Raman spectroscopy–based insight into lipid droplets presence and contents in liver sinusoidal endothelial cells and hepatocytes

Szafraniec, Ewelina; Kuś, Edyta; Wislocka, Adrianna; Kukla, Bozena; Sierka, Ewa; Untereiner, Valérie; Sockalingum, Ganesh D.; Chłopicki, Stefan; Barańska, Małgorzata

doi:10.1002/jbio.201800290

Cited by 27 publications

(19 citation statements)

References 62 publications

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“…6, antimycin A). Similar to another report where Raman spectroscopy was used to investigate LSECs (Szafraniec et al 2019), we distinguished numerous lipid droplets around cell nuclei (Fig. 6, arrowheads).…”

Section: -D Atomic Force Spectroscopy: Elasticity As a Parameter Dessupporting

confidence: 83%

Biophysical nanocharacterization of liver sinusoidal endothelial cells through atomic force microscopy

et al. 2020

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The structural-functional hallmark of the liver sinusoidal endothelium is the presence of fenestrae grouped in sieve plates. Fenestrae are open membrane bound pores supported by a (sub)membranous cytoskeletal lattice. Changes in number and diameter of fenestrae alter bidirectional transport between the sinusoidal blood and the hepatocytes. Their physiological relevance has been shown in different liver disease models. Although the structural organization of fenestrae has been well documented using different electron microscopy approaches, the dynamic nature of those pores remained an enigma until the recent developments in the research field of four dimensional (4-D) AFM. In this contribution we highlight how AFM as a biophysical nanocharacterization tool enhanced our understanding in the dynamic behaviour of liver sinusoidal endothelial fenestrae. Different AFM probing approaches, including spectroscopy, enabled mapping of topography and nanomechanical properties at unprecedented resolution under live cell imaging conditions. This dynamic biophysical characterization approach provided us with novel information on the 'short' lifespan , formation, disappearance and closure of hepatic fenestrae. These observations are briefly reviewed against the existing literature.

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Section: -D Atomic Force Spectroscopy: Elasticity As a Parameter Dessupporting

confidence: 83%

Biophysical nanocharacterization of liver sinusoidal endothelial cells through atomic force microscopy

et al. 2020

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“…KMC analysis was performed in order to segregate pixel Raman spectra into two main subcellular compartments, i.e., cytoplasm and nuclei ( Figure 3 B). Nuclei were identified by the DNA marker bands at 790 (breathing vibrations of thymine and cytosine with O-P-O backbone mode), 1093 (symmetric stretching vibration of the PO 2 group), 1261/1311/1344 (CH deformations of T, A and G), and 1483/1582 cm −1 (stretches of the G and A rings) [ 18 , 45 , 46 ].…”

Section: Resultsmentioning

confidence: 99%

Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy

Dorosz

Grosicki

Dybaś

et al. 2020

Cells

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Leukocytes are a part of the immune system that plays an important role in the host’s defense against viral, bacterial, and fungal infections. Among the human leukocytes, two granulocytes, neutrophils (Ne) and eosinophils (EOS) play an important role in the innate immune system. For that purpose, eosinophils and neutrophils contain specific granules containing protoporphyrin-type proteins such as eosinophil peroxidase (EPO) and myeloperoxidase (MPO), respectively, which contribute directly to their anti-infection activity. Since both proteins are structurally and functionally different, they could potentially be a marker of both cells’ types. To prove this hypothesis, UV−Vis absorption spectroscopy and Raman imaging were applied to analyze EPO and MPO and their content in leukocytes isolated from the whole blood. Moreover, leukocytes can contain lipidic structures, called lipid bodies (LBs), which are linked to the regulation of immune responses and are considered to be a marker of cell inflammation. In this work, we showed how to determine the number of LBs in two types of granulocytes, EOS and Ne, using fluorescence and coherent anti-Stokes Raman scattering (CARS) microscopy. Spectroscopic differences of EPO and MPO can be used to identify these cells in blood samples, while the detection of LBs can indicate the cell inflammation process.

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“…Fourier transform infrared (FT-IR) and Raman spectroscopies are efficient techniques in providing direct and detailed data about the molecular and chemical composition of biological specimens [ 45 , 46 , 47 ]. They are very helpful in understanding and explaining biological processes, especially at the molecular level [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Gelation Temperature on the Molecular Structure and Physicochemical Properties of the Curdlan Matrix: Spectroscopic and Microscopic Analyses

Gieroba

Sroka-Bartnicka

Kazimierczak

et al. 2020

IJMS

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In order to determine the effect of different gelation temperatures (80 °C and 90 °C) on the structural arrangements in 1,3-β-d-glucan (curdlan) matrices, spectroscopic and microscopic approaches were chosen. Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy are well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes. X-ray photoelectron spectroscopy (XPS) is a quantitative analytical method utilized in the surface study, which provided information about the elemental and chemical composition with high surface sensitivity. Contact angle goniometer was applied to evaluate surface wettability and surface free energy of the matrices. In turn, the surface topography characterization was obtained with the use of atomic force microscopy (AFM) and scanning electron microscopy (SEM). Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration. This study shows that the 1,3-β-d-glucan polymer sample gelled at 80 °C has a distinctly different structure than the matrix gelled at 90 °C.

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Raman spectroscopy–based insight into lipid droplets presence and contents in liver sinusoidal endothelial cells and hepatocytes

Cited by 27 publications

References 62 publications

Biophysical nanocharacterization of liver sinusoidal endothelial cells through atomic force microscopy

Biophysical nanocharacterization of liver sinusoidal endothelial cells through atomic force microscopy

Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy

Effect of Gelation Temperature on the Molecular Structure and Physicochemical Properties of the Curdlan Matrix: Spectroscopic and Microscopic Analyses

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