Radiative-surface plasmon resonance for the detection of apolipoprotein E in medical diagnostics applications

Sciacca, Beniamino; François, Alexandre; Klingler‐Hoffmann, Manuela; Brazzatti, Julie; Penno, Megan A. S.; Hoffmann, Peter; Monro, Tanya M.

doi:10.1016/j.nano.2012.10.007

Cited by 50 publications

(27 citation statements)

References 27 publications

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“…Application of a neutravidin solution onto the bare, i.e., unmodified, sensor surface for about 10 minutes led to a shift in resonance wavelength of about 500-600 pm ( Figure 13). A full monolayer of neutravidin has been reported to be roughly equivalent with a layer thickness of 3.5 nm [27], which is equivalent with a shift of about 800 pm, considering a surface sensitivity of 229 pm shift per nm layer thickness (Equation 3). The observed shift therefore is assumed to represent about 60-75% of a packed monolayer of neutravidin.…”

Section: Resultsmentioning

confidence: 99%

Performance of Arrayed Microring Resonator Sensors with the TriPleX Platform

GAJ¹,

RG²

2016

J Biosens Bioelectron

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Integrated-optical (bio) chemical sensors are more and more commercialized, mainly because of their high intrinsic sensitivity in combination with the possibilities they offer for integration in optofluidic devices. The microring resonator (MRR), for example, is a very feasible structure to be used as microarray sensor element for multiplex biosensing. Fabrication of MRRs has been described in the case of the TriPleX platform employing well-defined stacks of stoichiometric Si 3 N 4 and SiO 2 material. This platform allows for a wide variety of applications, due to its intrinsic low loss and its transparency for the VIS/NIR wavelength range. In the present paper, we describe the recent achievements in the characterization of arrays of these MRRs regarding temperature sensitivity, refractive index sensitivity and protein immobilization performance. Furthermore, the use of a reference channel/reference MRR is demonstrated in order to show the advantage of compensation of unintended change in temperature or sample composition. The refractive index sensitivity was determined to be 104 nm/RIU and the limit of detection was about 2 × 10 -6 RIU. MRRs appeared to behave very comparable (expressed as the coefficient of (intra-array) variation (CV)) regarding the response to temperature (CV≈0.3%) as well as refractive index (CV<0.1%). Furthermore, it is shown that protein immobilization onto the different MRRs of the same arrays can be realized in a comparable way (CV<3%). A good comparability (i.e., a low variation) in these aspects allows for the use of internal referencing in order to compensate for unintended change in temperature or refractive index and also helps to attain a high assay precision in the ultimate biosensing application. Ongoing research is focused on the integration of more functionality on chip and further miniaturization in order to allow for fabrication of complex, though affordable, sensor devices.

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Section: Resultsmentioning

confidence: 99%

Performance of Arrayed Microring Resonator Sensors with the TriPleX Platform

GAJ¹,

RG²

2016

J Biosens Bioelectron

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“…A white light LED (Thorlabs Inc., MCWHL2) was used for SPR analysis. A presence of significant surface roughness of the metal coating allowed for analysis of light scattered by surface plasmons directly above the sensor surface [25][26][27] instead of transmission measurements as it is done in conventional SPR fibre sensors [33]. PE layer deposition and dye attachment on the fiber samples were performed inside the flow cell and the SPR response was recorded at each step of the functionalization process.…”

Section: Methodsmentioning

confidence: 99%

“…Based on the determined optimal surface roughness and the spacing between the fluorophore and the metal film, we demonstrate the fabrication of MEF based sensor on a side of a multimode optical fiber. It was previously reported that a fiber configuration with a rough silver coating could be used for fabrication of radiative Surface Plasmon Resonance (SPR) sensors that exploit scattering of the plasmonic wave [25][26][27]. Building on this SPR fiber sensing platform, we study the dependence of fluorescence intensity on the thickness of the silver films and discuss advantages and limitations of the MEF fiber sensor.…”

Section: Introductionmentioning

confidence: 99%

Effect of surface roughness on metal enhanced fluorescence in planar substrates and optical fibers

Klantsataya¹,

François²,

Ebendorff-Heidepriem³

et al. 2016

Opt. Mater. Express

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Abstract:We investigate the effect of the roughness of thin silver films on the performance of sensors that exploit metal enhanced fluorescence (MEF). Fluorescence enhancement of dye molecules of up to 47 times was observed on planar glass substrates coated with metal films of higher roughness of around 8 nm. We also study the fluorescence enhancement on the rough silver films implemented on a side of an optical fiber and analyze its dependence on the thickness of the metal. A maximum enhancement factor of 15 was demonstrated for thinner coatings where the film could be considered as a layer of particles. The chemical electroless plating technique used here to produce films with desired roughness is a low cost simple alternative to complex procedures that are currently used for fabrication of nanostructured metal coatings on optical fibers for MEF.

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“…For such applications it is necessary to immobilize sensor molecules on the glass surface of the MOF exposed core, which can then be used directly within the medium as a sensor without requiring prior pre-mixing of analyte with sensor molecules. Functionalization methods traditionally used include silanes [7,8] or polyelectrolytes [9,10], which provide a functional group on the surface to which the sensor molecules can be covalently attached. These processes require several steps that typically take many hours to perform, due to the incubation times needed to achieve consistent maximized binding efficiency [8,10].…”

Section: Introductionmentioning

confidence: 99%