Radical Scavenging Activity and Preliminary Phytochemical Screening of Pods of <i>Cassia arereh </i> Del. (Fabaceae)

Abstract

Cassia arereh is traditionally used as a fish poison and to manage different disease conditions including gastrointestinal tract (GIT) disorders, infertility, diabetes, insect bite, and infections. Free radicals have been implicated in the pathogenesis of a range of chronic diseases; and many medicinal plants are thought to be effective in managing such diseases, mainly through their free radicals scavenging ability. The objective of this study was to conduct phytochemical screening and investigate the free radical scavenging activity of various extracts of pods of C. arereh. Petroleum ether, ethanol, and water extracts were prepared from the pods powder and tested for their radical scavenging activity using 1, 1-Diphenyl-2-picrylhydrazyl (DPPH) assay. The pods powder was also subjected to preliminary phytochemical screening. The results revealed that ethanol and water extracts possessed strong DPPH radical scavenging activity with 50% inhibitory concentration (IC 50 ) values of 8.84 and 16.76μg/ml, respectively. Ascorbic acid was used as a standard and exhibited a radical scavenging IC 50 value of 2.0µg/ml. Results of preliminary phytochemical screening indicated the possible presence of anthraquinones, carbohydrates, deoxy-sugars, saponins, tannins, and terpenoids. It can be concluded that pods of C. arereh may contain medicinally relevant constituents such as terpenoids and displayed strong radical scavenging activity, which may partly contribute to the possible scientific basis for its traditional use to alleviate different disease conditions.