Radiographic Imaging as a Quality Index Proxy for Brachiaria brizantha Seeds

Abstract

Efficient methodologies for automated seed quality evaluations are important for the seed industry. Advanced seed technology research requires the use of adequate methods to ensure good seed performance under adverse environmental conditions; thus, providing producers with detailed, quick, and accurate information on structural seed integrity and ensuring vigorous production. To address this problem, this study aimed to determine Brachiaria brizantha (Marandu cv., Piatã cv. and Xaraés cv.) seed quality through radiographic imaging analyses associated with vigor tests and anatomical characterizations. Brachiaria seed cultivars displaying different physical and physiological attributes were selected and subjected to the 1000-seed weight test, water content determinations, X-ray analyses, germination tests, and anatomical characterizations. The X-ray analyses made it possible to establish a relationship between the X-ray images and other determined variables. Furthermore, the X-ray images can indicate evidence of internal and external damage that could later compromise germination. The Marandu and Piatã cultivars presented the highest germination percentages, germination speed indices, normal seedling development, and cellular structure preservation compared to the Xaraés cultivar. To summarize, X-ray analyses are efficient methods used for the selection of higher physical quality cultivars and can aid in the decision-making processes of companies and seed producers worldwide.

Applying YOLOv8 and X-ray Morphology Analysis to Assess the Vigor of Brachiaria brizantha cv. Xaraés Seeds

“…One effective strategy to enhance productivity involves the cultivation of high-vigor seeds [2][3][4]. The quality of these seeds, influenced by genetic, physical, sanitary, and physiological factors, profoundly impacts crop development, thereby directly affecting yield potential across plant species [5,6].…”

“…Moreover, these tests can be time-consuming, costly, and offer limited insights into the internal state of the seed [8]. Addressing this challenge, the assessment of internal seed morphology has emerged as a crucial approach for identifying issues related to seed physiological potential [6,9].…”

“…A well-established method for image acquisition relies on X-ray differential absorption by seed tissues, which varies with tissue thickness, density, composition, and radiation wavelength [15]. This process involves exposing seeds to X-rays, creating a latent image on a photosensitive film [6,11]. However, despite the assistance of computational tools, the manual measurement of parameters contributing to seed vigor remains a challenge in seed image analysis.…”