Radiosensitivity and mutability of wheat seed progeny cultivated under adverse environments

Антонова, Е. В.; Khlestkina, Elena K.

doi:10.1016/j.plaphy.2019.02.011

Cited by 5 publications

(5 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar data on standard deviations have been presented by other researchers [22,80]. Additionally, an increase in the variation of root and shoot length has been revealed at the end of experiments in comparison with their beginning [18].…”

Section: Discussionsupporting

confidence: 87%

“…The laboratory seed germination method is widely used to assess the impact of individual environmental factors (e.g., low or high temperatures, salinity, moisture deficiency, ionizing radiation, heavy metals, nanoparticles, or magnetic fields) or their combined effects by means of biometric parameters (e.g., seed germination energy, seed vigor, seedling survival, and root and shoot lengths) at early stages of plant development [18][19][20][21][22][23][24][25][26]. In several studies, the roll culture method has been applied [19,20,25,27] or Petri dishes have been used [18,[21][22][23]28]. In these cases, experiments are conducted in distilled water, aqueous solutions, soil extracts, and suspensions with the adverse factors.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Germination and Growth Characteristics of nud Knockout and win1 Knockout Barley Lines under Salt Stress

Antonova,

Shimalina,

Korotkova

et al. 2024

Plants

Self Cite

View full text Add to dashboard Cite

Hordeum vulgare genes NUD (HvNUD) and WIN1 (HvWIN1) play a regulatory role in cuticle organization. Because the cuticle is a key evolutionary acquisition of plants for protection against environmental factors, a knockout (KO) of each gene may alter their ability to adapt to unfavorable conditions. A potential pleiotropic effect of HvNUD or HvWIN1 gene mutations can be assessed under salt stress. Initial developmental stages are the most sensitive in living organisms; therefore, we evaluated salt tolerance of nud KO and win1 KO barley lines at the seedling stage. Air-dried barley grains of the KO lines and of a wild-type (WT) line were germinated in NaCl solutions (50, 100, or 150 mM). Over 30 physiological and morphological parameters of seedlings were assessed. Potential pleiotropic effects of the HvNUD gene KO under salt stress included the stimulation of root growth (which was lower under control conditions) and root necrosis. The pleiotropic effects of the HvWIN1 gene KO under the stressful conditions manifested themselves as maintenance of longer root length as compared to the other lines; stable variation of most of morphological parameters; lack of correlation between root lengths before and after exposure to NaCl solutions, as well as between shoot lengths; and the appearance of twins. Salt tolerance of the analyzed barley lines could be ranked as follows: nud KO > win1 KO ≈ WT, where nud KO lines were the most salt-tolerant. A comparison of effects of salinity and ionizing radiation on nud KO and win1 KO barley lines indicated differences in tolerance of the lines to these stressors.

show abstract

Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Germination and Growth Characteristics of nud Knockout and win1 Knockout Barley Lines under Salt Stress

Antonova,

Shimalina,

Korotkova

et al. 2024

Plants

Self Cite

View full text Add to dashboard Cite

show abstract

“…In nud KO lines, mainly alterations of the shape of roots were registered here, such as hairy roots and the “dancer” phenotype. The latter anomaly has previously been described by us in seedlings of Triticum aestivum grown under adverse weather conditions [ 25 ]. According to our current findings, in the other KO lines ( win1 KO), anomalies of the color and shape of leaves are predominant.…”

Section: Discussionsupporting

confidence: 56%

“…The radiosensitivity of an organism depends on a number of parameters that characterize different types of variation: e.g., biochemical, genetic, ontogenetic, populational, species, ecological, geographical, spatial, and temporal [ 2 ]. Under conditions of chronic and acute irradiation, the radiosensitivity of plants can be modified by other environmental factors [ 25 , 26 ]. Altering the sensitivity of organisms to radiation is especially important now due to intensive development of the nuclear industry and climate change [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Seedling Biometry of nud Knockout and win1 Knockout Barley Lines under Ionizing Radiation

