Rabbit M1 and M2 macrophages can be induced by human recombinant <scp>GM</scp>‐<scp>CSF</scp> and M‐<scp>CSF</scp>

Yamane, Kazuyoshi; Leung, Kai P.

doi:10.1002/2211-5463.12101

Cited by 17 publications

(13 citation statements)

References 33 publications

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“…41 Median IL-1β mRNA was 34% lower in the group of HDAdApoAI-treated arteries than the group of HDAdNull-infused arteries; median MRC1 mRNA was 7% higher in HDAdApoAI-treated arteries. When compared to their paired HDAdNull-treated controls, HDAdApoAI-treated arteries had 14% lower median IL-1β mRNA expression ( P =0.4) and 8% higher median MRC1 mRNA expression ( P =0.4).…”

Section: Resultsmentioning

confidence: 80%

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Local Vascular Gene Therapy With Apolipoprotein A-I to Promote Regression of Atherosclerosis

Wacker

Dronadula

Zhang

et al. 2017

ATVB

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Objective Gene therapy, delivered directly to the blood vessel wall, could potentially prevent atherosclerotic lesion growth and promote atherosclerosis regression. Previously, we reported that a helper-dependent adenoviral vector (HDAd) expressing apolipoprotein (apo) A-I in carotid endothelium of fat-fed rabbits reduced early (4 weeks) atherosclerotic lesion growth. Here we tested whether the same HDAd—delivered to existing carotid atherosclerotic lesions—could promote regression. Approach and Results Rabbits (n=26) were fed a high-fat diet for 7 months, then treated with bilateral carotid gene transfer. One carotid was infused with an HDAd expressing apo A-I (HDAdApoAI), the other with a control non-expressing HDAd (HDAdNull). The side with HDAdApoAI was randomized. Rabbits were then switched to regular chow, lowering their plasma cholesterols by over 70%. ApoA-I mRNA and protein were detected in HDAdApoAI-transduced arteries. After 7 weeks of gene therapy, compared to HDAdNull-treated arteries in the same rabbits, HDAdApoAI-treated arteries had significantly less VCAM-1 expression (28%; P=0.04) along with modest but statistically insignificant trends towards decreased intimal lesion volume, lipid and macrophage content, and ICAM-1 expression (9%–21%; P=0.1–0.4). Post-hoc subgroup analysis of rabbits with small-to-moderate-sized lesions (n=20) showed that HDAdApoAI caused large reductions in lesion volume, lipid content, ICAM-1 and VCAM-1 expression (30%–50%; P≤0.04 for all). Macrophage content was reduced by 30% (P=0.06). There was a significant interaction (P=0.02) between lesion size and treatment efficacy. Conclusion Even when administered on a background of aggressive lowering of plasma cholesterol, local HDAdApoAI vascular gene therapy may promote rapid regression of small-to-moderate-sized atherosclerotic lesions.

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Section: Resultsmentioning

confidence: 80%

“…When compared to their paired HDAdNull-treated controls, HDAdApoAI-treated arteries had 14% lower median IL-1β mRNA expression ( P =0.4) and 8% higher median MRC1 mRNA expression ( P =0.4). Attempts to measure several other proposed rabbit M1 and M2 markers by qRT-PCR 41 were not successful.…”

Section: Resultsmentioning

confidence: 99%

Local Vascular Gene Therapy With Apolipoprotein A-I to Promote Regression of Atherosclerosis

Wacker

Dronadula

Zhang

et al. 2017

ATVB

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“…First, we checked the macrophage polarization paradigm in rabbits by stimulating blood-derived macrophages with cytokines inducing the M1 (IFN-γ) or the M2 (IL-4) profiles. Rabbit macrophages have been polarized into M1 and M2 by stimulation with human GM-CSF or M-CSF, respectively [9]. Relative expression of biological markers representative of each maturation state points out for a conserved mechanism among humans, mice, sheep and rabbits generalizing M1 and M2 differentiation pathways.…”

Section: Discussionmentioning

confidence: 99%

“…M1, or classically activated macrophages, produce high levels of pro-inflammatory cytokines and M2, or alternatively activated macrophages, show an anti-inflammatory profile involved in wound healing and tissue repair [6,7]. Molecules differentially expressed in each macrophage subpopulation can be employed as markers of a specific differentiation pathway [8] also in rabbits [9]. …”

Section: Introductionmentioning

confidence: 99%

Lentinula edodes β-glucan enriched diet induces pro- and anti-inflammatory macrophages in rabbit

