Radioimmunological Methods for the Estimation of Oestrone, Oestradiol 17β and Oestriol in Pregnancy Plasma

Lindberg, Bo; Lindberg, Paul; Martinsson, Κjell; Johansson, Elof D. B.

doi:10.3109/00016347409156389

Cited by 126 publications

(38 citation statements)

References 66 publications

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“…The radioimmunoassay for oestradiol was that described by Lindberg, Lindberg, Martinsson & Johansson (1974). The antiserum was raised in sheep against oestradiol-17-ß-oxime conjugated with BSA and used at a dilution of 1:100 000.…”

Section: Definition Of Hormonal Statementioning

confidence: 99%

Motor activity of the human Fallopian tube in vitro in relation to plasma concentration of oestradiol and progesterone, and the influence of noradrenaline

Helm¹,

Owman²,

Sjöberg³

et al. 1982

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The contractile pattern of the human Fallopian tube was studied in preparations from the ampullary and isthmic regions mounted in an organ bath for measurement of longitudinal and circular smooth muscle activity. The material was obtained during the follicular, ovulatory, and luteal phases of the cycle as defined primarily from plasma oestrogen and progesterone values. The frequency of the spontaneous contractions increased progressively during the follicular phase to become maximal around ovulation. There was no consistent difference between isthmus and ampulla; the circular musculature had a higher frequency than the longitudinal during the ovulatory phase. Noradrenaline (3 x 10(-6) M) in general potentiated the difference in frequency seen between the ovulatory phase on the one hand and the follicular and luteal phases on the other. Contractile activity, assessed by planimetric integration of the curve on the pen-recorder trace, increased markedly during the ovulatory phase in all types of smooth muscle preparations. Exogenous noradrenaline inhibited spontaneous motor activity in preparations from pregnant or post-menopausal women or from women taking combined-type oral contraceptives. This effect was most marked in the circular muscle. Thus the different regions of the human Fallopian tube in vitro show various patterns of spontaneous motor activity in relation to the plasma steroid concentrations furing the menstrual cycle. Responses to exogenous noradrenaline also varied, indicating that the effects of endogenous noradrenaline released from sympathetic nerves may vary similarly.

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Section: Definition Of Hormonal Statementioning

confidence: 99%

Motor activity of the human Fallopian tube in vitro in relation to plasma concentration of oestradiol and progesterone, and the influence of noradrenaline

Helm¹,

Owman²,

Sjöberg³

et al. 1982

Reproduction

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“…The specificity of the antisera used for the assays of progesterone [12] and oestradiol [13] has been described earlier. The dextran coated charcoal method was used for the separation of bound and unbound radioactivity.…”

Section: --mentioning

confidence: 99%

Metabolic control and serum hormone levels in relation to retinopathy in diabetic pregnancy

Laatikainen

Ranta

et al. 1982

Diabetologia

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Summary. The occurrence and progression of retinopathy were related to the mean blood glucose levels and the serum concentrations of prolactin, human placental lactogen, oestradiol and progesterone in 57 pregnant insulin-dependent diabetic patients. Fifteen patients had frank retinopathy, of whom eight showed a marked increase in retinopathy. The initial blood glucose levels were significantly higher in patients whose retinopathy progressed, whereas during the second and third trimester similar blood glucose levels were achieved in all groups. Serum concentrations of progesterone and human placental lactogen were significantly increased in diabetic patients during the last trimester when compared with those in normal pregnancies, and during the second trimester, patients with retinopathy showed significantly higher concentrations than those without, but no significant difference was found in oestradiol values. The eight patients with progressive retinopathy showed progesterone, human placental lactogen and oestradiol levels at or above the upper limit of the normal range. Throughout gestation, serum prolactin concentrations were significantly lower in diabetic patients than in healthy subjects. No correlation was found between serum prolactin values and the occurrence of retinopathy.Key words: Diabetes mellitus, diabetic pregnancy, diabetic retinopathy, metabolic control, human placental lactogen, progesterone, oestradiol, prolactin.Progression of diabetic retinopathy has been reported in 3%-29% of diabetic pregnancies [I, 3, 10, 11]. The main factors responsible for diabetic retinopathy are thought to be metabolic. During pregnancy, however, an increase in diabetic vasculopathy could also be related to the rapid and marked changes that occur with advancing gestation in the serum patterns of some steroid and protein hormones. In order to establish a possible correlation between progression of retinopathy, metabolic control and the hormonal status in pregnant diabetic patients, a prospective follow-up study comparing mean blood glucose levels and serum concentrations of prolactin, human placental lactogen, oestradiol and progesterone in patients with and without retinopathy, was performed. Patients and MethodsFifty-seven pregnant patients with Type 1 (insulin-dependent) diabetes treated at the I and II Departments of Obstetrics and Gynaecology of the Helsinki University Central Hospital in [1978][1979] were included in the study. In order to achieve good diabetic control, most patients were admitted to the obstetric ward for a short stay during the first trimester, usually between weeks 8 and 12 of pregnancy. All the patients were admitted to hospital during week 24, and from week 32 until delivery. Between these admissions, the patients were seen on an out-patient basis every second week and further admitted when needed.Ophthalmological examination, including direct and indirect ophthalmoscopy and colour photography of the fundus, was performed by one of the authors at the end of the first and second trimester, ...

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“…All samples were ana lyzed at the same time. For unconjugated estrone and estradiol, a sensitive RIA method earlier described was used [7,8]. The detection limit was 19 pmol/1 for Ei and Ei.…”

Section: Introductionmentioning

confidence: 99%

Endogenous Estrogen Levels in Postmenopausal Women with Severe Urogenital Atrophy

Nilsson¹,

Heimer

1992

Gynecol Obstet Invest

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In order to be able to evaluate the systemic influence of exogenous estrogen therapy in different conditions, it is important to have knowledge of the endogenous estrogen levels. The present study was undertaken to assess the basic levels and possible fluctuations of endogenous estrogen in postmenopausal women with urogenital estrogen deficiency. Plasma concentration levels of unconjugated estrone and estradiol were measured at regular intervals during 24 h in 5 postmenopausal women without estrogen treatment. All women had very low levels of estradiol. No diurnal fluctuation was found. Estrone levels were not measurable with a sensitive RIA technique.

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Radioimmunological Methods for the Estimation of Oestrone, Oestradiol 17β and Oestriol in Pregnancy Plasma

Cited by 126 publications

References 66 publications

Motor activity of the human Fallopian tube in vitro in relation to plasma concentration of oestradiol and progesterone, and the influence of noradrenaline

Motor activity of the human Fallopian tube in vitro in relation to plasma concentration of oestradiol and progesterone, and the influence of noradrenaline

Metabolic control and serum hormone levels in relation to retinopathy in diabetic pregnancy

Endogenous Estrogen Levels in Postmenopausal Women with Severe Urogenital Atrophy

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