Quorum Sensing Biosensors

Rai, Navneet; Rai, Ruchi; Venkatesh, K. V.

doi:10.1007/978-81-322-1982-8_16

Cited by 15 publications

(11 citation statements)

References 25 publications

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“…The reporter protein may be detected optically or electrochemically; for example, the measurement of an amount of the protein luciferase encoded by lux gene can be quantified via bioluminescence. QS biosensors serve as fast and reliable tools for detecting specific or various types of autoinductors (Wynendaele et al 2013;O'Connor et al 2015;Rai et al 2015). Examples of methods based on biosensors are listed below (Table 1).To detect AHLs, mutants of bacteria strains that naturally produce bioluminescence in response to AHL molecules may also be used.…”

Section: Measurement Of Ai Productionmentioning

confidence: 99%

Prevention of biofilm formation by quorum quenching

Paluch

Rewak‐Soroczyńska

Jędrusik

et al. 2020

Appl Microbiol Biotechnol

304

151

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Quorum sensing (QS) is a mechanism that enables microbial communication. It is based on the constant secretion of signaling molecules to the environment. The main role of QS is the regulation of vital processes in the cell such as virulence factor production or biofilm formation. Due to still growing bacterial resistance to antibiotics that have been overused, it is necessary to search for alternative antimicrobial therapies. One of them is quorum quenching (QQ) that disrupts microbial communication. QQ-driving molecules can decrease or even completely inhibit the production of virulence factors (including biofilm formation). There are few QQ strategies that comprise the use of the structural analogues of QS receptor autoinductors (AI). They may be found in nature or be designed and synthesized via chemical engineering. Many of the characterized QQ molecules are enzymes with the ability to degrade signaling molecules. They can also impede cellular signaling cascades. There are different techniques used for testing QS/QQ, including chromatography-mass spectroscopy, bioluminescence, chemiluminescence, fluorescence, electrochemistry, and colorimetry. They all enable qualitative and quantitative measurements of QS/QQ molecules. This article gathers the information about the mechanisms of QS and QQ, and their effect on microbial biofilm formation. Basic methods used to study QS/QQ, as well as the medical and biotechnological applications of QQ, are also described. Basis research methods are also described as well as medical and biotechnological application.

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Section: Measurement Of Ai Productionmentioning

confidence: 99%

Prevention of biofilm formation by quorum quenching

Paluch

Rewak‐Soroczyńska

Jędrusik

et al. 2020

Appl Microbiol Biotechnol

304

151

View full text Add to dashboard Cite

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“…Detection of quorum sensing molecules is often performed by using quorum sensing reporter bacteria strains, of which most are sensitive to either autoinducer-1 (AHL's) or autoinducer-2 (Steindler and Venturi, 2007 ; Rai et al, 2015 ). As mentioned previously, these bacterial reporters can be based on non-genetically modified or genetically-modified bacteria.…”

Section: Analytical Techniquesmentioning

confidence: 99%

Peptides as Quorum Sensing Molecules: Measurement Techniques and Obtained Levels In vitro and In vivo

et al. 2017

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The expression of certain bacterial genes is regulated in a cell-density dependent way, a phenomenon called quorum sensing. Both Gram-negative and Gram-positive bacteria use this type of communication, though the signal molecules (auto-inducers) used by them differ between both groups: Gram-negative bacteria use predominantly N-acyl homoserine lacton (AHL) molecules (autoinducer-1, AI-1) while Gram-positive bacteria use mainly peptides (autoinducer peptides, AIP or quorum sensing peptides). These quorum sensing molecules are not only involved in the inter-microbial communication, but can also possibly cross-talk directly or indirectly with their host. This review summarizes the currently applied analytical approaches for quorum sensing identification and quantification with additionally summarizing the experimentally found in vivo concentrations of these molecules in humans.

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“…Since the C. violaceum -based biosensor plate assay presented some limitations, such as a high detection threshold and an inability to detect any 3-hydroxy-substituted AHLs [ 52 ], HPLC-MS analysis of the supernatants of the four strains cultured in MB was performed. The production of AHL was confirmed in all bacterial strains ( Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

Use of Quorum Sensing Inhibition Strategies to Control Microfouling

et al. 2021

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Interfering with the quorum sensing bacterial communication systems has been proposed as a promising strategy to control bacterial biofilm formation, a key process in biofouling development. Appropriate in vitro biofilm-forming bacteria models are needed to establish screening methods for innovative anti-biofilm and anti-microfouling compounds. Four marine strains, two Pseudoalteromonas spp. and two Vibrio spp., were selected and studied with regard to their biofilm-forming capacity and sensitivity to quorum sensing (QS) inhibitors. Biofilm experiments were performed using two biofilm cultivation and quantification methods: the xCELLigence® system, which allows online monitoring of biofilm formation, and the active attachment model, which allows refreshment of the culture medium to obtain a strong biofilm that can be quantified with standard staining methods. Although all selected strains produced acyl-homoserine-lactone (AHL) QS signals, only the P. flavipulchra biofilm, measured with both quantification systems, was significantly reduced with the addition of the AHL-lactonase Aii20J without a significant effect on planktonic growth. Two-species biofilms containing P. flavipulchra were also affected by the addition of Aii20J, indicating an influence on the target bacterial strain as well as an indirect effect on the co-cultured bacterium. The use of xCELLigence® is proposed as a time-saving method to quantify biofilm formation and search for eco-friendly anti-microfouling compounds based on quorum sensing inhibition (QSI) strategies. The results obtained from these two in vitro biofilm formation methods revealed important differences in the response of biosensor bacteria to culture medium and conditions, indicating that several strains should be used simultaneously for screening purposes and the cultivation conditions should be carefully optimized for each specific purpose.

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Quorum Sensing Biosensors

Cited by 15 publications

References 25 publications

Prevention of biofilm formation by quorum quenching

Prevention of biofilm formation by quorum quenching

Peptides as Quorum Sensing Molecules: Measurement Techniques and Obtained Levels In vitro and In vivo

Use of Quorum Sensing Inhibition Strategies to Control Microfouling

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