Raman Sensing and Its Multimodal Combination with Optoacoustics and OCT for Applications in the Life Sciences

Meinhardt‐Wollweber, Merve; Roth, Bernhard

doi:10.3390/s19102387

Cited by 20 publications

(18 citation statements)

References 147 publications

(206 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Over the last two decades, tremendous advances in both the resolution and speed of optical coherence tomography (OCT) imaging, coupled with its non-invasive nature, have led to its widespread adoption [ 20 , 21 ]. Similarly, wide-field imaging modalities, including fundus autofluorescence, are being used increasingly to aid in the identification of lipofuscin [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Detecting Progression of Melanocytic Choroidal Tumors by Sequential Imaging: Is Ultrasonography Necessary?

Roelofs

O’Day

Harby

et al. 2020

Cancers

View full text Add to dashboard Cite

Purpose: To determine if ultrasonography is necessary to detect progression of choroidal melanocytic tumors undergoing sequential multi-modal imaging with color photography, autofluorescence (AF) and optical coherence tomography (OCT). Methods: All patients with choroidal melanoma undergoing treatment at Moorfields Eye Hospital between January 2016 and March 2020 were reviewed to identify those with treatment deferred by ≥ 2 months. Tumors that showed progression prior to treatment, defined as an increase in (a) basal dimensions (b) thickness (c) orange pigment and/or (d) sub-retinal fluid, were included. Mushroom shape, Orange pigment, Large size, Enlargement and Sub-retinal fluid (MOLES) scores were assigned to all tumors at earliest date and date of treatment. Results: A total of 99 patients with a mean age of 66 years (range: 26–90) were included. The initial MOLES score was 1 in 2 cases, 2 in 23 cases, and ≥ 3 in 74 cases. Progression was detected with sequential color photography alone in 100% of MOLES 1/2 and 97% of lesions with a MOLES score of ≥ 3. When findings on AF and OCT were included, sensitivity for detecting subtle change without ultrasonography improved to 100% for MOLES 3 and 97% for MOLES 4/5. Only one patient included in this study had an isolated increase in thickness that may have been missed had sequential ultrasonography not been performed. Overall, the sensitivity for detecting progression with color photographs alone was 97% (95% CI 93–100%) and increased to 99% (95% CI 97–100%) by including autofluorescence and OCT. Conclusions: Monitoring of choroidal nevi, particularly those classified as MOLES 1 or 2 (i.e., low-risk or high-risk naevi), can be accomplished safely without the need for ultrasonography. The findings of this study may remove barriers to the implementation of tele-oncology clinics for the monitoring of choroidal melanocytic tumors.

show abstract

Section: Introductionmentioning

confidence: 99%

Detecting Progression of Melanocytic Choroidal Tumors by Sequential Imaging: Is Ultrasonography Necessary?

Roelofs

O’Day

Harby

et al. 2020

Cancers

View full text Add to dashboard Cite

show abstract

“…New insights obtained from the simulations serve as valuable input for the design of novel instruments based on non-invasive, optical principles or facilitate the interpretation of measured data obtained from biological samples. The simulation methods presented in this review are mainly focused on biomedical optical imaging and spectroscopy [67]. However, they will likely influence further fields of application in the near future.…”

Section: Discussionmentioning

confidence: 99%

Advances in the simulation of light–tissue interactions in biomedical engineering

2019

Self Cite

View full text Add to dashboard Cite

Monte Carlo (MC) simulation for light propagation in scattering and absorbing media is the gold standard for studying the interaction of light with biological tissue and has been used for years in a wide variety of cases. The interaction of photons with the medium is simulated based on its optical properties and the original approximation of the scattering phase function. Over the past decade, with the new measurement geometries and recording techniques invented also the corresponding sophisticated methods for the description of the underlying light-tissue interaction taking into account realistic parameters and settings were developed. Applications, such as multiple scattering, optogenetics, optical coherence tomography, Raman spectroscopy, polarimetry and Mueller matrix measurement have emerged and are still constantly improved. Here, we review the advances and recent applications of MC simulation for the active field of the life sciences and the medicine pointing out the new insights enabled by the theoretical concepts.

show abstract

“…Multimodal CARS systems have been established, such as CARS/SHG/2PEF imaging [141][142][143], CARS/ SHG/2PEF/THG imaging [144] and OCT imaging of murine skin [142,145], to provide multi-source and complementary information and enhanced contrast of various cellular features.…”

Section: Coherent Anti-stokes Raman Scatteringmentioning

confidence: 99%

Imaging approaches for monitoring three‐dimensional cell and tissue culture systems

Hilzenrat

Gill

McArthur

2022

Journal of Biophotonics

View full text Add to dashboard Cite

The past decade has seen an increasing demand for more complex, reproducible and physiologically relevant tissue cultures that can mimic the structural and biological features of living tissues. Monitoring the viability, development and responses of such tissues in real‐time are challenging due to the complexities of cell culture physical characteristics and the environments in which these cultures need to be maintained in. Significant developments in optics, such as optical manipulation, improved detection and data analysis, have made optical imaging a preferred choice for many three‐dimensional (3D) cell culture monitoring applications. The aim of this review is to discuss the challenges associated with imaging and monitoring 3D tissues and cell culture, and highlight topical label‐free imaging tools that enable bioengineers and biophysicists to non‐invasively characterise engineered living tissues.

show abstract

Raman Sensing and Its Multimodal Combination with Optoacoustics and OCT for Applications in the Life Sciences

Cited by 20 publications

References 147 publications

Detecting Progression of Melanocytic Choroidal Tumors by Sequential Imaging: Is Ultrasonography Necessary?

Detecting Progression of Melanocytic Choroidal Tumors by Sequential Imaging: Is Ultrasonography Necessary?

Advances in the simulation of light–tissue interactions in biomedical engineering

Imaging approaches for monitoring three‐dimensional cell and tissue culture systems

Contact Info

Product

Resources

About