Rabbit and pig ear skin sample cryobanking: effects of storage time and temperature of the whole ear extirpated immediately after death

Silvestre, Mattia; Saeed, Ahmed; Cervera, Rita P.; Escribá, Marı́a José; García-Ximénez, F.

doi:10.1016/s0093-691x(02)01185-8

Cited by 38 publications

(27 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nevertheless, in order to enhance cells culture of skin cells deriving from collared peccary, in this study the addition of 20% of FBS was also used to the culture medium, since for some species, this percentage may vary for the same cell type (León-Quinto et al 2011). Moreover, in swine, domestic species phylogenetically nearest to the collared peccary, the serum concentration also varied between 10% and 20% (Silvestre et al 2002, Silvestre et al 2003, Shi et al 2015. Additionally, a serum concentration greater than 10% may be useful in situations where the number of fragments is reduced.…”

Section: Discussionmentioning

confidence: 99%

In vitro culture of somatic cells derived from ear tissue of collared peccary (Pecari tajacu Linnaeus, 1758) in medium with different requirements

et al. 2016

View full text Add to dashboard Cite

The maintenance of metabolic activities during the in vitro culture of somatic cells of wild animals, especially collared peccary (Pecari tajacu), is an interesting step in conservation of these cells for the use in nuclear transfer. In this context, it is necessary to optimize the culture conditions of somatic cells by the establishment of appropriate supplementation to the media. Therefore, this study aimed to analyze the composition of the culture means of somatic cell derived from ear tissue of collared peccaries, evaluating concentrations of fetal bovine serum (FBS; 10% vs. 20%) and epidermal growth factor (EGF; 5ng/mL vs. 10ng/mL). Tissues were submitted to primary culture and subcultures for 40 days and cells were analyzed for morphology, adhesion, subconfluence, and proliferative activity to develop the growth curve and to determine the population doubling time (PDT), viability, and functional/metabolic activity. No difference was observed between the concentrations of FBS for several parameters, except for viability [FBS10: 85.6% vs. FBS20: 98.2%], PDT [FBS10: 155.4h vs. 77.2h], and functional/metabolic assay [FBS10: 0.57-0.55 vs. FBS20: 0.82-0.99 (D5-D7)]. For the EGF in culture, no difference was observed in the evaluated parameters. In all experiments, the growth curves were typical S-shape and the cells passed through a lag, logarithmic, and plateau phase. In conclusion, 20% FBS is suitable for the recovery of somatic cells; nevertheless, EGF does not improve the quality of growing these cells. To our knowledge, this is the first study culturing somatic cells of collared peccaries.

show abstract

Section: Discussionmentioning

confidence: 99%

In vitro culture of somatic cells derived from ear tissue of collared peccary (Pecari tajacu Linnaeus, 1758) in medium with different requirements

et al. 2016

View full text Add to dashboard Cite

show abstract

“…[10][11][12] However, they worked postmortem, while skin samples were placed in straws and seeded directly in a plastic dish to obtain fibroblasts. In contrast, we worked with anesthetized wild animals, used cryovials to facilitate the handling, and fibroblasts were obtained after using a collagenase treatment.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of Brown Bear Skin Biopsies

Caamaño

Rodríguez

Muñoz

et al. 2008

Cell Preservation Technology

View full text Add to dashboard Cite

Genetic resource banks and assisted reproductive technologies support the conservation of endangered or threatened species. In this study we assessed two procedures to cryopreserve skin biopsies from live brown bears. Skin biopsies were taken from six live, anesthetized brown bears. Single biopsies (n ‫؍‬ 3) of each animal were cut into small pieces and assigned to one of the three experimental groups: freezing, vitrification, or untreated fresh. There were no differences on cell attachment. However, both freezing and fresh culture allowed for higher cell proliferation (p Ͻ 0.05) and less days to reach 70% to 80% confluence (p Ͻ 0.03) than vitrification. Skin biopsies from brown bears can be preserved long term, allowing fibroblasts to proliferate in culture. Slow freezing was effective to cryopreserve skin biopsies from brown bears. 83

show abstract

“…Inverse correlation between skin viability and storage temperature belongs to the basic facts about skin graft preservation [1,[13][14][15][16]. The current study attempted to use the detrimental effects of heat to shorten the duration of experimental skin storage.…”

Section: Discussionmentioning

confidence: 99%

“…According to the storage times (0,7,8,9,10,11,12,13,14,15,16,17, or 18 min), there were 12 subgroups in the NaCl-F group (from 0 to 17, denoted as NaCl-F-0, NaCl-F-7, NaCl-F-8, etc.) and 13 subgroups in each male group (from 0 to 18, denoted in the above fashion).…”

Section: Study Groupsmentioning

confidence: 99%

“…This pilot study was prompted by two problems in skin preservation research: (1) due to relatively long storage periods at low temperatures, evaluation of storage conditions requires lengthy experimentation [1][2][3][4][5][6][7][8][9][10], and (2) due to partial necrosis, it is difficult to assess survival of stored skin grafts in vivo [8,11,12]. The principal aim of the current investigation was to find out whether these problems can be potentially solved by using experimental approaches based on the knowledge that (1) raising temperature reduces the length of useful skin preservation [1,[13][14][15][16], and (2) if implanted subcutaneously, viable skin grafts result in epithelial cysts [17][18][19], while the nonviable ones are resorbed [18]. For this purpose, a novel experimental model was employed.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Skin Preservation at a Supraphysiologic Temperature and Induction of Epithelial Cysts by Subcutaneous Grafting: Attempts to Simplify Evaluation of Storage Media and of Graft Viability

Macionis¹

2004

Eur Surg Res

View full text Add to dashboard Cite

This pilot study used a new experimental rat model designed to explore whether in-depth investigation of the following two questions would be reasonable: (1) Can skin storage media be faster evaluated by storing skin at a supraphysiologic temperature, and (2) can evaluation of skin graft viability be facilitated by subcutaneous grafting leading to production of epithelial cysts from the viable portions of the grafts? The experiment involved skin storage at 47.3°C in saline and in 10% Dextran 40 solution for periods ranging from 0 to 18 min, followed by subcutaneous grafting. The surface area of the resultant epithelial cysts and the cyst-to-graft ratio inversely correlated with storage time, which is in accordance with a well-established analogous relationship involving the rate of graft ‘take’. The skin tended to remain viable longer in Dextran 40 solution, which is consistent with previous studies of low temperature storage demonstrating superiority of high-molecular-weight solutions. The results warrant further investigation of the above issues.

show abstract

Rabbit and pig ear skin sample cryobanking: effects of storage time and temperature of the whole ear extirpated immediately after death

Cited by 38 publications

References 22 publications

In vitro culture of somatic cells derived from ear tissue of collared peccary (Pecari tajacu Linnaeus, 1758) in medium with different requirements

In vitro culture of somatic cells derived from ear tissue of collared peccary (Pecari tajacu Linnaeus, 1758) in medium with different requirements

Cryopreservation of Brown Bear Skin Biopsies

Skin Preservation at a Supraphysiologic Temperature and Induction of Epithelial Cysts by Subcutaneous Grafting: Attempts to Simplify Evaluation of Storage Media and of Graft Viability

Contact Info

Product

Resources

About