Randomized comparison of virtual microscopy and traditional glass microscopy in diagnostic accuracy among dermatology and pathology residents

Koch, Laine H.; Lampros, James N.; DeLong, Laura K.; Chen, Shuo; Woosley, John T.; Hood, Antoinette F.

doi:10.1016/j.humpath.2008.10.009

Cited by 91 publications

(124 citation statements)

References 14 publications

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“…It has been shown that the diagnostic accuracy using digital slides is similar to conventional glass slides [11]. It would not have been possible to circulate glass slides to 15 experts in 11 countries within a reasonable time frame.…”

Section: Discussionmentioning

confidence: 99%

Interactive digital slides with heat maps: a novel method to improve the reproducibility of Gleason grading

et al. 2011

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Our aims were to analyze reporting of Gleason pattern (GP) 3 and 4 prostate cancer with the ISUP 2005 Gleason grading and to collect consensus cases for standardization. We scanned 25 prostate biopsy cores diagnosed as Gleason score (GS) 6-7. Fifteen genitourinary pathologists graded the digital slides and circled GP 4 and 5 in a slide viewer. Grading difficulty was scored as 1-3. GP 4 components were classified as Type 1 (cribriform), 2 (fused) or 3 (poorly formed glands). A GS 5-6, 7 (3+4), 7 (4+3), 8-9 was given in 29%, 41%, 19% and 10% (mean GS 6.84,). In 15 cases, at least 67% of observers agreed on GS groups (consensus cases). Mean interobserver weighted kappa for GS groups was 0.43.Mean difficulty scores in consensus and non-consensus cases were 1.44 and 1.66 (p = 0.003).Pattern 4 Types 1, 2 and 3 were seen in 28%, 86% and 67% of GP 4. All three co-existed in 16% (11% and 23% in consensus and non-consensus cases, p = 0.03). Average estimated and calculated %GP 4/5 were 29% and 16%. After individual review, the experts met to analyze diagnostic difficulties. Areas of GP 4 and 5 were displayed as heat maps, which were helpful for identifying contentious areas. A key problem was to agree on minimal criteria for small foci of GP 4. In summary, the detection threshold for GP 4 in NBX needs to be better defined.This set of consensus cases may be useful for standardization.

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Section: Discussionmentioning

confidence: 99%

Interactive digital slides with heat maps: a novel method to improve the reproducibility of Gleason grading

et al. 2011

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“…Web-based virtual microscopy provides a platform similar to the optical microscope to study tissues, allowing movement of the ''slide'' in order to examine multiple sections of the tissue and allows for changes in magnification. Virtual microscopy has been adapted to several higher-level professional programs, such as medical (Blake et al, 2003;Krippendorf and Lough, 2005;Helle et al, 2011), dental (Szymas and Lundin, 2011), graduate residency programs (Bruch et al, 2009;Koch et al, 2009) and veterinary schools (Mills et al, 2007), and specifically to courses like medical histology (Scoville and Buskirk, 2007;Schmidt et al, 2011), pathology (Dee and Meyerholz, 2007;Braun and Kearns, 2008;Cunningham et al, 2008;Kim et al, 2008), dental histology (Weaker and Herbert, 2009), and veterinary cytopathology (Neel et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Optical versus virtual: Teaching assistant perceptions of the use of virtual microscopy in an undergraduate human anatomy course

Collier

Dunham

Braun

et al. 2011

Anatomical Sciences Ed

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Many studies that evaluate the introduction of technology in the classroom focus on student performance and student evaluations. This study focuses on instructor evaluation of the introduction of virtual microscopy into an undergraduate anatomy class. Semi-structured interviews were conducted with graduate teaching assistants (TA) and analyzed through qualitative methods. This analysis showed that the teaching assistants found the virtual microscope to be an advantageous change in the classroom. They cite the ease of use of the virtual microscope, access to histology outside of designated laboratory time, and increasing student collaboration in class as the primary advantages. The teaching assistants also discuss principal areas where the use of the virtual microscope can be improved from a pedagogical standpoint, including requiring students to spend more time working on histology in class.

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“…Digital pathology, and specifically WSI, has been previously implemented around the world for education [7] and research [8] . Recently, its adoption for diagnostic purposes has been increasing.…”

Section: Discussionmentioning

confidence: 99%

Diagnosing Pathological Prognostic Factors in Retinoblastoma: Correlation between Traditional Microscopy and Digital Slides

et al. 2015

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Objective: It was the aim of this study to determine the diagnostic accuracy of high-risk prognostic factors and morphological characteristics of retinoblastomas using digital whole slide images (WSI) generated by a scanner. Methods: Forty-seven H&E sections of glass slides with high-risk morphological features of retinoblastoma were analyzed. Slides were scanned as WSI and reviewed. The results were compared with those obtained after reviewing the slides using a regular microscope as the gold standard. McNemar's test (MT), the percentage of agreement (POA), and sensitivity (S) and specificity (Sp) were evaluated between WSI and conventional microscopy. Results: There were no differences with respect to multicentricity, growth type, rosette formation, choroidal invasion, anterior chamber invasion, extraocular extension, scleral extension, optic nerve invasion, necrosis, or Azzopardi effect between WSI analysis and light microscopy (MT, p = 1.0; POA = 100%; S = 100%, and Sp = 100%). Discordance was found in 1 case where calcification could not be found using WSI (MT, p = 1.00; POA = 97.9%; S = 100%, and Sp = 97.8%). Conclusion: To the best of our knowledge, this is the first report using digital pathology (WSI) to evaluate prognostic factors in eyes containing retinoblastomas. Using WSI, the pathologist was able to detect highrisk morphological features in retinoblastoma. To date, WSI is an important tool, in particular for ophthalmic pathologists examining enucleation and exenteration specimens.

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Randomized comparison of virtual microscopy and traditional glass microscopy in diagnostic accuracy among dermatology and pathology residents

Cited by 91 publications

References 14 publications

Interactive digital slides with heat maps: a novel method to improve the reproducibility of Gleason grading

Interactive digital slides with heat maps: a novel method to improve the reproducibility of Gleason grading

Optical versus virtual: Teaching assistant perceptions of the use of virtual microscopy in an undergraduate human anatomy course

Diagnosing Pathological Prognostic Factors in Retinoblastoma: Correlation between Traditional Microscopy and Digital Slides

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