Crespo

Guillén²,

Pablo-Maiso

et al. 2017

Food & Nutrition Research

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ABSTRACTβ-glucans exhibited in cell walls of several pathogens as bacteria or fungi are sensed by pathogen recognition receptors such as scavenger receptors present in antigen presenting cells, i.e., macrophages. β-glucans obtained from Shiitake mushrooms were chemically characterized. A β-glucan supplemented diet was assayed for 30 days in rabbits aiming to characterize the immune response elicited in blood-derived macrophages. M1 and M2 profiles of macrophage differentiation were confirmed in rabbits by in vitro stimulation with IFN-γ and IL-4 and marker quantification of each differentiation pathway. Blood derived macrophages from rabbits administered in vivo with the β-glucan supplemented diet showed higher IL-4, IFN-γ and RAGE together with lower IL-10 relative expression, indicative of an ongoing immune response. Differences in IL-1β, IL-13 and IL-4 expression were also found in rabbit sera by ELISA suggesting further stimulation of the adaptive response. Recent challenges in the rabbit industry include the search of diet supplements able to elicit an immune stimulation with particular interest in facing pathogens such as viruses or bacteria. β–glucans from fungi may contribute to maintain an immune steady state favouring protection and thus reducing antibiotic treatment.

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“…Their metabolism is activated toward the production of high levels of pro-inflammatory cytokines such as IFN-γ, IL-1, IL-6, and TNF-α (Schairer et al, 2012;Novak and Koh, 2013). Typically, M1 macrophages present augmented levels of major histocompatibility complex (MHC) cell surface receptors, known as HLA-DR, and the chemokine receptors CD197 that bind CCL19 and CCL21 (Yamane and Leung, 2016).…”

Section: Introductionmentioning

confidence: 99%

Response of Human Macrophages to Clinically Applied Wound Dressings Loaded With Silver

Varela

Marlinghaus

Sartori

et al. 2020

Front. Bioeng. Biotechnol.

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Wound infections constitute an increasing clinical problem worldwide. To reverse this trend, several wound dressings with antimicrobial properties have been developed. Considering the increasing presence of antibiotic-resistant microorganisms, product developers have been focusing their efforts in introducing antibiotic-free antibacterial wound dressings to the market, with silver being the most commonly incorporated antimicrobial agent. In this scenario, gaining information about the microbial and eukaryotic cells' response to these dressings is needed for a proper selection of antimicrobial dressings for the different cases of infected wounds. In particular, one insufficiently explored parameter is the effect of the dressings on the immunomodulation of macrophages, the main immune cell population participating in the repair process, because of their pivotal role in the transition of the inflammation to the proliferation phase of wound healing. In this work, three different clinically applied antimicrobial, silver impregnated wound dressings were selected: Atrauman R Ag, Biatain R Alginate Ag and PolyMem WIC Silver R Non-adhesive. Antimicrobial susceptibility tests (disk diffusion and broth dilution), cell viability evaluation (CellTiter-Blue R) and experiments to determine macrophage polarization (e.g., flow cytometry, ELISA and glucose uptake) were performed after 24 h of incubation. Among all products tested, Biatain R Alginate Ag induced the most evident bactericidal effect on Gram-positive and Gramnegative bacteria, followed by PolyMem WIC Silver R Non-adhesive, but did not show good cytocompatibility in vitro. On the other hand, Atrauman R Ag showed excellent cytocompatibility on L929 fibroblasts, HaCaT keratinocytes and THP-1 derived macrophages, but no significant antimicrobial activity was observed. Overall, it was confirmed that macrophages initiate, in fact, an alteration of their metabolism and phenotype in response to wound dressings of different composition in a short period of contact (24 h). M0 resting state macrophages common response to all silver-containing dressings used in this study was to increase the production of the anti-inflammatory cytokine TGF-β, which indicates an acquisition of M2-like macrophages characteristics.

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Rabbit M1 and M2 macrophages can be induced by human recombinant GM‐CSF and M‐CSF

Cited by 17 publications

References 33 publications

Local Vascular Gene Therapy With Apolipoprotein A-I to Promote Regression of Atherosclerosis

Local Vascular Gene Therapy With Apolipoprotein A-I to Promote Regression of Atherosclerosis

Lentinula edodes β-glucan enriched diet induces pro- and anti-inflammatory macrophages in rabbit

Response of Human Macrophages to Clinically Applied Wound Dressings Loaded With Silver

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