Radiosensitivity of CD3−CD8+CD56+ NK cells

Vokurková, Doris; Vávrová, Jiřina; Sinkora, J; Stoklasová, A; Blaha, V.; Řezáčová, Martina

doi:10.1016/j.radmeas.2010.07.028

Cited by 11 publications

(6 citation statements)

References 11 publications

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“…CD8+ NK cells (CD8+ [%] in CD56+CD3-) are highly functional; they exhibit a more functional profile (as detected by cytokine production and degranulation) and are inversely associated with HIV-related disease markers and linked with delayed disease progression [28]; they are also more radio-resistant [29] and more cytotoxic which facilitates tumor killing [30].…”

Section: Discussionmentioning

confidence: 99%

Comparison of T and NK lymphocyte subsets between human endometrial tissue and peripheral blood

Chernyshov

Dons’koi

Sudoma

et al. 2019

cejoi

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Immune profiles in endometrium may be changed in patients with IVF failure and its possible correlations with immune parameters in peripheral blood are important for the diagnostic approach. Such correlations in healthy women are unknown and have been studied in the present research. The expression of CD56, CD158a, HLA DR, CD69 in T lymphocytes, CD4 T lymphocytes, CD8+ T lymphocytes and NK cells were studied by flow-cytometry in endometrium and peripheral blood in healthy 24 donors of oocytes aged 25-32 years. Levels of T lymphocyte and T helper cells were lower in endometrium and no differences in CD8 T lymphocytes were registered between endometrium and peripheral blood. The expression of HLA DR and especially CD69 was higher in CD3, CD4, CD8 T cells in endometrium in comparison with peripheral blood. The endometrium lymphocyte population was enriched by NK cells that were generally CD56++ with a higher expression of HLA DR and almost in total were CD69 positive. Strong positive correlations of CD8 expression in NK cells (r = 0.6478, p < 0.001) and HLA DR expression in CD8 T cells (r = 0.6107, p < 0.01) between peripheral blood and endometrium were registered in fertile women. The endometrial CD56 expression in CD8+ T cells negatively correlated with endometrial CD8 expression in NK cells (r = –0.5252, p < 0.01) which possibly reflected a suppressive and regulating mechanism in the endometrium. CD8+ NK cells and HLA DR+ CD8 T cells in endometrium were related to the same subsets in peripheral blood.

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Section: Discussionmentioning

confidence: 99%

Comparison of T and NK lymphocyte subsets between human endometrial tissue and peripheral blood

Chernyshov

Dons’koi

Sudoma

et al. 2019

cejoi

View full text Add to dashboard Cite

show abstract

“…Reports on radiation sensitivity of NK cells have been contradictory (22–26), and our ex vivo culture conditions might affect radiation sensitivity, so we used flow cytometry to measure the numbers of different blood cell subtypes (T-cells, B-cells and NK cells) in our ex vivo culture model at 48 h after exposure to 0, 2, 5 or 8 Gy γ rays. The samples exposed to 8 Gy did not yield sufficient cell counts in the flow cytometry assay to provide reliable data.…”

Section: Resultsmentioning

confidence: 99%

Widespread Decreased Expression of Immune Function Genes in Human Peripheral Blood Following Radiation Exposure

2013

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“…But for tumor tissue, the situation is much more complicated. The CD3 + CD8 + cell populations in tumors represent the infiltration of cytotoxic T cells in the tumor sites, while the CD3 − CD8 + cell populations can represent NK cells in the tumor sites in this study [ 23 ]. Therefore, we stained the cells derived from the spleen and tumor in the Lewis lung carcinoma-bearing mouse model using an anti-CD3 antibody to clarify this difference in splenocytes and tumor cells.…”

Section: Discussionmentioning

confidence: 99%

Synergistic Effects of Metal–Organic Nanoplatform and Guanine Quadruplex-Based CpG Oligodeoxynucleotides in Therapeutic Cancer Vaccines with Different Tumor Antigens

Li,

Ebara,

Shirahata

et al. 2024

Vaccines

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Oligodeoxynucleotides (ODNs) containing unmethylated cytosine–phosphate–guanosine (CpG) motifs are readily recognized by Toll-like receptor 9 on immune cells, trigger an immunomodulatory cascade, induce a Th1 -biased immune milieu, and have great potential as an adjuvant in cancer vaccines. In this study, a green one-step synthesis process was adopted to prepare an amino-rich metal–organic nanoplatform (FN). The synthesized FN nanoplatform can simultaneously and effectively load model tumor antigens (OVA)/autologous tumor antigens (dLLC) and immunostimulatory CpG ODNs with an unmodified PD backbone and a guanine quadruplex structure to obtain various cancer vaccines. The FN nanoplatform and immunostimulatory CpG ODNs generate synergistic effects to enhance the immunogenicity of different antigens and inhibit the growth of established and distant tumors in both the murine E.G7-OVA lymphoma model and the murine Lewis lung carcinoma model. In the E.G7-OVA lymphoma model, vaccination efficiently increases the CD4+, CD8+, and tetramer+CD8+ T cell populations in the spleens. In the Lewis lung carcinoma model, vaccination efficiently increases the CD3+CD4+ and CD3+CD8+ T cell populations in the spleens and CD3+CD8+, CD3−CD8+, and CD11b+CD80+ cell populations in the tumors, suggesting the alteration of tumor microenvironments from cold to hot tumors.

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Radiosensitivity of CD3−CD8+CD56+ NK cells

Cited by 11 publications

References 11 publications

Comparison of T and NK lymphocyte subsets between human endometrial tissue and peripheral blood

Comparison of T and NK lymphocyte subsets between human endometrial tissue and peripheral blood

Widespread Decreased Expression of Immune Function Genes in Human Peripheral Blood Following Radiation Exposure

Synergistic Effects of Metal–Organic Nanoplatform and Guanine Quadruplex-Based CpG Oligodeoxynucleotides in Therapeutic Cancer Vaccines with Different Tumor Antigens

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