Radiofrequency radiation at 2.856 GHz does not affect key cellular endpoints in neuron-like PC12 cells

Zhou, Hongmei; Dong, Guofu; Zheng, Wen; Wang, Shuiming; Wang, Lifeng; Zhi, Wei; Wang, Changzhen

doi:10.1080/15368378.2018.1550787

Cited by 2 publications

(1 citation statement)

References 56 publications

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“…Prenatal exposure to 2 W/kg 900 MHz RF-EMF for 1 h/day 21 days reduces the number of granule cells in the dentate gyrus in the rat hippocampus (Odaci et al, 2008). Other researchers found that RF-EMF exposure does not cause cell apoptosis (Joubert et al, 2007;Zhou et al, 2019). These inconsistent results suggested that much more studies are requested to get a robust conclusion, particularly to address much finer changes in cells except for cell death during brain development.…”

Section: Discussionmentioning

confidence: 99%

1800 MHz Radiofrequency Electromagnetic Field Impairs Neurite Outgrowth Through Inhibiting EPHA5 Signaling

Chen

Deng

et al. 2021

Front. Cell Dev. Biol.

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The increasing intensity of environmental radiofrequency electromagnetic fields (RF-EMF) has increased public concern about its health effects. Of particular concern are the influences of RF-EMF exposure on the development of the brain. The mechanisms of how RF-EMF acts on the developing brain are not fully understood. Here, based on high-throughput RNA sequencing techniques, we revealed that transcripts related to neurite development were significantly influenced by 1800 MHz RF-EMF exposure during neuronal differentiation. Exposure to RF-EMF remarkably decreased the total length of neurite and the number of branch points in neural stem cells-derived neurons and retinoic acid-induced Neuro-2A cells. The expression of Eph receptors 5 (EPHA5), which is required for neurite outgrowth, was inhibited remarkably after RF-EMF exposure. Enhancing EPHA5 signaling rescued the inhibitory effects of RF-EMF on neurite outgrowth. Besides, we identified that cAMP-response element-binding protein (CREB) and RhoA were critical downstream factors of EPHA5 signaling in mediating the inhibitory effects of RF-EMF on neurite outgrowth. Together, our finding revealed that RF-EMF exposure impaired neurite outgrowth through EPHA5 signaling. This finding explored the effects and key mechanisms of how RF-EMF exposure impaired neurite outgrowth and also provided a new clue to understanding the influences of RF-EMF on brain development.

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Section: Discussionmentioning

confidence: 99%

1800 MHz Radiofrequency Electromagnetic Field Impairs Neurite Outgrowth Through Inhibiting EPHA5 Signaling

Chen

Deng

et al. 2021

Front. Cell Dev. Biol.

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Radiofrequency Electromagnetic Field Exposure and Apoptosis: A Scoping Review of In Vitro Studies on Mammalian Cells

Romeo

Zeni

Scarfì

et al. 2022

IJMS

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In the last decades, experimental studies have been carried out to investigate the effects of radiofrequency (RF, 100 kHz–300 GHz) electromagnetic fields (EMF) exposure on the apoptotic process. As evidence-based critical evaluation of RF and apoptosis in vitro is lacking, we performed a scoping literature review with the aim of systematically mapping the research performed in this area and identifying gaps in knowledge. Eligible for inclusion were in vitro studies assessing apoptosis in mammalian cells exposed to RF-EMF, which met basic quality criteria (sham control, at least three independent experiments, appropriate dosimetry analysis and temperature monitoring). We conducted a systematic literature review and charted data in order to overview the main characteristics of included studies. From the 4362 papers retrieved with our search strategy, 121 were pertinent but, among them, only 42 met basic quality criteria. We pooled data with respect to exposure (frequency, exposure level and duration) and biological parameters (cell type, endpoint), and highlighted some qualitative trends with respect to the detection of significant effect of RF-EMF on the apoptotic process. We provided a qualitative picture of the evidence accumulated so far, and highlighted that the quality of experimental methodology still needs to be highly improved.

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Radiofrequency radiation at 2.856 GHz does not affect key cellular endpoints in neuron-like PC12 cells

Cited by 2 publications

References 56 publications

1800 MHz Radiofrequency Electromagnetic Field Impairs Neurite Outgrowth Through Inhibiting EPHA5 Signaling

1800 MHz Radiofrequency Electromagnetic Field Impairs Neurite Outgrowth Through Inhibiting EPHA5 Signaling

Radiofrequency Electromagnetic Field Exposure and Apoptosis: A Scoping Review of In Vitro Studies on Mammalian Cells

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