Quorum-quenching limits quorum-sensing exploitation by signal-negative invaders

Tannières, Mélanie; Lang, Julien; Barnier, Claudie; Shykoff, Jacqui A.; Faure, Denis

doi:10.1038/srep40126

Cited by 19 publications

(13 citation statements)

References 49 publications

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“…When expressed into the plant cell nucleus, the T‐DNA genes divert the host hormonal and metabolic pathways to provoke the development of galls or plant tumors (Deeken et al ., ). In previous work, we paid attention to specific metabolites, the opines, that accumulate in the A. tumefaciens ‐infected plant tumors (Lang et al ., ; El Sahili et al ., ; Marty et al ., ; Tannières et al ., ; Lang et al ., ; Vigouroux et al ., ). Opines, such as agrocinopines, mannopine, nopaline, and octopine, result from the condensation of sugars and amino and organic acids (Dessaux et al ., ).…”

Section: Introductionmentioning

confidence: 98%

The biotroph Agrobacterium tumefaciens thrives in tumors by exploiting a wide spectrum of plant host metabolites

et al. 2019

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Agrobacterium tumefaciens is a niche-constructing biotroph that exploits host plant metabolites.We combined metabolomics, transposon-sequencing (Tn-seq), transcriptomics, and reverse genetics to characterize A. tumefaciens pathways involved in the exploitation of resources from the Solanum lycopersicum host plant.Metabolomics of healthy stems and plant tumors revealed the common (e.g. sucrose, glutamate) and enriched (e.g. opines, c-aminobutyric acid (GABA), c-hydroxybutyric acid (GHB), pyruvate) metabolites that A. tumefaciens could use as nutrients. Tn-seq and transcriptomics pinpointed the genes that are crucial and/or upregulated when the pathogen grew on either sucrose (pgi, kdgA, pycA, cisY) or GHB (blcAB, pckA, eno, gpsA) as a carbon source. While sucrose assimilation involved the Entner-Doudoroff and tricarboxylic acid (TCA) pathways, GHB degradation required the blc genes, TCA cycle, and gluconeogenesis. The tumorenriched metabolite pyruvate is at the node connecting these pathways. Using reverse genetics, we showed that the blc, pckA, and pycA loci were important for aggressiveness (tumor weight), proliferation (bacterial charge), and/or fitness (competition between the constructed mutants and wild-type) of A. tumefaciens in plant tumors.This work highlighted how a biotroph mobilizes its central metabolism for exploiting a wide diversity of resources in a plant host. It further shows the complementarity of functional genome-wide scans by transcriptomics and Tn-seq to decipher the lifestyle of a plant pathogen.

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Section: Introductionmentioning

confidence: 98%

The biotroph Agrobacterium tumefaciens thrives in tumors by exploiting a wide spectrum of plant host metabolites

et al. 2019

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“…Aside from the cost of DNA replication, the transfer involves the establishment of a T4SS that consists of a large number of proteins and requires ATP to export the DNA/protein complex [ 6 , 66 ]. In agreement, an accR mutant, which constitutively expresses the T4SS for Ti plasmid conjugation, is impaired in growth yield as compared to its wild-type parent [ 67 ]. Clearly, conjugation has to be regulated, and it is tightly regulated in Agrobacterium .…”

Section: Quorum Sensing and Quorum Quenching As Two Parts Of An Inmentioning

confidence: 99%

“…By controlling QS-signal level, QQ could also contribute to reduce QS-signal hijacking by agrobacteria (called QS-hijackers) which do not produce any QS-signal but are able to use that produced by others for activating the transfer of their own Ti plasmid. This hypothesis was recently investigated by authors [ 67 ] who reported, for the first time, the role of QQ lactonases in policing QS-signal hijacking. They showed that the QQ-lactonase may attenuate dissemination of QS-signal hijacking Ti-plasmid in co-culture assays, in which QS-signal emitting strain and QS-signal negative strain, each carrying a Ti plasmid, and a recipient strain, were mixed.…”

Section: Quorum Sensing and Quorum Quenching As Two Parts Of An Inmentioning

confidence: 99%

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Quorum Sensing and Quorum Quenching in Agrobacterium: A “Go/No Go System”?

Dessaux

Faure

2018

Genes

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The pathogen Agrobacterium induces gall formation on a wide range of dicotyledonous plants. In this bacteria, most pathogenicity determinants are borne on the tumour inducing (Ti) plasmid. The conjugative transfer of this plasmid between agrobacteria is regulated by quorum sensing (QS). However, processes involved in the disturbance of QS also occur in this bacteria under the molecular form of a protein, TraM, inhibiting the sensing of the QS signals, and two lactonases BlcC (AttM) and AiiB that degrade the acylhomoserine lactone (AHL) QS signal. In the model Agrobacterium fabrum strain C58, several data, once integrated, strongly suggest that the QS regulation may not be reacting only to cell concentration. Rather, these QS elements in association with the quorum quenching (QQ) activities may constitute an integrated and complex “go/no go system” that finely controls the biologically costly transfer of the Ti plasmid in response to multiple environmental cues. This decision mechanism permits the bacteria to sense whether it is in a gall or not, in a living or decaying tumor, in stressed plant tissues, etc. In this scheme, the role of the lactonases selected and maintained in the course of Ti plasmid and agrobacterial evolution appears to be pivotal.

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“…How does a population of cells in a multicellular organism maintain its cell density? Basic understanding is coming from studies with unicellular organisms where cells release chemical signals in dependence of cell density leading to a corresponding regulation of the cell cycle 1 – 3 . Beside this molecule-based, i.e.…”

Section: Introductionmentioning

confidence: 99%

Endogenous physical regulation of population density in the freshwater protozoan Paramecium caudatum

Fels

2017

Sci Rep

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Studies confirm physical long-range cell-cell communication, most evidently based on electromagnetic fields. Effects concern induction or inhibition of cell growth. Their natural function is unclear. With the protozoan Paramecium caudatum I tested whether the signals regulate cell density and are electromagnetic. Up to 300 cells/mL, cell growth in clones of this study is decreasingly pronounced. Using cuvettes as chemical barriers enabling physical communication I placed 5 indicator cells/mL, the inducer populations, into smaller cuvettes that stand in bigger and contained 50, 100, 200 or 300 cells/mL. Under conditions of total darkness such pairs were mutually exposed for 48 hours. The hypothesis was that indicator cells, too, grow less the more neighbor cells there are. The bigger inducer populations were in the beginning the less they grew. The indicator populations grew accordingly; the more cells they were surrounded by the less they grew. The suppressing neighbors-effect disappeared when inner cuvettes were shielded by graphite known to shield electromagnetic radiation from GHz to PHz, i.e. to absorb energy from microwaves to light. These are the first results demonstrating non-contact physical quorum sensing for cell population density regulation. I assume rules intrinsic to electromagnetic fields interacting with matter and life.

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Quorum-quenching limits quorum-sensing exploitation by signal-negative invaders

Cited by 19 publications

References 49 publications

The biotroph Agrobacterium tumefaciens thrives in tumors by exploiting a wide spectrum of plant host metabolites

The biotroph Agrobacterium tumefaciens thrives in tumors by exploiting a wide spectrum of plant host metabolites

Quorum Sensing and Quorum Quenching in Agrobacterium: A “Go/No Go System”?

Endogenous physical regulation of population density in the freshwater protozoan Paramecium caudatum

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