Raman fiber laser for the drug-free photodynamic therapy

Yusupov, A. S.; Goncharov, S. E.; Zalevskii, I D; Paramonov, V. M.; Kurkov, Andrei S

doi:10.1134/s1054660x10040134

Cited by 50 publications

(34 citation statements)

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“…To induce cell death, we irradiate cells with a fluence that is three orders of magnitude greater than the one used in Ref. (37). Such a difference does not imply that cytotoxic effects reported in vivo are not due to 1 O 2 creation (37).…”

Section: Resultsmentioning

confidence: 99%

Cancerous Cell Death from Sensitizer Free Photoactivation of Singlet Oxygen

Anquez

Yazidi‐Belkoura

Randoux

et al. 2011

Photochem & Photobiology

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Singlet oxygen ((1)O(2)) is an electronic state of molecular oxygen which plays a major role in many chemical and biological photo-oxidation processes. It has a high chemical reactivity which is commonly harnessed for therapeutic issues. Indeed, (1)O(2) is believed to be the major cytotoxic agent in photodynamic therapy. In this treatment of cancer, (1)O(2) is created, among other reactive species, by an indirect transfer of energy from light to molecular oxygen via excitation of a photosensitizer (PS). This PS is believed to be necessary to obtain an efficient (1)O(2) production. In this paper, we demonstrate that production of (1)O(2) is achieved in living cells from PS-free 1270 nm laser excitation of molecular oxygen. The quantity of (1)O(2) produced in this way is sufficient to induce an oxidative stress leading to cell death. Other effects such as thermal stress are discriminated and we conclude that cell death is only due to (1)O(2) creation. This new simplified scheme of (1)O(2) activation can be seen as a breakthrough for phototherapies of malignant diseases and/or as a noninvasive possibility to generate reactive oxygen species in a tightly controlled manner.

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Section: Resultsmentioning

confidence: 99%

Cancerous Cell Death from Sensitizer Free Photoactivation of Singlet Oxygen

Anquez

Yazidi‐Belkoura

Randoux

et al. 2011

Photochem & Photobiology

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“…Several experimental aspects are detailed in each entry (see footnotes for more information). Each set of conditions has been obtained from a publication, with the particular reference provided in each case. Transition Biological model Light source Wavelength Power Spectral control 1 O 2 scavenging 1 O 2 enhancement Thermal control Reference 1270 nm Human erythrocytesDiode laser (cw)1264 nm0.5–2 mWYespN 2 NoNo[108]T36 carcinoma in miceDiode laser (cw)1264 nm4–4.5 mWNoNoNoNo[108]Human skin tumorsRaman fiber laser (cw)1262 nm1.5–5 WNoNoNoNo[23]MCF-7 breast cancer lineRaman fiber laser (cw)1270 nm1.5–5 WYes-BSApO 2 Yes[24]-NaN 3 -pN 2 CC-5 carcinoma in miceRaman fiber laser (cw)1265 nm4 WNoNoNoNo[26]-HaCaTDiode laser (cw)1268 nm500 mWYesα-TOCNoYes[27]-Primary Keratinocytes-HeLaMCF-7 breast cancer lineRaman fiber laser (cw)1270 nm300 mWYes-BSApO 2 Yes[116]-NaN 3 -pN 2 -HaCaTDiode laser (cw)1268 nm–NoNoNoNo[119]-Primary Keratinocytes-HeLa-HCT-116 colorectal lineRaman fiber laser (cw)1265 nm4 WNo…”

Section: Direct Optical Excitation Of 1o2mentioning

confidence: 99%

Direct 1O2 optical excitation: A tool for redox biology

Blázquez‐Castro

2017

Redox Biology

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Molecular oxygen (O2) displays very interesting properties. Its first excited state, commonly known as singlet oxygen (1O2), is one of the so-called Reactive Oxygen Species (ROS). It has been implicated in many redox processes in biological systems. For many decades its role has been that of a deleterious chemical species, although very positive clinical applications in the Photodynamic Therapy of cancer (PDT) have been reported. More recently, many ROS, and also 1O2, are in the spotlight because of their role in physiological signaling, like cell proliferation or tissue regeneration. However, there are methodological shortcomings to properly assess the role of 1O2 in redox biology with classical generation procedures. In this review the direct optical excitation of O2 to produce 1O2 will be introduced, in order to present its main advantages and drawbacks for biological studies. This photonic approach can provide with many interesting possibilities to understand and put to use ROS in redox signaling and in the biomedical field.

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“…Although biological action of IR lasers was clearly demonstrated [6][7][8][9][10][11] there was no crucial evidence showing that laser damage of tumor cells was due namely to oxygen excitation. In this connection, a decade ago our group started work on mimicking these effects in solutions of singlet ( 1 Δ g ) oxygen traps in organic solvents and water at normal pressure and temperature.…”

Section: Introductionmentioning

confidence: 99%