Raman imaging as a new analytical tool for the quality control of the monitoring of osteogenic differentiation in forming 3D bone tissue

Bleye, Charlotte De; Fontaine, M; Dumont, Elodie; Sacré, Pierre-Yves; Hubert, Ph.; Theys, Nicolas; Ziemons, Eric

doi:10.1016/j.jpba.2020.113319

Cited by 8 publications

(10 citation statements)

References 32 publications

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“…The use of confocal Raman for biological applications has recently been reviewed by Gomes da Costa et al 43 CRM is used in many different fields such as drug delivery, 44 diagnosis of diseases such as cancer 45 or tissue engineering. 46 In histopathology, Raman imaging, also called hyperspectral Raman imaging, is a valuable complementary tool to detect, locate and delineate diseased tissues. Raman imaging has shown to improve diagnostic accuracy as it reveals a deep molecular understanding of the biochemical changes in affected tissues in comparison with healthy tissue.…”

Section: Raman Scatteringmentioning

confidence: 99%

“…Figure details the workflow for tissue analyses by confocal Raman spectroscopy. The use of confocal Raman for biological applications has recently been reviewed by Gomes da Costa et al CRM is used in many different fields such as drug delivery, diagnosis of diseases such as cancer, or tissue engineering . In histopathology, Raman imaging, also called hyperspectral Raman imaging, is a valuable complementary tool to detect, locate, and delineate diseased tissues.…”

Section: Vibrational Spectroscopies and Imagingmentioning

confidence: 99%

“…The simplest approach for exploring data is a univariate peak analysis by imaging the intensities of a single Raman peak. ,, However, the univariate methodology may, in some cases, lead to limited molecular specificity in complex tissues due to the overlapping of component signatures. To override this limitation, some effort has been made to develop probes for FTIR imaging through the same pathways as used in fluorescence imaging, for example .…”

Section: Vibrational Spectroscopies and Imagingmentioning

confidence: 99%

“…recently been reviewed by Gomes da Costa et al 43 CRM is used in many different fields such as drug delivery, 44 diagnosis of diseases such as cancer, 45 or tissue engineering. 46 45 To enhance spectral quality, Raman imaging may require long acquisition times. Many efforts were deployed by researchers to improve data acquisition rate for spontaneous Raman tools such as CRM.…”

Section: ■ Vibrational Spectroscopies and Imagingmentioning

confidence: 99%

See 3 more Smart Citations

Multimodal Imaging Based on Vibrational Spectroscopies and Mass Spectrometry Imaging Applied to Biological Tissue: A Multiscale and Multiomics Review

et al. 2020

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Section: Raman Scatteringmentioning

confidence: 99%

Section: Vibrational Spectroscopies and Imagingmentioning

confidence: 99%

Section: Vibrational Spectroscopies and Imagingmentioning

confidence: 99%

Section: ■ Vibrational Spectroscopies and Imagingmentioning

confidence: 99%

See 2 more Smart Citations

Multimodal Imaging Based on Vibrational Spectroscopies and Mass Spectrometry Imaging Applied to Biological Tissue: A Multiscale and Multiomics Review

et al. 2020

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“…They found that the characteristic signatures of several specific macromolecules of the extracellular matrix (ECM) were identifiable in the early stages of condensation, including collagen type II, proteoglycans, adhesion molecules, and several other proteins, whereas, in the latter stages, the elaboration of the lipidic content of the ECM appeared to be the most significant. Using Raman imaging, De Bleye et al [ 174 ] monitored the synthesis of ECM and its progressive mineralization during the osteogenic differentiation process.…”

Section: Latest Technological Advances In Monitoring Stem Cell Status...mentioning

confidence: 99%

Recent Advances in Monitoring Stem Cell Status and Differentiation Using Nano-Biosensing Technologies

et al. 2022

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In regenerative medicine, cell therapies using various stem cells have received attention as an alternative to overcome the limitations of existing therapeutic methods. Clinical applications of stem cells require the identification of characteristics at the single-cell level and continuous monitoring during expansion and differentiation. In this review, we recapitulate the application of various stem cells used in regenerative medicine and the latest technological advances in monitoring the differentiation process of stem cells. Single-cell RNA sequencing capable of profiling the expression of many genes at the single-cell level provides a new opportunity to analyze stem cell heterogeneity and to specify molecular markers related to the branching of differentiation lineages. However, this method is destructive and distorted. In addition, the differentiation process of a particular cell cannot be continuously tracked. Therefore, several spectroscopic methods have been developed to overcome these limitations. In particular, the application of Raman spectroscopy to measure the intrinsic vibration spectrum of molecules has been proposed as a powerful method that enables continuous monitoring of biochemical changes in the process of the differentiation of stem cells. This review provides a comprehensive overview of current analytical methods employed for stem cell engineering and future perspectives of nano-biosensing technologies as a platform for the in situ monitoring of stem cell status and differentiation.

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Physicochemical understanding of biomineralization by molecular vibrational spectroscopy: From mechanism to nature

et al. 2023

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The process and mechanism of biomineralization and relevant physicochemical properties of mineral crystals are remarkably sophisticated multidisciplinary fields that include biology, chemistry, physics, and materials science. The components of the organic matter, structural construction of minerals, and related mechanical interaction, etc., could help to reveal the unique nature of the special mineralization process. Herein, the paper provides an overview of the biomineralization process from the perspective of molecular vibrational spectroscopy, including the physicochemical properties of biomineralized tissues, from physiological to applied mineralization. These physicochemical characteristics closely to the hierarchical mineralization process include biological crystal defects, chemical bonding, atomic doping, structural changes, and content changes in organic matter, along with the interface between biocrystals and organic matter as well as the specific mechanical effects for hardness and toughness. Based on those observations, the special physiological properties of mineralization for enamel and bone, as well as the possible mechanism of pathological mineralization and calcification such as atherosclerosis, tumor micro mineralization, and urolithiasis are also reviewed and discussed. Indeed, the clearly defined physicochemical properties of mineral crystals could pave the way for studies on the mechanisms and applications.

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Raman imaging as a new analytical tool for the quality control of the monitoring of osteogenic differentiation in forming 3D bone tissue

Cited by 8 publications

References 32 publications

Multimodal Imaging Based on Vibrational Spectroscopies and Mass Spectrometry Imaging Applied to Biological Tissue: A Multiscale and Multiomics Review

Multimodal Imaging Based on Vibrational Spectroscopies and Mass Spectrometry Imaging Applied to Biological Tissue: A Multiscale and Multiomics Review

Recent Advances in Monitoring Stem Cell Status and Differentiation Using Nano-Biosensing Technologies

Physicochemical understanding of biomineralization by molecular vibrational spectroscopy: From mechanism to nature

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