Radioautographic visualization of radiocarbon in the organs and tissues of newborn rats following administration of C<sup>14</sup>‐labeled bicarbonate

Greulich, Richard C.; Leblond, C. P.

doi:10.1002/ar.1091150308

Cited by 50 publications

(11 citation statements)

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“…When carbon 14 was administered to newborn rats as labelled sodium bicarbonate, the isotope was observed first in the predentin (FIGURE 8) and later in the dentin 01 molars and incisors (R. C. Greulich and C. P. Leblond, 1953). I t was suggested in this work that the radioautographic reactions were not due FIGURES 4 a n d 5 .…”

Section: X360mentioning

confidence: 61%

“…More recently, the entry of radiocarbon into the matrix was systematically examined in a series of young rats killed a t intervals of from 1 to 15 days after the administration of (34-bicarbonate (C. P. Leblond et at., 1953; R. C. Creulich and C. P. Leblond, 1954). The C14 utilized in the synthesis of organic matrix by the dentinal organ appeared in radioautographic preparations in the form of a band which, with time, seemed to be farther and farther from the odontoblasts, but remained a t approximately the same distance from the dentino-enamel junction .…”

Section: Xi08mentioning

confidence: 99%

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Formation of Bones and Teeth as Visualized by Radioautography

Leblond

Bélanger

Greulich

1955

Annals of the New York Academy of Sciences

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Section: X360mentioning

confidence: 61%

Section: Xi08mentioning

confidence: 99%

Formation of Bones and Teeth as Visualized by Radioautography

Leblond

Bélanger

Greulich

1955

Annals of the New York Academy of Sciences

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“…Thus, lytic processes cause the resorption of cartilage along the metaphyseal surface of the plate (Schenk et al, 1967;Lee et al, 1995Lee et al, , 1998b, which results in its receding from the center of the bone. Meanwhile, synthetic activities lead to sufficient new cells and matrix to compensate for the surface loss (Walker and Kember, 1972;Belanger, 1954;Greulich and Leblond, 1953). The final result is that each plate is displaced farther and farther from the center of the bone, without significantly changing in thickness.…”

Section: Introductionmentioning

confidence: 99%

Active gelatinase B is identified by histozymography in the cartilage resorption sites of developing long bones

et al. 1999

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“…within the plate (Walker and Kember, 1972;Belanger, 1954;Greulich and Leblond, 1953). Therefore, during much of the growth period no significant changes take place in the plate size.…”

mentioning

confidence: 99%

Immunolocalization of the cleavage of the aggrecan core protein at the Asn341-Phe342 bond, as an indicator of the location of the metalloproteinases active in the lysis of the rat growth plate

et al. 1998

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In view of the extensive lysis of hyaline cartilage known to take place during endochondral bone formation, the current study was designed to test the hypothesis that metalloproteinases are the agents that mediate this lysis. Since these enzymes have been shown in vitro to cleave the core protein of the major proteoglycan of cartilage, aggrecan, at the Asn 341-Phe 342 bond, an immunohis-tochemical method has been developed to find out whether or not there are sites in the growth plate of the rat tibia where cleavage of this bond takes place. The cleavage of aggrecan by metalloproteinases is followed by the retention of the fragment known as G1, for it includes the G1 domain. Since the G1 fragment terminates in the amino acid residues ...FVDIPEN, we prepared an antiserum against FVDIPEN, confirmed its specificity, then applied it to the growth plate of 21-day-old rat tibia in the hope of localizing the G1 fragments. The antiserum specificity was shown by its recognition of the ...FVDIPEN sequence at the C-terminus of peptides and of G1 fragments produced by aggrecan cleavage. When the antiserum was applied to Western blots of guanidinium chloride extracts prepared from epiphyseal growth plate, it recognized two species (56 and 52 kDa), which differed only in the degree of glycosylation. These fragments were comparable in size to the G1 fragments generated by the action of recombinant metalloproteinase in vitro, thus confirming antiserum specificity for these fragments. Applying the antiserum to cryosections of 21-day-old rat tibiae revealed immunostaining at two intensities within the growth plate matrix: a strong staining was observed in a 1-5 µm-wide layer designated ''peripheral'' matrix, which borders the epiphyseal and metaphyseal marrow spaces as well as the perichondrium, while a weak staining was found in the rest of the plate, designated ''central'' matrix. The abundance of G1 fragments terminating in ...FVDIPEN in the peripheral matrix indicates that this is where the growth plate is lysed to achieve longitudinal and latitudinal bone growth. The site where metalloproteinases exert their main lytic activity is a thin layer of matrix separating central from peripheral matrix. Anat.

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Radioautographic visualization of radiocarbon in the organs and tissues of newborn rats following administration of C¹⁴‐labeled bicarbonate

Cited by 50 publications

References 19 publications

Formation of Bones and Teeth as Visualized by Radioautography

Formation of Bones and Teeth as Visualized by Radioautography

Active gelatinase B is identified by histozymography in the cartilage resorption sites of developing long bones

Immunolocalization of the cleavage of the aggrecan core protein at the Asn341-Phe342 bond, as an indicator of the location of the metalloproteinases active in the lysis of the rat growth plate

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Radioautographic visualization of radiocarbon in the organs and tissues of newborn rats following administration of C14‐labeled bicarbonate

Cited by 50 publications

References 19 publications

Formation of Bones and Teeth as Visualized by Radioautography

Formation of Bones and Teeth as Visualized by Radioautography

Active gelatinase B is identified by histozymography in the cartilage resorption sites of developing long bones

Immunolocalization of the cleavage of the aggrecan core protein at the Asn341-Phe342 bond, as an indicator of the location of the metalloproteinases active in the lysis of the rat growth plate

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Radioautographic visualization of radiocarbon in the organs and tissues of newborn rats following administration of C¹⁴‐labeled bicarbonate