Антонова

Shimalina

Korotkova

et al. 2022

Plants

Self Cite

View full text Add to dashboard Cite

The genes NUD and WIN1 play a regulatory role in cuticle organization in barley. A knockout (KO) of each gene may alter plant mechanisms of adaptation to adverse environmental conditions. A putative pleiotropic effect of NUD or WIN1 gene mutations in barley can be assessed in a series of experiments in the presence or absence of a provoking factor. Ionizing radiation is widely used in research as a provoking factor for quantifying adaptive potential of living organisms. Our aim was to evaluate initial stages of growth and development of barley lines with a KO of NUD or WIN1 under radiation stress. Air-dried barley grains with different KOs and wild-type control (WT) were exposed to γ-radiation at 50, 100, or 200 Gy at a dose rate of 0.74 R/min. Approximately 30 physiological parameters were evaluated, combined into groups: (1) viability, (2) radiosensitivity, and (3) mutability of barley seed progeny. Seed germination, seedling survival, and shoot length were similar among all barley lines. Naked nud KO lines showed lower weights of seeds, roots, and seedlings and shorter root length as compared to win1 KO lines. The shoot-to-root length ratio of nud KO lines’ seedlings exceeded that of win1 KO and WT lines. In terms of the number of seedlings with leaves, all the KO lines were more sensitive to pre-sowing γ-irradiation. Meanwhile, the radioresistance of nud KO lines (50% growth reduction dose [RD50] = 318–356 Gy) and WT plants (RD50 = 414 Gy) judging by seedling weight was higher than that of win1 KO lines (RD50 = 201–300 Gy). Resistance of nud KO lines to radiation was also demonstrated by means of root length (RD50 = 202–254 Gy) and the shoot-to-root length ratio. WT seedlings had the fewest morphological anomalies. In nud KO lines, mainly alterations of root shape were found, whereas in win1 KO lines, changes in the color and shape of leaves were noted. Thus, seedlings of nud KO lines are characterized mainly by changes in the root system (root length, root number, and root anomalies). For win1 KO lines, other parameters are sensitive (shoot length and alterations of leaf shape and color). These data may indicate a pleiotropic effect of genes NUD and WIN1 in barley.

show abstract

“…After ion irradiation in rice, the growth retardation and death of rice are related to early dose and time-related gene expression changes [24]. In wheat, gamma irradiation can stimulate increase in the number of wheat seedlings with various developmental disorders and wheat seed progeny also had a lower seedling survival rate and weight [25]. In other plants, it has been demonstrated that plants alter the contents of a range of primary metabolites, such as carbohydrates, organic acids, and amino acids in response to UV-B radiation [26,27,28].…”

Section: Discussionmentioning

confidence: 99%

Global Transcriptome and Co-Expression Network Analysis Reveal Contrasting Response of Japonica and Indica Rice Cultivar to γ Radiation

Zhang

Huang

et al. 2019

IJMS

View full text Add to dashboard Cite

Japonica and indica are two important subspecies in cultivated Asian rice. Irradiation is a classical approach to induce mutations and create novel germplasm. However, little is known about the differential response between japonica and indica rice after γ radiation. Here, we utilized the RNA sequencing and Weighted Gene Co-expression Network Analysis (WGCNA) to compare the transcriptome differences between japonica Nipponbare (NPB) and indica Yangdao6 (YD6) in response to irradiation. Japonica subspecies are more sensitive to irradiation than the indica subspecies. Indica showed a higher seedling survival rate than japonica. Irradiation caused more extensive DNA damage in shoots than in roots, and the severity was higher in NPB than in YD6. GO and KEGG pathway analyses indicate that the core genes related to DNA repair and replication and cell proliferation are similarly regulated between the varieties, however the universal stress responsive genes show contrasting differential response patterns in japonica and indica. WGCNA identifies 37 co-expressing gene modules and ten candidate hub genes for each module. This provides novel evidence indicating that certain peripheral pathways may dominate the molecular networks in irradiation survival and suggests more potential target genes in breeding for universal stress tolerance in rice.

show abstract

Radiosensitivity and mutability of wheat seed progeny cultivated under adverse environments

Cited by 5 publications

References 23 publications

Germination and Growth Characteristics of nud Knockout and win1 Knockout Barley Lines under Salt Stress

Germination and Growth Characteristics of nud Knockout and win1 Knockout Barley Lines under Salt Stress

Seedling Biometry of nud Knockout and win1 Knockout Barley Lines under Ionizing Radiation

Global Transcriptome and Co-Expression Network Analysis Reveal Contrasting Response of Japonica and Indica Rice Cultivar to γ Radiation

Contact Info

Product

Resources

About