Radicals from “Good's” buffers

Grady, John K.; Chasteen, N. Dennis; Harris, Daniel C.

doi:10.1016/0003-2697(88)90167-4

Cited by 128 publications

(91 citation statements)

References 20 publications

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“…The exaggerated effect of the two together multiplies the potential problems. The findings with Hepes are likely to apply to other ' Good ' buffers equipped with a piperazine ring, such as Mops, Pipes and EPPS [4-(2-hydroxyethyl)-1-piperazinepropanesulphonic acid] [25,39], and we suggest that they should be only used with caution, particularly in experiments with NO.…”

Section: Discussionmentioning

confidence: 74%

Superoxide-dependent consumption of nitric oxide in biological media may confound in vitro experiments

Keynes

Griffiths

Garthwaite

2003

Biochemical Journal

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NO functions ubiquitously as a biological messenger but has also been implicated in various pathologies, a role supported by many reports that exogenous or endogenous NO can kill cells in tissue culture. In the course of experiments aimed at examining the toxicity of exogenous NO towards cultured cells, we found that most of the NO delivered using a NONOate (diazeniumdiolate) donor was removed by reaction with the tissue-culture medium. Two NO-consuming ingredients were identified : Hepes buffer and, under laboratory lighting, the vitamin riboflavin. In each case, the loss of NO was reversed by the addition of superoxide dismutase. The effect of Hepes was observed over a range of NONOate concentrations (producing up to 1 µM NO). Furthermore, from measurements of soluble guanylate cyclase activity, Hepes-dependent NO consumption remained significant at the low nanomolar NO concentrations relevant to physiological NO

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Section: Discussionmentioning

confidence: 74%

Superoxide-dependent consumption of nitric oxide in biological media may confound in vitro experiments

Keynes

Griffiths

Garthwaite

2003

Biochemical Journal

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“…However, the Good's buffers including HEPES buffer have low affinities for transition-metal ions and are widely used in biological research. However, it was found that some of Good's buffers can give nitrogen-centered cation free radicals in the presence of Fe(II) [32] and can be oxidized by Cu(II) [33]. Recently, the research on the synthesis of gold nanoparticles by Good's buffers has been widely studied.…”

Section: Discussionmentioning

confidence: 99%

Fabrication of gold nanoparticles with different morphologies in HEPES buffer

Cheng

et al. 2010

Rare Metals

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Gold nanoparticles with different morphologies, such as spindle, octahedron, and decahedron were obtained by using different molar ratios of HAuCl 4 /HEPES in the presence and absence of surfactants at room temperature. These nanoparticles were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), scanning electron microcopy (SEM), energy-dispersive X-rays analysis (EDX), and selected area electron diffraction (SAED). The kinetics of the formation of gold nanoparticles in HEPES buffer was studied by UV-visible spectrophotometer. The formation of gold nanoparticles was strongly dependent on the concentration of HEPES and pH value. The surfactants play a crucial role in the size and shape controlled synthesis of gold nanoparticles.

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“…HEPES, PIPES, EPPS, bicine and MOPS interfere with Lowry protein determination; however, HEPES and MOPS do not interfere with Bradford or bicinchoninic acid assays. 4,96,105,106 3.1.5. Impact of buffers in redox studies.…”

Section: Hepesmentioning

confidence: 99%

(Un)suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions – a review

et al. 2015

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The use of buffers to maintain the pH within a desired range is a very common practice in chemical, biochemical and biological studies.Among them, zwitterionic N-substituted aminosulfonic acids, usually known as Good'sbuffers, although widely used, can complex metals and interact with biological systems. The present work reviews, discusses and updates the metal complexation characteristics of thirty one commercially available buffers. In addition, their impact on biological systems is also presented. The influences of these buffers on the results obtained in biological, biochemical and environmental studies, with special focus on their interaction with metal ions, are highlighted and critically reviewed. Using chemical speciation simulations, based on the current knowledge of the metal-buffer stability constants, a proposal of the most adequate buffer to employ for a given metal ion is presented.

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Radicals from “Good's” buffers

Cited by 128 publications

References 20 publications

Superoxide-dependent consumption of nitric oxide in biological media may confound in vitro experiments

Superoxide-dependent consumption of nitric oxide in biological media may confound in vitro experiments

Fabrication of gold nanoparticles with different morphologies in HEPES buffer

(Un)suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions – a review

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