Radioimmunoassay for the Determination of the Steroidal Alkaloid Solasodine and Related Compounds in Living Plants and Herbarium Specimens¹

Abstract

A rapid, specific and sensitive radioimmunoassay for the determination of the steroidal alkaloid solasodine is described. The assay allows the detection of as little as 0.7 ng of solasodine glycosides (sola-' sonine, solarnargine) in crude plant extracts. The detailed distribution of solasodine in vegetative and generative parts of plants of Solanum laciniatum was investigated, and several thousand S. laciniatum plants were screened for individuals with high solasodine content. Plants which contained as much as 3.1 Oh of solasodine in the leaves were selected for further breeding. A taxonomic survey of the occurrence of solasodine in ca. 250 different species of the genus Solan-' Pan 13 in the series: "Use of lmmunoassay in Plant Sciences". For part 12 see: E. W. WEILER, Planta (in press).um using herbarium material is given. 0.5 mg of a herbarium sample is more than sufficient for quantitative analysis.

Solanum dulcamara L. (Bittersweet): Accumulation of Steroidal Alkaloids in the Plant and in Different in Vitro Systems

“…The direct identification of glycoalkaloids (e.g., solasonine, solamargine) is possible by HPLC (Elridge and Hockridge 1983;Hunter and Heftmann 1983; in combination with fast atom bombardment (FAB) or thermospray mass spectrometry (Price et al 1985;Ehmke et al 1987). For the sensitive and specific determination of solasodine glycosides in minute samples, Weiler et al (1980) established a radioimmunoassay. This can be a valuable method for the screening for solasodine-rich cell culture strains, and regenerated plants, but due to the high specificity, it is not possible to detect the accompanying sapogenins.…”

Development of novel IC-ELISA as a primary high throughput screening for various estrogen molecules

“…The CRs (%) between 17β-E2 and other steroidal compounds were determined using the following equation 28 : CR (%)=(concentration of 17β-E2 at B/B 0 =50%/ concentration of cross-reacting substance at B/B 0 = 50%) 100…”

Development of Eastern Blotting Technique for Analysis of Baicalin Using Anti-Baicalin Monoclonal Antibody

“…Immunoassay systems using monoclonal antibodies (MAbs) against bioactive compounds of low molecular weight have become important tools for the qualitative and quantitative analyses of active constituents from natural medicines and natural products [14]. The production and characterization of anti-BI MAb have already been described [15].…”