Radiation synthesis and characterization of protein stabilized gold nanoparticles

Akhavan, Azam; Kalhor, Hamid Reza; Kassaee, M.Z.; Sheikh, N.; Hassanlou, Maryam

doi:10.1016/j.cej.2010.02.010

Cited by 46 publications

(26 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The stability of colloidal AuNPs solution depends on various factors such as pH, dielectric constant, and concentration of ligand around the particles [19]. Furthermore, the stability of colloidal AuNPs solution stored at low temperature is better than at high ones [40].…”

Section: Characteristics Of Aunps Powdersmentioning

confidence: 99%

“…In addition, biopolymers such as chitosan [9][10][11], alginate [12,13], hyaluronan [14,15], gum arabic [16,17], protein [18,19], gelatin [20], dextran [21], and glucomannan [22] have been also used as stabilizers of colloidal AuNPs solution. On the other hand, materials based on functionalized AuNPs are regarded as one of the most important nanocomposites especially due to their biological and catalytic activities [23,24].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Synthesis of Gold Nanoparticles Stabilized in Dextran Solution by Gamma Co-60 Ray Irradiation and Preparation of Gold Nanoparticles/Dextran Powder

Diem

Thao

Phú

et al. 2017

Journal of Chemistry

View full text Add to dashboard Cite

Gold nanoparticles (AuNPs) in spherical shape with diameter of 6-35 nm stabilized by dextran were synthesized by -irradiation method. The AuNPs were characterized by UV-Vis spectroscopy and transmission electron microscopy. The influence of pH, Au 3+ concentration, and dextran concentration on the size of AuNPs was investigated. Results indicated that the smallest AuNPs size (6 nm) and the largest AuNPs size (35 nm) were obtained for pH of 1 mM Au 3+ /1% dextran solution of 5.5 and 7.5, respectively. The smaller Au 3+ concentration favored smaller size and conversely the smaller dextran concentration favored bigger size of AuNPs. AuNPs powders were prepared by spay drying, coagulation, and centrifugation and their sizes were also evaluated. The purity of prepared AuNPs powders was also examined by energy dispersive X-ray (EDX) analysis. Thus, the as-prepared AuNPs stabilized by biocompatible dextran in solution and/or in powder form can be potentially applied in biomedicine and pharmaceutics.

show abstract

Section: Characteristics Of Aunps Powdersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synthesis of Gold Nanoparticles Stabilized in Dextran Solution by Gamma Co-60 Ray Irradiation and Preparation of Gold Nanoparticles/Dextran Powder

Diem

Thao

Phú

et al. 2017

Journal of Chemistry

View full text Add to dashboard Cite

show abstract

“…According to the data of SDS-PAGE (dodecyl sulfate polyacrylamide gel electrophoresis), polypeptide chain breaks down at doses up to 1 kGy, and at 5 kGy or higher fragments of the destroyed protein begin to form large molecular weight aggregates [29]. Specifically, doses up to 0.2 kGy damage 10-20% of BSA [30]; at 1 kGy, 50-60% of BSA is damaged [30], and even at 2.5 kGy some amount of undamaged BSA still remains [31]. In our case, the ionizing radiation changes the number of zigzag segments, but the average length L mean , angles (α mean ) and other distribution parameters are practically independent of the dose.…”

Section: Gamma Irradiation Of Bsa Solutions Bymentioning

confidence: 99%

Characterization of zigzag patterns on the surface of bovine serum albumin films

Glibitskiy¹,

Gorobchenko²,

Ніколов³

et al. 2017

Biophysical Bulletin

View full text Add to dashboard Cite

Analysis of the patterns formed during the drying of biological fluids is employed for research and diagnosis in medicine and agriculture. Saline solutions of native proteins and DNA are characterized by zigzag patterns, which could be quantitatively described using the specific length of zigzags L specific . The aim of this study was to analyze a wide number of characteristics in order to identify those most sensitive to the effects of various physical and chemical factors on the protein structure. We examined the films prepared from salt solutions of bovine serum albumin (BSA) under varying conditions, viz.: a proportional change in the concentration of the components, substitution of NaCl with NaF and NaBr, temperature treatment, gamma irradiation, and addition of trivalent iron and biologically active flavin mononucleotide. The results revealed that the distribution of zigzag segment lengths was approximately lognormal, and the distribution of angles between the segments was approximately logistic. Two parameters appeared to be the most informative, viz. the average length of the segments, mainly depending on Cl -concentration, and the number of segments, depending on: Cl -concentration, concentration of the non-aggregated (but not necessarily unfragmented) protein, and the excess concentration of ions and low molecular weight compounds. KEY WORDS: BSA, film, zigzag patterns, texture, statistical distribution. Аналіз патернів, що формуються при висиханні біологічних рідин, використовується для досліджень і діагностики в медицині і сільському господарстві. Для сольових розчинів нативних білків і ДНК характерні зигзагоподібні патерни, які можна кількісно описати за допомогою питомої довжини зигзагів L specific . У даній роботі ми аналізуємо розширений ряд характеристик цих патернів з метою виявити найбільш чутливі до дії різних фізичних і хімічних факторів на структуру білка. Були розглянуті плівки, отримані з сольових розчинів бичачого сироваткого альбуміну (БСА) при пропорційній зміні концентрації компонентів, заміні NaCl на NaF і NaBr, температурній обробці, гамма-опроміненні, а також додаванні тривалентного заліза і біологічно активної речовини -флавінмононуклеотида. Було визначено, що розподіл довжин сегментів зигзагів має близький до логнормального характер, а розподіл кутів між сегментами -близький до логістичного. Дані показали, що найбільш інформативними є два параметри: середня довжина сегментів, яка переважно залежить від концентрації Cl -, і кількість сегментів, яка залежить від концентрації Cl -, концентрації неагрегованого білка (але не обов'язково нефрагментованого), а також від надлишкової концентрації іонів і низькомолекулярних сполук. АНАЛІЗ ХАРАКТЕРИСТИК ЗИГЗАГОПОДІБНИХ ПАТЕРНІВКЛЮЧОВІ СЛОВА: БСА, плівка, зигзагоподібні патерни, текстура, статистичний розподіл.

show abstract

“…[29] AuNPs are also synthesized by means of photochemical reduction method involving HAuCl 4 and aqueous glycine solution exposed to UV irradiation. [30,31] Microwave irradiation method was embraced to synthesize AuNPs by using citric acid as a reducing agent and cetyltrimethyl ammonium bromide (CTAB) as a binding agent.…”

Section: World Journal Of Pharmaceutical Researchmentioning

confidence: 99%

Evolution of Gold Nanoparticles: Pharmacokinetics, Toxicology and Patent Overview.

Shaji¹

2017

WJPR

View full text Add to dashboard Cite

show abstract

Radiation synthesis and characterization of protein stabilized gold nanoparticles

Cited by 46 publications

References 29 publications

Synthesis of Gold Nanoparticles Stabilized in Dextran Solution by Gamma Co-60 Ray Irradiation and Preparation of Gold Nanoparticles/Dextran Powder

Synthesis of Gold Nanoparticles Stabilized in Dextran Solution by Gamma Co-60 Ray Irradiation and Preparation of Gold Nanoparticles/Dextran Powder

Characterization of zigzag patterns on the surface of bovine serum albumin films

Evolution of Gold Nanoparticles: Pharmacokinetics, Toxicology and Patent Overview.

Contact Info

Product

Resources

About