Raman spectroscopy analysis of differences in composition of spent culture media of in vitro cultured preimplantation embryos isolated from normal and fat mice dams

Fabian, Denis; Kačmarová, Martina; Kubandová, Janka; Číkoš, Štefan; Koppel, Juraj

doi:10.1016/j.repbio.2016.02.002

Cited by 6 publications

(5 citation statements)

References 36 publications

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“…The results of previous studies evaluating the relationship between obesity and reproduction have suggested that maternal overweight status might affect the metabolic activity of preimplantation embryos ( 42 , 43 , 47 ). To support this hypothesis, we compared the expression profiles of two genes associated with fat metabolism: perilipin 2 and fatty acid transporter protein 4.…”

Section: Discussionmentioning

confidence: 99%

The Responses of Mouse Preimplantation Embryos to Leptin In Vitro in a Transgenerational Model for Obesity

et al. 2017

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The aim of the present study was to test the hypothesis that leptin can directly mediate the negative effect of maternal obesity on preimplantation embryos. As previously shown, maternal obesity retards early embryonic development in vivo and increases the incidence of apoptosis in blastocysts. When two-cell embryos isolated from control and obese mice were transferred to identical (leptin free) conditions in vitro, no differences in any growth or quality parameters were recorded, including apoptosis incidence in blastocysts. Embryos isolated from control mice responded to transfer to environments with a high concentration of leptin (10 ng/mL) with a significant increase in arrest at the first or subsequent cell cycle. However, the majority of non-arrested embryos developed into blastocysts, showing morphology comparable to those cultured in the leptin-free group. On the other hand, the exposure of embryos isolated from obese mice to high leptin concentration in vitro did not retard their development. Furthermore, these embryos developed into blastocysts, showing a lower incidence of apoptosis. In vivo-developed blastocysts recovered from obese mice showed elevated expression levels of the proapoptotic gene BAX and the insulin-responsive glucose transporter gene SLC2A4. In conclusion, elevated leptin levels have both positive and negative effects on preimplantation embryo development in vitro, a response that likely depends on the body condition of the embryo donor. Moreover, these results suggest that leptin acts as a survival factor rather than an apoptotic inductor in embryonic cells. Since no elevations in the expression of the leptin receptor gene (LEPR) or fat metabolism-associated genes (PLIN2, SLC27A4) were recorded in blastocysts recovered from obese mice, the role of leptin in mediating the effects of obesity on embryos at the peripheral level is likely lower than expected.

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Section: Discussionmentioning

confidence: 99%

The Responses of Mouse Preimplantation Embryos to Leptin In Vitro in a Transgenerational Model for Obesity

et al. 2017

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“…Published data on the effect of being overweight on the developmental dynamics of recovered zygotes or 2 cell embryos in vitro are controversial as well: some authors have documented no effect [20,26], whereas others have documented reduced ability of ova originating in obese animals to reach higher stages of development [8]. In humans, similar contradictory findings have been reported [43,53].…”

Section: Developmental Ratementioning

confidence: 98%

“…Mated dams from both groups were sacrificed using cervical dislocation and were subjected to ova/embryo isolation on embryonic day 2 of pregnancy (ED2, approx. 32 h after presumed ovulation [15,20]), on embryonic day 3 of pregnancy (ED3, approx. 56 h after presumed ovulation [16]), and on day 4 of pregnancy (ED4, approx.…”

Section: Recovery Of Ova and Embryosmentioning

confidence: 99%

“…As shown in our previous studies, blastocysts recovered from obese mice displayed significantly elevated incidence of apoptotic cell death [11], altered gene expression (higher BAX/BCL2L2 ratio, higher expression of insulin receptor [16], and higher expression of GLUT4, an insulin-responsive glucose transporter [15]), and development of insulin resistance [16]. Furthermore, the origin of embryos (from obese vs. control dams) significantly altered their overall metabolic activity in vitro, i.e., their metabolomic profile assessed by means of Raman spectroscopy [20], their response to leptin and insulin during development in vitro [15,16], and their sensitivity to oxidative stress during development in vitro [37]. Similarly, in human in vitro developed blastocysts, fewer cells in the trophectoderm and poor glucose uptake were demonstrated in a group of obese women [43].…”

Section: Developmental Ratementioning

confidence: 99%

“…So far, the intergenerational model of diet-induced obesity has been used to evaluate the effect of female overweight status on various qualitative parameters of ovarian cells, oocytes, and early embryos, such us lipid deposition, metabolic activity, hormonal activity, apoptosis incidence, gene expression, and DNA methylation [11,[14][15][16][19][20][21][22]. The results have helped to clarify some of the unknown aspects of this relationship.…”

Section: Introductionmentioning

confidence: 99%

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Overweight and Fertility: What We Can Learn from an Intergenerational Mouse Obesity Model

Fabian

Kubandová-Babeľová

Kšiňanová

et al. 2022

IJERPH

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The aim of this study was to evaluate the effects of being overweight on the ability to conceive, fertilization rate, and in vivo development of embryos in regularly cycling, spontaneously ovulating, and naturally mated female mice. The study was based on statistical analysis of data collected during 14 experiments with identical design, performed on 319 control and 327 obese mice, developed in an intergenerational model of obesity induction which eliminates the impact of aging and high-fat feeding. Six-week-old mice with a vaginal sperm plug were slaughtered on embryonic days 2, 3, or 4, and the flushed contents of the oviducts and uteri were assessed by stereomicroscopy. The results showed no association between being overweight and the proportion of ovulating or fertilized females. On the other hand, a strong association was found between being overweight and ovulation yield. On embryonic day 2, significantly higher numbers of eggs were recovered from the oviducts of fertilized obese mice. Maternal overweight status was also associated with higher developmental capacities of preimplantation embryos. In conclusion, contrary to studies based on the high-fat-diet model, in female mice fed regular chow, being overweight was associated with an increased ovulation quota and higher developmental rate of fertilized oocytes. Being overweight did not impact ability to conceive. On the other hand, as documented in our previous studies, the quality of oocytes and blastocysts recovered from overweight mice developed in an intergenerational model of obesity was low.

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Non-invasive metabolomic profiling of culture media of ICSI- and IVF-derived early developmental cattle embryos via Raman spectroscopy

Cao

Han

et al. 2018

Animal Reproduction Science

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Raman spectroscopy analysis of differences in composition of spent culture media of in vitro cultured preimplantation embryos isolated from normal and fat mice dams

Cited by 6 publications

References 36 publications

The Responses of Mouse Preimplantation Embryos to Leptin In Vitro in a Transgenerational Model for Obesity

The Responses of Mouse Preimplantation Embryos to Leptin In Vitro in a Transgenerational Model for Obesity

Overweight and Fertility: What We Can Learn from an Intergenerational Mouse Obesity Model

Non-invasive metabolomic profiling of culture media of ICSI- and IVF-derived early developmental cattle embryos via Raman spectroscopy

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