Rab35 – A vesicular traffic‐regulating small GTPase with actin modulating roles

Chua, Christelle En Lin; Lim, Yi Shan; Tang, Bor Luen

doi:10.1016/j.febslet.2009.11.051

Cited by 49 publications

(44 citation statements)

References 58 publications

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“…Furthermore, a possible role of Rab35 in CLIC4-mediated cell adhesion remains to be established. One difficulty with such studies is that (1) the precise function of CLIC4 remains elusive, and (2) Rab35 acts on at least six distinct effectors, consistent with Rab35 mediating numerous processes, and that its activity is regulated by multiple guanine-nucleotide-exchange factors and GTPaseactivating proteins Chua et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

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CLIC4 regulates cell adhesion and β1 integrin trafficking

Argenzio

Margadant

Leyton-Puig

et al. 2014

Journal of Cell Science

100

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BSTRACTChloride intracellular channel protein 4 (CLIC4) exists in both soluble and membrane-associated forms, and is implicated in diverse cellular processes, ranging from ion channel formation to intracellular membrane remodeling. CLIC4 is rapidly recruited to the plasma membrane by lysophosphatidic acid (LPA) and serum, suggesting a possible role for CLIC4 in exocytic-endocytic trafficking. However, the function and subcellular target(s) of CLIC4 remain elusive. Here, we show that in HeLa and MDA-MB-231 cells, CLIC4 knockdown decreases cell-matrix adhesion, cell spreading and integrin signaling, whereas it increases cell motility. LPA stimulates the recruitment of CLIC4 to b1 integrin at the plasma membrane and in Rab35-positive endosomes. CLIC4 is required for both the internalization and the serum-or LPA-induced recycling of b1 integrin, but not for EGF receptor trafficking. Furthermore, we show that CLIC4 suppresses Rab35 activity and antagonizes Rab35-dependent regulation of b1 integrin trafficking. Our results define CLIC4 as a regulator of Rab35 activity and serum-and LPAdependent integrin trafficking.

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Section: Discussionmentioning

confidence: 99%

“…We next examined Rab35, which has recently emerged as a regulator of endocytic recycling (Allaire et al, 2010;Allaire et al, 2013;Chua et al, 2010;Kouranti et al, 2006). In serumstarved HeLa cells, Rab35 was mostly found at the plasma membrane and, to a lesser extent, in intracellular compartments (Fig.…”

Section: Clic4 Is Not Required For Egfr Traffickingmentioning

confidence: 99%

CLIC4 regulates cell adhesion and β1 integrin trafficking

Argenzio

Margadant

Leyton-Puig

et al. 2014

Journal of Cell Science

100

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“…At this point, the cell must proceed to the final cytokinetic event, abscission, leading to the resolution of the ICB. Recent work has demonstrated that remarkable changes occur inside the ICB, such as the reorganization of post-Golgi vesicle trafficking (Chua et al, 2010;Gromley et al, 2005;Wilson et al, 2005) and increases in ICB microtubule dynamics (Connell et al, 2009;Fanarraga et al, 2010). However, the molecular machinery and the mechanisms that mediate abscission during cytokinesis remain to be fully understood.…”

Section: Introductionmentioning

confidence: 99%

Endocytic membrane fusion and buckling-induced microtubule severing mediate cell abscission

Schiel

Park

Morphew

et al. 2011

Journal of Cell Science

105

106

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Cytokinesis and abscission are complicated events that involve changes in membrane transport and cytoskeleton organization. We have used the combination of time-lapse microscopy and correlative high-resolution 3D tomography to analyze the regulation and spatio-temporal remodeling of endosomes and microtubules during abscission. We show that abscission is driven by the formation of a secondary ingression within the intracellular bridge connecting two daughter cells. The initiation and expansion of this secondary ingression requires recycling endosome fusion with the furrow plasma membrane and nested central spindle microtubule severing. These changes in endosome fusion and microtubule reorganization result in increased intracellular bridge plasma membrane dynamics and abscission. Finally, we show that central spindle microtubule reorganization is driven by localized microtubule buckling and breaking, rather than by spastin-dependent severing. Our results provide a new mechanism for mediation and regulation of the abscission step of cytokinesis.

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“…94 The silencing of Rab35 inhibited cell migration in MCF7 breast cancer cells. 95 Conversely, Rab35 mitigates cell migration in brain tumor cells 52 that suggests that Rabs GTPase modulation of cell migration may be tissue specific.…”

Section: 93mentioning

confidence: 99%

The role of endocytic Rab GTPases in regulation of growth factor signaling and the migration and invasion of tumor cells

Porther

Barbieri

2015

Small GTPases

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Metastasis is characterized pathologically by uncontrolled cell invasion, proliferation, migration and angiogenesis. It is a multistep process that encompasses the modulation of membrane permeability and invasion, cell spreading, cell migration and proliferation of the extracellular matrix, increase in cell adhesion molecules and interaction, decrease in cell attachment and induced survival signals and propagation of nutrient supplies (blood vessels). In cancer, a solid tumor cannot expand and spread without a series of synchronized events. Changes in cell adhesion receptor molecules (e.g., integrins, cadherin-catenins) and protease expressions have been linked to tumor invasion and metastasis. It has also been determined that ligand-growth factor receptor interactions have been associated with cancer development and metastasis via the endocytic pathway. Specifically, growth factors, which include IGF-1 and IGF-2 therapy, have been associated with most if not all of the features of metastasis. In this review, we will revisit some of the key findings on perhaps one of the most important hallmarks of cancer metastasis: cell migration and cell invasion and the role of the endocytic pathway in mediating this phenomenon

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Rab35 – A vesicular traffic‐regulating small GTPase with actin modulating roles

Cited by 49 publications

References 58 publications

CLIC4 regulates cell adhesion and β1 integrin trafficking

CLIC4 regulates cell adhesion and β1 integrin trafficking

Endocytic membrane fusion and buckling-induced microtubule severing mediate cell abscission

The role of endocytic Rab GTPases in regulation of growth factor signaling and the migration and invasion of tumor cells

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