2014
DOI: 10.1371/journal.pone.0084704
|View full text |Cite
|
Sign up to set email alerts
|

Rab27a Is Essential for the Formation of Neutrophil Extracellular Traps (NETs) in Neutrophil-Like Differentiated HL60 Cells

Abstract: Neutrophils play a crucial role in host defence. In response to a variety of inflammatory stimulation, they form neutrophil extracellular traps (NETs). NETs are extracellular structures composed of chromatin fibers decorated with antimicrobial proteins and developing studies indicate that NETs contribute to extracellular microbial killing. While the intracellular signaling pathways that regulate NET formation remain largely unknown, there is growing evidence that generation of reactive oxygen species (ROS) is … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

4
25
1

Year Published

2016
2016
2024
2024

Publication Types

Select...
10

Relationship

0
10

Authors

Journals

citations
Cited by 34 publications
(30 citation statements)
references
References 39 publications
4
25
1
Order By: Relevance
“…In the study by Munafo et al., neutrophils deficient in Rab27a showed normal NET generation. This differs from the data presented in another study, where using HL‐60 cells, Rab27a downregulation is suggested to prevent NET formation . Many different reasons may justify these differences.…”
Section: Degranulation Trafficking Regulators Are Dispensable For Thecontrasting
confidence: 94%
“…In the study by Munafo et al., neutrophils deficient in Rab27a showed normal NET generation. This differs from the data presented in another study, where using HL‐60 cells, Rab27a downregulation is suggested to prevent NET formation . Many different reasons may justify these differences.…”
Section: Degranulation Trafficking Regulators Are Dispensable For Thecontrasting
confidence: 94%
“…HL‐60 cell line, human promyelocytic leukemia cells, can be differentiated into granulocyte‐like cells using a wide variety of agents, such as all‐trans retinoic acid (ATRA), butyrate, hypoxanthine, dimethyl sulfoxide (DMSO) or dimethylformamide (DMF) . Differentiated HL‐60 cells (dHL‐60) have already been applied in studies deciphering mechanisms of NETs formation . It was reported that dHL‐60 cells were able to release NETs in response to a variety of stimuli, including both synthetic (ROS‐dependent phorbol 12‐myristate 13‐acetate (PMA) and ROS‐independent calcium ionophore (CI)) and physiological NETosis inducers ( C. albicans, S. flexneri, S. aureus and interleukin‐8).…”
Section: Introductionmentioning
confidence: 99%
“…Recent reports showed that differentiation of HL-60 cells, a human promyelocytic leukemia cell line, into mature neutrophils with calcium ionophore, ATRA, or dimethyl sulfoxide or exposure to the glycosyltransferase inhibitor tunicamycin (TM) resulted in the formation of NET-like structures. 22, 23, 24, 25 The nuclei of APL blasts lack lobules and the distribution of chromatin is loose, which are precursors to ET release. In this study, we hypothesized that APL cells are able to form extracellular DNA traps (ETs) and explored conditions under which APL cells are activated.…”
mentioning
confidence: 99%