Radiation dose rate affects the radiosensitization of MCF-7 and HeLa cell lines to X-rays induced by dextran-coated iron oxide nanoparticles

Khoshgard, Karim; Kiani, Parvaneh; Haghparast, Abbas; Hosseinzadeh, Leila; Eivazi, Mohammad Taghi

doi:10.1080/09553002.2017.1321806

Cited by 24 publications

(11 citation statements)

References 23 publications

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“…The most significant effects were seen in MCF7 and HEPG2 cells (P < 0.0001). These data are in contrast to a previous study by Khoshgard et al which reported no cytotoxicity for SPIONs following 24 h exposures in MCF7 and HeLa cells, using an MTT assay [67]. Also, the inherent cytotoxicity observed in our study did not correlate DNA damage, suggesting that it is possible that in cell lines other than U87 and HEPG2, any decrease in cell survival post-irradiation was due to radiosensitisation, along with other possible causes of DNA damage.…”

Section: Discussioncontrasting

confidence: 99%

Impact of Superparamagnetic Iron Oxide Nanoparticles on In Vitro and In Vivo Radiosensitisation of Cancer Cells

Russell

Dunne

Russell

et al. 2020

Preprint

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PurposeThe recent implementation of MR-Linacs has highlighted theranostic opportunities of contrast agents in both imaging and radiotherapy. There is a lack of data exploring the potential of superparamagnetic iron oxide nanoparticles (SPIONs) as radiosensitisers. This study aimed to characterise the uptake and radiobiological effects of SPIONs in tumour cell models in vitro and to provide proof-of-principle application in a xenograft tumour model. MethodsSPION uptake was measured using ICP-MS in 6 cancer cell lines; H460, MiaPaCa2, DU145, MCF7, U87 and HEPG2. The impact of SPIONs on radiobiological response was determined by measuring DNA damage using 53BP1 immunofluorescence and cell survival. Measured dose enhancement factors (DEFs) were with the predicted DEFs based on physical absorption estimations. In vivo efficacy was demonstrated using a subcutaneous H460 xenograft tumour model in SCID mice by following intra-tumoural injection of SPIONs. ResultsSPIONs significantly increased DNA damage in all cell lines with the exception of U87 cells at a dose of 1 Gy, 1 hr post-irradiation. Levels of DNA damage correlated with the cell survival, in which all cell lines except U87 cells showed an increased sensitivity (P < 0.05) in the linear quadratic curve fit for 1 hr exposure to 0.1 mM SPIONs. There was also a 30.1 % increase in the number of DNA damage foci found for HEPG2 cells at 2 Gy. No strong correlation was found between SPION uptake and DNA damage at any dose, yet the biological consequences of SPIONs on radiosensitisation were found to be much greater, with DEFs up to 1.28 ± 0.03, compared with predicted physical dose enhancement levels of 1.0001. In vivo, intra-tumoural injection of SPIONs combined with radiation showed significant tumour growth delay compared to animals treated with radiation or SPIONs alone (p < 0.05). ConclusionsSPIONs showed radiosensitising effects in 5 out of 6 cancer cell lines. No correlation was found between the cell-specific uptake of SPIONs into the cells and DNA damage levels. The in vivo study found a significant decrease in the tumour growth rate.

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Section: Discussioncontrasting

confidence: 99%

Impact of Superparamagnetic Iron Oxide Nanoparticles on In Vitro and In Vivo Radiosensitisation of Cancer Cells

Russell

Dunne

Russell

et al. 2020

Preprint

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“…The most significant effects were seen in MCF7 and HEPG2 cells (P < 0.0001). These data are in contrast to a previous study by Khoshgard et al which reported no cytotoxicity for SPIONs following 24 hr exposures in MCF7 and HeLa cells, using an MTT assay(60). Also, the inherent cytotoxicity observed in our study did not correlate DNA damage, suggesting that in cell lines other than U87 and HEPG2, any decrease in cell survival post-irradiation was due to radiosensitisationThe impact of combinations of SPIONs with radiation on DNA damage yields is presented inFigure 3.…”

contrasting

confidence: 99%

Impact of Superparamagnetic Iron Oxide Nanoparticles on In Vitro and In Vivo Radiosensitisation of Cancer Cells

