Raman spectroscopy for the analysis of different exo-polysaccharides produced by bacteria

Tahir, Muhammad; Majeed, Muhammad Irfan; Nawaz, Haq; Ali, Saqib; Rashid, Nosheen; Kashif, Mohammad; Ashfaq, Iram; Ahmad, Waqar; Ghauri, Komal; Sattar, Fazal; Jawad, Iqra; Ghauri, Muhammad Afzal; Anwar, Munir A.

doi:10.1016/j.saa.2020.118408

Cited by 20 publications

(5 citation statements)

References 23 publications

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“…In addition, the detection of genes with domains for glycosyltransferases was reported in this work for the first time for Fructobacillus spp. Although Endo et al [ 38 ] failed to detect EPS production in some Fructobacillus type strains, Tahir et al [ 75 ] found two EPS-producer F . fructosus strains able to synthesize levan and dextran.…”

Section: Discussionmentioning

confidence: 99%

Genomic diversity in Fructobacillus spp. isolated from fructose-rich niches

et al. 2023

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The Fructobacillus genus is a group of obligately fructophilic lactic acid bacteria (FLAB) that requires the use of fructose or another electron acceptor for their growth. In this work, we performed a comparative genomic analysis within the genus Fructobacillus by using 24 available genomes to evaluate genomic and metabolic differences among these organisms. In the genome of these strains, which varies between 1.15- and 1.75-Mbp, nineteen intact prophage regions, and seven complete CRISPR-Cas type II systems were found. Phylogenetic analyses located the studied genomes in two different clades. A pangenome analysis and a functional classification of their genes revealed that genomes of the first clade presented fewer genes involved in the synthesis of amino acids and other nitrogen compounds. Moreover, the presence of genes strictly related to the use of fructose and electron acceptors was variable within the genus, although these variations were not always related to the phylogeny.

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Section: Discussionmentioning

confidence: 99%

Genomic diversity in Fructobacillus spp. isolated from fructose-rich niches

et al. 2023

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“…Based on optimized conditions, the isolate HBW1 was incubated in MRS medium including 250 g sucrose in a shaking incubator (150 rpm) for 72 h at 30 °C. The EPS was purified according to previous methods [ 77 ].…”

Section: Methodsmentioning

confidence: 99%

High-Molecular-Weight Dextran-Type Exopolysaccharide Produced by the Novel Apilactobacillus waqarii Improves Metabolic Syndrome: In Vitro and In Vivo Analyses

Ahmad

Boyajian

Abosalha

et al. 2022

IJMS

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Metabolic syndrome is a leading medical concern that affects one billion people worldwide. Metabolic syndrome is defined by a clustering of risk factors that predispose an individual to cardiovascular disease, diabetes and stroke. In recent years, the apparent role of the gut microbiota in metabolic syndrome has drawn attention to microbiome-engineered therapeutics. Specifically, lactic acid bacteria (LAB) harbors beneficial metabolic characteristics, including the production of exopolysaccharides and other microbial byproducts. We recently isolated a novel fructophilic lactic acid bacterium (FLAB), Apilactobacillus waqarii strain HBW1, from honeybee gut and found it produces a dextran-type exopolysaccharide (EPS). The objective of this study was to explore the therapeutic potential of the new dextran in relation to metabolic syndrome. Findings revealed the dextran’s ability to improve the viability of damaged HT-29 intestinal epithelial cells and exhibit antioxidant properties. In vivo analyses demonstrated reductions in body weight gain and serum cholesterol levels in mice supplemented with the dextran, compared to control (5% and 17.2%, respectively). Additionally, blood glucose levels decreased by 16.26% following dextran supplementation, while increasing by 15.2% in non-treated mice. Overall, this study displays biotherapeutic potential of a novel EPS to improve metabolic syndrome and its individual components, warranting further investigation.

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“…The surface-enhanced Raman spectroscopy of PEP-1-1 is shown in Figure 2; significant polysaccharide signal peaks were observed within it, including C-C-O-C-O stretching vibration at 853 cm −1 [33], C-H stretching vibration at 925 cm −1 [34], C-O shear vibration at 1120 cm −1 [35], C-O-H bending vibration at 1300 cm −1 [36], C-H bending with some O-H contribution at 1410 cm −1 , and CH 2 stretching vibration at 1455 cm −1 were [37].…”

Section: Surface-enhanced Raman Spectroscopy Analysismentioning

confidence: 99%

Exploration of Polysaccharides from Phyllanthus emblica: Isolation, Identification, and Evaluation of Antioxidant and Anti-Glycolipid Metabolism Disorder Activities

Guo,

Chen,

Wang

et al. 2024

Molecules

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Phyllanthus emblica is a natural medicinal herb with diverse bioactivities. Certain extracts from this herb have been confirmed to possess anti-glycolipid metabolic disorder activity. To further develop its utility value and explore its potential in combating glycolipid metabolic disorders, we designed a series of experiments to investigate the structure, antioxidant activity, and anti-glycolipid metabolic disorder activity of Phyllanthus emblica polysaccharides. In this study, we extracted and purified polysaccharides from Phyllanthus emblica and thoroughly analyzed their structure using various techniques, including NMR, methylation analysis, and surface-enhanced Raman spectroscopy. We investigated the hypolipidemic and anti-glycolipid metabolism disorder activity of Phyllanthus emblica polysaccharides for the first time utilizing oleic acid (OA) and advanced glycation end products (AGEs) as inducers. Additionally, the antioxidant activity of Phyllanthus emblica polysaccharides was assessed in vitro. These findings lay the groundwork for future investigations into the potential application of Phyllanthus emblica polysaccharides as an intervention for preventing and treating diabetes.

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Raman spectroscopy for the analysis of different exo-polysaccharides produced by bacteria

Cited by 20 publications

References 23 publications

Genomic diversity in Fructobacillus spp. isolated from fructose-rich niches

Genomic diversity in Fructobacillus spp. isolated from fructose-rich niches

High-Molecular-Weight Dextran-Type Exopolysaccharide Produced by the Novel Apilactobacillus waqarii Improves Metabolic Syndrome: In Vitro and In Vivo Analyses

Exploration of Polysaccharides from Phyllanthus emblica: Isolation, Identification, and Evaluation of Antioxidant and Anti-Glycolipid Metabolism Disorder Activities

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