Quick and efficient measurement of transient GUS expression using image analysis

Anderson, William F.; Arthur, L. Beaudet; Ozias‐Akins, Peggy

doi:10.1007/bf02669276

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…A number of techniques, either at the mRNA level or at the pro tein level, are used for the assay of foreign gene expression in transgenic plants. These techniques include Northern blotting, RT-PCR Western blotting, ELISA (Rocke and Jones 1997;Crowuther 2001), computer image analysis (Anderson et al 1994;De Novoa and Coles 1994), histochemical stain ), visualizing fluorescent protein (Koncz et al 1987;Harper et al 1999) or anthocyanin (Ludwing et al 1990) and herbicide application (Casas et al 1993).…”

Section: Foreign Gene Expression In To Plantsmentioning

confidence: 99%

Genetic Transformation of Plants

Jackson¹,

Linskens²

2003

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Section: Foreign Gene Expression In To Plantsmentioning

confidence: 99%

Genetic Transformation of Plants

Jackson¹,

Linskens²

2003

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“…Transient expression in peanut has been monitored most frequently with the~-glucuronidase (GUS or uidA) reporter gene. Quantitation of transient expression usually is achieved by counting the number of GUS-positive foci per unit area, either manually or using computer-based image analysis procedures (4,45,92). Transient expression can be detected within a few hours of free-DNA uptake when the gene is under the control of an appropriate promoter.…”

Section: Regeneration-and Transformationcompetent Tissuesmentioning

confidence: 99%

Progress in the Development of Tissue Culture and Transformation Methods Applicable to the Production of Transgenic Peanut

Ozias‐Akins¹,

Gill

2001

Peanut Science

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Genetic engineering is a tool for crop improvement that extends our access to beneficial traits beyond sexuallycompatible crosses. Genes from virtually any organism can be cloned and introduced into peanut. Gene function can be influenced by the regulatory elements used to control expression as well as the genome context of the integration site(s) where one or multiple copies of the trans genes are inserted. Methods for the production of transgenic peanut (Arachishypogaea L.) that are based on biological or direct DNA transfer have been developed over the last decade. The most reliable method for the introduction of foreign DNA is microprojectile bombardment of embryogenic tissue cultures. With the use of a selectable marker gene for hygromycin resistance, transgenic plants can be recovered in 12-14 mo. Transgenic peanuts resistant to the lesser cornstalk borer have been produced with the 'Dept. of Horticulture, Univ. of Georgia, Tifton Campus, Tifton, GA 31793-0748.*Corresponding author (ozias@tifton.cpes.peachnet.edu).Peanut Science (2001) 28:123-131 objective of reducing aflatoxin contamination by decreasing insect damage to developing pods. The future application of this tool to increase pest resistance and enhance quality traits in peanut has enormous potential.

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Sorghum Transformation

Zhao¹,

Tomes²

2003

Genetic Transformation of Plants

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Quick and efficient measurement of transient GUS expression using image analysis

Cited by 3 publications

References 10 publications

Genetic Transformation of Plants

Genetic Transformation of Plants

Progress in the Development of Tissue Culture and Transformation Methods Applicable to the Production of Transgenic Peanut

Sorghum Transformation

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