Radiotherapy in combination with hyperthermia suppresses lung cancer progression via increased NR4A3 and KLF11 expression

Son, Bong Soo; Jeon, Jaewan; Lee, Sung-Min; Kim, Hyun Woo; Kang, Hyunkoo; Youn, HyeSook; Jo, Sunmi; Youn, BuHyun

doi:10.1080/09553002.2019.1665213

Cited by 21 publications

(17 citation statements)

References 48 publications

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“…In addition, KLF expression patterns can change dramatically during development, such that some members may overlap at some developmental stages but not at others. Although KLF11 expression is decreased in colon, kidney, lung, ovary, and pancreas cancers, 11 it is increased in gastric and breast cancers. 3 , 6 In the present study, we collected data from 116 glioma patients to detect KLF11 expression by IHC assay.…”

Section: Discussionmentioning

confidence: 97%

KLF11 Expression Predicts Poor Prognosis in Glioma Patients

Xi¹,

Zhang²,

Zhang³

et al. 2021

IJGM

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Purpose Glioma is a primary intracranial malignant tumor with high recurrence and mortality rates. It is very important to study the prognostic factors. KLF11 can function as an oncogene or a tumor suppressor, depending on the tumor and tissue types and the cancer stage. In this study, we aimed to determine whether KLF11 expression is related to the overall survival of glioma patients. Patients and Methods We investigated KLF11 expression in 116 glioma patients with different grades using Western blot and immunohistochemistry assay. We analyzed the patients with different glioma grades and KLF11 expression levels by Kaplan–Meier survival curves. Independent prognostic factors for poor overall survival were identified by univariate and multivariate analyses. Results There were 37 patients in KLF11 low expression group and 79 patients in high expression group. There was no difference in gender, age, tumor diameter or tumor location between two groups. The patients in KLF11 high expression group had higher ECOG score ( P =0.025) and higher WHO grades ( P =0.029). Western blot and immunohistochemistry assay showed KLF11 expression was significantly upregulated in glioma groups compared with normal brain tissues group ( P < 0.05), and the expression in grades III–IV was significantly higher than those in grades I–II ( P < 0.05). Kaplan–Meier survival curve analysis showed high KLF11 expression tended to reduce the overall survival ( P < 0.05). After univariate and multivariate analyses, KLF11 expression ( P =0.003) and age ( P =0.007) were independent prognostic factors for poor survival in glioma patients. Conclusion KLF11 expression was increased in glioma tissues, and high KLF11 expression was associated with poor prognosis. KLF11 expression was an independent prognostic factor for poor survival in glioma patients. KLF11 may serve as a novel prognostic marker for gliomas and as a novel treatment target.

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Section: Discussionmentioning

confidence: 97%

KLF11 Expression Predicts Poor Prognosis in Glioma Patients

Xi¹,

Zhang²,

Zhang³

et al. 2021

IJGM

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“…As previously described, the human colorectal cancer cell line Caco-2 and the human normal hepatocyte cell line MIHA were cultured [ 24 ]. Briefly, MEM (for Caco-2) and DMEM (for MIHA) were utilized supplemented with 10% FBS and penicillin (100 U/mL), and streptomycin (100 mg/mL), respectively.…”

Section: Methodsmentioning

confidence: 99%

Soybean-Derived Peptides Attenuate Hyperlipidemia by Regulating Trans-Intestinal Cholesterol Excretion and Bile Acid Synthesis

et al. 2021

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Increased triglyceride, cholesterol, and low-density lipoprotein (LDL) levels cause hyperlipidemia. Despite the availability of statin-based drugs to reduce LDL levels, additional effective treatments for reducing blood lipid concentrations are required. Herein, soybean hydrolysate prepared via peptic and tryptic hydrolysis promoted trans-intestinal cholesterol excretion (TICE) by increasing ATP-binding cassette subfamily G member 5 (ABCG5) and ABCG8 expression. The peptide sequence capable of promoting TICE was determined via HPLC and LC-MS/MS. Based on this, pure artificial peptides were synthesized, and the efficacy of the selected peptides was verified using cellular and hyperlipidemic mouse models. Soybean hydrolysates, including two bioactive peptides (ALEPDHRVESEGGL and SLVNNDDRDSYRLQSGDAL), promoted TICE via the expression of ABCG5 and ABCG8 in enterocytes. They downregulated expression of hepatic cytochrome P450 family 7 subfamily A member 1 (CYP7A1) and CYP8B1 via expression of fibroblast growth factor 19 (FGF19) in a liver X receptor α (LXRa)-dependent pathway. Administration of bioactive peptides to hyperlipidemic mouse models by oral gavage reduced cholesterol levels in serum via upregulation of ABCG5 and ABCG8 expression in the proximal intestine and through fecal cholesterol excretion, upregulated FGF 15/19 expression, and suppressed hepatic bile acid synthesis. Oral administration of soybean-derived bioactive peptides elicited hypolipidemic effects by increasing TICE and decreasing hepatic cholesterol synthesis.

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“…Human colorectal cancer cell line Caco-2 and human normal hepatocyte cell line MIHA were cultured following the previous study [ 21 ]. In short, the culture medium was prepared based on MEM (for Caco-2) and DMEM (for MIHA) supplemented with 10% of FBS and 1% of penicillin and streptomycin.…”

Section: Methodsmentioning

confidence: 99%

Hypolipidemic Roles of Casein-Derived Peptides by Regulation of Trans-Intestinal Cholesterol Excretion and Bile Acid Synthesis

Lee

Youn

2020

Nutrients

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Hyperlipidemia, a syndrome characterized by an abnormal elevation of blood lipids, causes chronic lethal metabolic disorders. Although statins are regularly prescribed to patients, an alternative to treat the burden of excessive lipids is required for cholesterol control. In this study, it was found that the treatment of casein hydrolyzed by pepsin and trypsin induced trans-intestinal cholesterol excretion (TICE) through ATP-binding cassette subfamily G members 5 (ABCG5) expression. Next, we analyzed sequences of the peptides responsible for TICE induction, synthesized artificial peptides based on the sequences, and the hypolipidemic effects of the peptide treatments were assessed in both in vitro and in vivo models. We determined that two bioactive peptides contained in casein hydrolysates (SQSKVLPVPQK and HPHPHLSF) induced TICE through the expression of ABCG5 in enterocytes and suppressed hepatic mRNA expression of cytochrome P450 family 7 subfamily A member 1 (CYP7A1) and CYP8B1by ileal FGF19 expression both in an liver X receptor α (LXRα)-mediated manner. In the hyperlipidemic mouse models, the oral administration of peptides reduced serum cholesterol levels through elevation of the ABCG5 expression in proximal intestine and fecal cholesterol secretion. Besides this, peptides induced ileal expression of fibroblast growth factor 15/19 (FGF15/19) and inhibited hepatic bile acid synthesis. We found that the oral treatment of casein-derived bioactive peptides could improve hyperlipidemia by regulating intestinal excretion and hepatic synthesis of cholesterols.

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Radiotherapy in combination with hyperthermia suppresses lung cancer progression via increased NR4A3 and KLF11 expression

Cited by 21 publications

References 48 publications

KLF11 Expression Predicts Poor Prognosis in Glioma Patients

KLF11 Expression Predicts Poor Prognosis in Glioma Patients

Soybean-Derived Peptides Attenuate Hyperlipidemia by Regulating Trans-Intestinal Cholesterol Excretion and Bile Acid Synthesis

Hypolipidemic Roles of Casein-Derived Peptides by Regulation of Trans-Intestinal Cholesterol Excretion and Bile Acid Synthesis

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