Quorum sensing LuxS/autoinducer-2 inhibits Enterococcus faecalis biofilm formation ability

Yang, Yongmei; Li, Wenzhi; Hou, Benxiang; Zhang, Chen

doi:10.1590/1678-7757-2017-0566

Cited by 17 publications

(12 citation statements)

References 25 publications

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“…Also, DNA bands were affirmed and examined by agarose gel electrophoresis. The results revealed that all DNA successfully extracted and all isolates gave bands with molecular weight 1500bp (Yang et al, 2018) as shown in figure ( 4).…”

Section: Molecular Study 341 Dna Extractionmentioning

confidence: 94%

Molecular and Bacteriological Study of Enterococcus faecalis Isolated from Different Clinical Sources

Khdir

Mustafa

2020

ZJPAS

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Enterococci are Gram-positive bacteria that cause serious nosocomial infections, including urinary tract, bloodstream infections and endocarditis. During the period of September 2018 to February 2019, forty four isolates of E. faecalis were isolated from 826 clinical specimens including; 35(5.07%) isolates of E. faecalis from urine, 7(7.60%) isolates from high vaginal swab and 2(8.69%) isolates from blood patients in different hospitals. All isolates that described above were identified depending on cultural criteria, morphological criteria, biochemical tests and further confirmed by Vitek 2 compact systems. The results of eleven antimicrobial against obtained isolates revealed that 100% of E. faecalis were resistant to cefotaxime, vancomycin, amoxicillin and erythromycin. While, it were 100% sensitive to doxycycline, imipenem, and nitrofurantion. Whereas, most isolates were differ in their susceptibility to amikacin, gentamycin, tetracycline and azithromycin. On the other hand, the results of biofilm found that 13.63% of isolates were produce strong biofilm, 54.54% were produce moderate biofilm and 31.81% were produce weak biofilm. The results of molecular analysis by using PCR showed that isolated E. faecalis were carried 97.72%, 90.90%, 63.63% of ebpR, asa1, esp genes respectively.

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Section: Molecular Study 341 Dna Extractionmentioning

confidence: 94%

Molecular and Bacteriological Study of Enterococcus faecalis Isolated from Different Clinical Sources

Khdir

Mustafa

2020

ZJPAS

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“…Such strain-related variations have been reported regarding the role of luxS in E. faecalis . While the deletion of luxS in the strain ATCC 33186 resulted in increased biofilm formation [75], it resulted in opposite effects in the strain ATCC 29212 [76], and this reduction was only significant in 48h-old biofilms.…”

Section: Resultsmentioning

confidence: 99%

Trans-cinnamaldehyde attenuatesEnterococcus faecalisvirulence and inhibits biofilm formation

Ali

Matinlinna

Lévesque

et al. 2021

Preprint

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Enterococcus faecalis as an important nosocomial pathogen is critically implicated in the pathogenesis of endocarditis, urinary tract and surgical wound infections. Its major virulence attributes (biofilm formation, production of proteases and hemolytic toxins) enable it to cause extensive host tissue damage. With the alarming increase in enterococcal resistance to antibiotics, novel therapeutics are required to inhibit E. faecalis biofilm formation and virulence. Trans -cinnamaldehyde (TC), the main phytochemical in cinnamon essential oils has demonstrated promising activity against a wide range of pathogens. Here, we comprehensively investigated the effect of TC on planktonic growth, biofilm formation, proteolytic and hemolytic activities, as well as gene regulation in E. faecalis . Our findings revealed that sub-inhibitory concentrations of TC reduced biofilm formation, biofilm exopolysaccharides as well as its proteolytic and hemolytic activities. Mechanistic studies revealed significant down regulation of the quorum sensing fsr locus and downstream gelE , which are major virulence regulators in E. faecalis . Taken together, our study highlights the potential of TC to inhibit E. faecalis biofilm formation and its virulence.

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“…The ATCC 29212 strain was selected as it has been reported to be similar to a clinical isolate (Seneviratne et al 2013). E. faecalis ATCC 29212 has been shown to form a biofilm after 12 h of incubation (Yang et al 2018), and the EPS matrix has demonstrated to cover the biofilm after 6 h (Kristich et al 2004, Ramos et al 2019). In the present study, a 12‐h biofilm was grown trying to create a young biofilm, as it is the biofilm that the residual bacteria would form on dentine walls, after performing chemo‐mechanical cleaning during root canal treatment.…”

Section: Discussionmentioning

confidence: 99%

Doxycycline‐functionalized polymeric nanoparticles inhibit Enterococcus faecalis biofilm formation on dentine

et al. 2020

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Aim To evaluate in a laboratory setting the antimicrobial properties and the potential to inhibit biofilm formation of novel remineralizing polymeric nanoparticles (NPs) when applied to dentine surfaces and to ascertain the effect of the functionalization of these NPs with zinc, calcium or doxycycline. Methodology The antimicrobial activity and inhibition of biofilm formation of polymeric NPs were analysed on human dentine blocks that were infected with Enterococcus faecalis before or after application of NPs. LIVE/DEAD ® testing under Confocal Laser Scanning Microscopy and bacterial culturing were employed to analyse biofilm biovolume and bacterial viability. Field Emission Scanning Electron Microscopy was also employed to assess biofilm morphology. One‐way anova with Welch’s correction and post hoc comparison by the Games–Howell test were performed for comparisons between groups. Results The un‐functionalized NPs displayed the greatest antimicrobial activity against E. faecalis biofilms as they provided the lowest biovolume (3865.7 ± 2926.97 µm3; P < 0.001) and the highest dead/injured cells percentage (79.93 ± 18.40%; P < 0.001), followed by Dox‐NPs (biovolume: 19,041.55 ± 17,638.23 µm3, dead/injured cells: 45.53 ± 26.50%; P < 0.001). Doxycycline‐loaded NPs had the largest values of inhibition of biofilm formation with the lowest biofilm biovolume (8517.65 ± 7055.81 µm3; P < 0.001) and a high dead/injured bacterial percentage (68.68 ± 12.50%; P < 0.001). Un‐functionalized NPs did not reduce biomass growth (P > 0.05), but attained the largest percentage of compromised cells (93 ± 8.23%; P < 0.001), being able to disrupt biofilm formation. It also produced occlusion of dentinal tubules, potentially interfering with bacterial tubule penetration. Conclusions A new generation of bioactive nano‐fillers (doxycycline‐functionalized polymeric NPs) had antibacterial activity and occluded dentinal tubules. Incorporating these NPs into endodontic sealers may have the potential to enhance the outcome of root canal treatment.

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Quorum sensing LuxS/autoinducer-2 inhibits Enterococcus faecalis biofilm formation ability

Cited by 17 publications

References 25 publications

Molecular and Bacteriological Study of Enterococcus faecalis Isolated from Different Clinical Sources

Molecular and Bacteriological Study of Enterococcus faecalis Isolated from Different Clinical Sources

Trans-cinnamaldehyde attenuatesEnterococcus faecalisvirulence and inhibits biofilm formation

Doxycycline‐functionalized polymeric nanoparticles inhibit Enterococcus faecalis biofilm formation on dentine

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