum rutin (7.3±0.12) and myricetin (2.7±0.14 μg/mg DE) while IPA-EA and IPA-M yielded maximum caffeic acid (4.05±0.22 and 1.92±0.17 μg/mg DE, respectively) in HPLC-DAD analysis. Extracts enhanced sexual excitement, improved semen quality, levels of testosterone, FSH, LH, and estradiol, and successfully attenuated toxic effects of BPA. Levels of endogenous antioxidant enzymes (CAT, SOD, POD, and GSH) were restored and NO abundance was minimized. Significant stimulation in sexual behavior, amelioration of toxicity symptoms, elevated spermatic production, raised viability, vitalized levels of gonadal hormones, maintained endogenous enzymes, genoprotection, and reformed testicular histology endorsed I. batatas as a better aphrodisiac alternative and gonadoprotective agent.

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“…The histological changes were quanti ed as normal, moderate and severe based on the hepatic cytoplasm in ammation, centrilobular necrosis, cellular hypertrophy, vacuolization and steatosis (Keppler et al 2007). The histopathological changes were quanti ed by two independent histopathologist and scored double blindly (Kazmi et al 2018). The Masson's trichrome staining was performed to assess the changes in the liver parenchyma following Paracetamol-induced liver injury ).…”

Section: Histopathological Study Of Liver Tissuesmentioning

confidence: 99%

Comprehensive in vivo and in silico approaches to explore the hepatoprotective activity of poncirin against paracetamol toxicity

Ullah

Khan

Bibi

et al. 2022

Naunyn-Schmiedeberg's Arch Pharmacol

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Purpose: In the present study, the Poncirin was evaluated against the Paracetamol-induced liver injury using in vivo and computational approaches. Methods: The Paracetamol was administered intraperitoneally to establish the liver injury in mice and subsequently, to investigate the hepatoprotective effect of the Poncirin on the liver injury. The effect of the Poncirin was evaluated against the liver injury markers and in ammatory cytokines. Similarly, in the present study the antioxidants and oxidative stress parameters were also assessed following paracetamol-induced liver injury. The histological studies following liver injury was also assessed using H and E staining, Masson's trichrome staining, and periodic acid Schiff staining.Results: The Poncirin markedly improved the antioxidant enzymes, while attenuated the oxidative stress markers and in ammatory cytokines. The Poncirin also markedly improved hematological parameters and electrolytes pro le. Furthermore, the Poncirin treatment signi cantly improved the histological parameters using H and E staining, Masson's trichrome and PAS staining compared to the control. The Poncirin treatment also improved the liver function tests and liver synthetic activity compared to Paracetamol treated group. The immunohistochemistry analysis revealed signi cant decrease in the in ammatory signaling protein such as NF-κB (nuclear factor kappa light chain enhancer of activated B cells), JNK (Jun N-terminal Kinase) and COX-2 (Cyclooxygenase-2) expression level compared to the Paracetamol treated group. The in silico approach was also used to analyze the potential activity and explore the underlying molecular mechanism of Poncirin. Conclusion:The Poncirin improved the sign and symptoms associated with the liver injury using both in vivo and computational approaches.

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Quercus dilatata Lindl. ex Royle ameliorates BPA induced hepatotoxicity in Sprague Dawley rats

Cited by 33 publications

References 46 publications

Ipomoea batatas L. Lam. ameliorates acute and chronic inflammations by suppressing inflammatory mediators, a comprehensive exploration using in vitro and in vivo models

Ipomoea batatas L. Lam. ameliorates acute and chronic inflammations by suppressing inflammatory mediators, a comprehensive exploration using in vitro and in vivo models

Scientific Validation of Ethnomedicinal Use ofIpomoea batatasL. Lam. as Aphrodisiac and Gonadoprotective Agent against Bisphenol A Induced Testicular Toxicity in Male Sprague Dawley Rats

Comprehensive in vivo and in silico approaches to explore the hepatoprotective activity of poncirin against paracetamol toxicity

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