Ram seminal plasma proteome and its impact on liquid preservation of spermatozoa

Soleilhavoup, Clément; Tsikis, Guillaume; Labas, Valérie; Harichaux, Grégoire; Kohnke, Philippa; Dacheux, Jean‐Louis; Guérin, Y.; Gatti, Jean‐Luc; Graaf, S.P. de; Druart, Xavier

doi:10.1016/j.jprot.2014.07.007

Cited by 80 publications

(78 citation statements)

References 62 publications

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“…In the sheep, characterisation of such protein specific variation between rams has recently been undertaken based on liquid semen preservation ability. A number of proteins were found to be more or less abundant in high and low preservation ability groups [24]. Similar correlations between identified protein abundance and ram sperm freezing ability have not been performed.…”

Section: Introductionmentioning

confidence: 90%

The identification of proteomic markers of sperm freezing resilience in ram seminal plasma

Rickard

Leahy

Soleilhavoup

et al. 2015

Journal of Proteomics

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Section: Introductionmentioning

confidence: 90%

The identification of proteomic markers of sperm freezing resilience in ram seminal plasma

Rickard

Leahy

Soleilhavoup

et al. 2015

Journal of Proteomics

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“…The production of ROS can negatively affect the resilience of spermatozoa to normal metabolic changes that influence plasma membrane and DNA integrity (Aurich, 2005). The dilution of the sample during the SUT could also result in a dilution of protective proteins in the seminal plasma, which would inhibit the spermatozoa's ability to be resilient to the changes induced by ROS that are produced during normal sperm metabolism (Soleilhavoup et al, 2014). The use of the SUT, although it favours the selection of the most motile spermatozoa in a sample, may result in spermatozoa being discriminated against in the female reproductive tract.…”

Section: Resultsmentioning

confidence: 99%

A novel flush technique to simulate natural dispersal of spermatozoa in the female reproductive tract and expedite motility assessment of fresh ejaculated Merino (<i>Ovis aries</i>) sperm

Boshoff¹,

Lambrechts²,

Maree³

et al. 2018

SA J. An. Sci.

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Motility is an important criterion in the assessment and quantification of the quality of ejaculated and epididymal sperm samples. Ovine sperm spermatozoa are particularly susceptible to cryodamage, and shortening the interval from collection to cryostorage may potentially minimize the negative effects of cryopreservation, thereby improving the post-thaw viability of ram spermatozoa. The use of the swim-up technique (SUT) in quantifying spermatozoa motility is well documented, especially for the isolation of highly motile spermatozoa for assisted reproductive purposes. However, this technique is time consuming and involves a swim-up period of 10 minutes before the motility of a sample is recorded by using computerassisted sperm analysis (CASA) software such as the Sperm Class Analyser® (SCA®). The novel flush technique (FT) allows for capturing of sperm motility tracks via the SCA® system shortly after semen collection, that is, within a minute. This study compared fresh ejaculated sperm motility traits by using the SUT and FT. Motility evaluations were performed using 45 semen samples obtained from 15 adult Merino rams (Ovis aries) at weekly intervals. Motility recordings were captured at 100 frames per second, using a calibrated 20 µm deep Leja slide. The percentage total motile spermatozoa of samples subjected to the FT was 89.2%, which was significantly higher than that recorded by the SUT (83.9%). The results also indicated that the wobble (WOB) parameter showed significantly higher values when using the FT, and parameters curvilinear velocity (VCL) and amplitude of the lateral head displacement (ALH) indicated significantly higher values when using the SUT. Establishing the ideal spermatozoa concentration and analysis of sperm subpopulation motility characteristics would assist in the optimization of the FT, and its use in CASA motility analysis of ovine spermatozoa. Standardization of CASA analysis of ovine semen samples, which would enable the selection of quality spermatozoa samples for use in field insemination (fresh samples) or in vitro fertilization programs, and laparoscopic AI cryopreserved samples warrants further investigation.

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“…Among those, 14 correlated negatively (Pearson correlation coefficients from -0.85 to -0.43; p<0.05) and 4 correlated positively (0.52 to 0.44) with fertility. From this set of 18 proteins, 4 had previously been found to be related to sperm quality, motility and/or freezing ability in sheep (Rickards et al, 2014, 2015, Soleilhavoup et al, 2014. An interesting example is Ubiquitin carboxyl-terminal hydrolase isozyme L3, which increases in abundance with fertility.…”

Section: Methodsmentioning

confidence: 99%

“…Differences in fertility among bulls with apparently similar sperm quality have been related to variations in SP content and its effects on spermatozoa (Maxwell et al, 2007). Sets of proteins from seminal plasma have been associated with sperm motility, resilience to freezing and survival in the genital tract in the sheep (Rickard et al, 2014(Rickard et al, , 2015Soleilhavoup et al, 2014). In addition, SP from bulls of low fertility negatively affected the viability of bovine endometrial epithelial cells in culture .…”

Section: Id Usmentioning

confidence: 99%

Submitted oral and poster presentations

2018

Advances in Animal Biosciences

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Ram seminal plasma proteome and its impact on liquid preservation of spermatozoa

Cited by 80 publications

References 62 publications

The identification of proteomic markers of sperm freezing resilience in ram seminal plasma

The identification of proteomic markers of sperm freezing resilience in ram seminal plasma

A novel flush technique to simulate natural dispersal of spermatozoa in the female reproductive tract and expedite motility assessment of fresh ejaculated Merino (<i>Ovis aries</i>) sperm

Submitted oral and poster presentations

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