Russell

Dunne

Russell

et al. 2020

Preprint

View full text Add to dashboard Cite

Purpose The recent implementation of MR-Linacs has highlighted theranostic opportunities of contrast agents in both imaging and radiotherapy. There is a lack of data exploring the potential of superparamagnetic iron oxide nanoparticles (SPIONs) as radiosensitisers. This study aimed to characterise the uptake and radiobiological effects of SPIONs in tumour cell models in vitro and to provide proof-of-principle application in a xenograft tumour model. Methods SPION uptake was measured using ICP-MS in 6 cancer cell lines; H460, MiaPaCa2, DU145, MCF7, U87 and HEPG2. The impact of SPIONs on radiobiological response was determined by measuring DNA damage using 53BP1 immunofluorescence and cell survival. Measured dose enhancement factors (DEFs) were with the predicted DEFs based on physical absorption estimations. In vivo efficacy was demonstrated using a subcutaneous H460 xenograft tumour model in SCID mice by following intra-tumoural injection of SPIONs. Results SPIONs significantly increased DNA damage in all cell lines with the exception of U87 cells at a dose of 1 Gy, 1 hr post-irradiation. Levels of DNA damage correlated with the cell survival, in which all cell lines except U87 cells showed an increased sensitivity (P < 0.05) in the linear quadratic curve fit for 1 hr exposure to 0.1 mM SPIONs. There was also a 30.1 % increase in the number of DNA damage foci found for HEPG2 cells at 2 Gy. No strong correlation was found between SPION uptake and DNA damage at any dose, yet the biological consequences of SPIONs on radiosensitisation were found to be much greater, with DEFs up to 1.28 ± 0.03, compared with predicted physical dose enhancement levels of 1.0001. In vivo , intra-tumoural injection of SPIONs combined with radiation showed significant tumour growth delay compared to animals treated with radiation or SPIONs alone (p < 0.05). Conclusions SPIONs showed radiosensitising effects in 5 out of 6 cancer cell lines. No correlation was found between the cell-specific uptake of SPIONs into the cells and DNA damage levels. The in vivo study found a significant decrease in the tumour growth rate.

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“…Cell survival curves from Figure7to 10 showed that MCF-7 cells experienced more death compared to HeLa cells when irradiated with photon and electron beam. These results indicated that the Bi 2 O 3 -NR influenced radiosensitization more in MCF-7 cells Khoshgard (2017). reported that dextran-coated iron oxide nanoparticles increase the effect of radiosensitivity when irradiated with a 6 MV photon beam.…”

mentioning

confidence: 77%

“…reported that dextran-coated iron oxide nanoparticles increase the effect of radiosensitivity when irradiated with a 6 MV photon beam. It was discovered from the study that MCF-7 cells produced higher SER values (1.21 ± 0.06) compared to HeLa cells (1.19 ± 0.04)[52]. The difference is presumably due to the different characteristics of the cells such as type of receptors, number of mitochondria, chromatin structure, all of which affect radiation sensitivity.Mitochondria play a role in regulating the oxidative stress level inside cells.…”

mentioning

confidence: 97%

Radiosensitization effects by bismuth oxide nanorods of different sizes in megavoltage external beam radiotherapy

Jamil

Abidin

Razak

et al. 2021

Rep Pract Oncol Radiother.

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Radiation dose rate affects the radiosensitization of MCF-7 and HeLa cell lines to X-rays induced by dextran-coated iron oxide nanoparticles

Abstract: Increasing the dose rate of 6 MV photons irradiation in MCF-7 and HeLa cells increases the radiosensitization induced by the dextran-coated iron nanoparticles in these cells.

Cited by 24 publications

References 23 publications

Impact of Superparamagnetic Iron Oxide Nanoparticles on In Vitro and In Vivo Radiosensitisation of Cancer Cells

Impact of Superparamagnetic Iron Oxide Nanoparticles on In Vitro and In Vivo Radiosensitisation of Cancer Cells

Impact of Superparamagnetic Iron Oxide Nanoparticles on In Vitro and In Vivo Radiosensitisation of Cancer Cells

Radiosensitization effects by bismuth oxide nanorods of different sizes in megavoltage external beam radiotherapy

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