This research investigated radio refractivity impact on signal strength of mobile communication. The mobile communication signal strengths of two popular networks in Nigeria, 9Mobile and MTN, were considered. In the 2100 MHz-3 G band, 9Mobile transmits in the downlink spectrum of 2130.00–2140.00 MHz, while MTN transmits in the downlink spectrum of 2110.00–2120.00 MHz. Also, 9Mobile transmits in the downlink spectrum of 791–821 MHz in the 800 MHz band and 1805–1880 MHz in the 1800 MHz, while MTN transmits in the downlink spectrums of 2620–2690 MHz in the 2600 MHz band; all in the 4 G band. Using the instrument of a mobile station in each station (location) in some selected cities in southern Nigeria, the signal strengths were measured. A cell signal monitor (version 5.1.1) mobile application installed in an Android (transceiver) device (having two SIM slots) constituted the mobile station. To achieve high accuracy, there was a restriction in measuring transmission from specific cells. Hourly measurement of signal strengths was carried out and instantaneously corresponding weather parameters were recorded. Weather parameters for this investigation; atmospheric temperature and pressure; and relative humidity were excerpted online from the Nigeria Meteorological Agency (NIMET) hourly weather report for the various cities where the stations were situated. The hourly radio refractivity was computed using the 2015 International Telecommunication Union–Radio-communication sector (ITU-R) recommended model. Overall, the results indicate that there was no established linear relationship between signal strength and radio refractivity since the overall average R value is 0.0123691 and the overall average standard deviation of R values is 0.1112165. The inconsistencies in the linear relationships obtained from different locations and cells could be due to variations in topography, antenna properties, seasonal variations, wind and position, and distance of the receiver from the transmitter.