Randomly Amplified Polymorphic Dna-a Brief Review

Kumari, Neeraj; Thakur, Saroj Kumar

doi:10.3844/ajavsp.2014.6.13

Cited by 74 publications

(56 citation statements)

References 28 publications

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“…Phylogenetic Grouping Random amplified polymorphic DNA (RAPD) [16][17][18] and enterobacterial repetitive intergenic consensus (ERIC)-PCR [19][20][21] were performed to understand the clonal relatedness of the study isolates. Genomic DNA was recovered using NucleoSpin ® tissue kits (Macherey-Nagel).…”

Section: Phenotypic Detection Of Carbapenemase Expressionmentioning

confidence: 99%

Detection of Antibiotic Resistance Determinants and Their Transmissibility among Clinically Isolated Carbapenem-Resistant Escherichia coli from South India

et al. 2018

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Objectives: The aim of this study was to analyze the prevalence of the CTX-M, TEM, SHV, VIM, NDM, and OXA genes in carbapenemase-producing Escherichia coli and their transmissibility at a tertiary care hospital in south India. Materials and Methods: Twenty-one carbapenem-resistant E. coli (carbapenem-resistant Enterobacteriaceae; CRE) were collected from the Sri Sathya Sai Institute of Higher Medical Sciences (Puttaparthi India). Resistance to antibiotics was analyzed by Vitek-2, and the identity of the isolates was confirmed by 16S rDNA sequencing. RAPD and enterobacterial repetitive intergenic consensus (ERIC)-PCR were performed for molecular typing. Metallo-β-lactamase production was confirmed by a double disc synergy test. The presence of the extended-spectrum β-lactamases CTX-M, TEM, and SHV and of the carbapenemases NDM, VIM, and OXA was determined by PCR. Carbapenemase variants were further confirmed by sequencing. The transmissibility of the genes was tested by conjugation. Results: Twelve of the 21 (57%) carbapenem-resistant E. coli isolates were community acquired, indicating the spread of CRE in environmental samples. TEM and NDM-5 were found to be the major β-lactamases produced by the pathogens. OXA-181 was found in 5 of the isolates. All 21 isolates were found to harbor more than one of the tested β-lactamases, and all of the isolates were found to have the capacity to participate in conjugation; 15 of the transconjugants were found to have acquired the tested β-lactamases, substantiating their ability to be transferred to other strains of bacteria. Conclusion: Monitoring of community-acquired carbapenem-resistant bacteria is very important as the association of resistance determinants with mobile genetic elements would present a serious clinical challenge.

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Section: Phenotypic Detection Of Carbapenemase Expressionmentioning

confidence: 99%

Detection of Antibiotic Resistance Determinants and Their Transmissibility among Clinically Isolated Carbapenem-Resistant Escherichia coli from South India

et al. 2018

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“…Additionally, RAPD are not locus-specific, so electrophoretic band profiles can not be interpreted in terms of loci and alleles and fragments of similar size may not be homologous (Martínez et al, 2010;Kumari and Thakur, 2014).…”

Section: Resultados Y Discusiónmentioning

confidence: 99%

Identificación de híbridos de Citrus aurantifolia X Citrus limon utilizando marcadores de Secuencias Simples Repetidas (SSR)

Guzmán¹,

Rodríguez²,

Mariscal³

et al. 2017

Remexca

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ResumenLa apomixis es un tipo de reproducción asexual donde la formación de semillas porta embriones genéticamente idénticos al progenitor, constituyendo un obstáculo en programas de mejoramiento genético de muchas especies vegetales, incluyendo cítricos. La identificación de plantas híbridas se realiza mediante caracteres morfológicos, ensayos isoenzimáticos y marcadores moleculares. Estos últimos se han utilizado con mayor frecuencia debido a su precisión, destacando el uso del DNA polimórfico amplificado al azar (RAPD, "Random Amplified Polymorphic DNA") y Secuencias Simples Repetidas (SSR, Simple Sequence Repeats). En limón mexicano (C. aurantifolia) únicamente se han utilizado marcadores RAPD para la identificación de híbridos, por lo que no existen reportes que hagan uso de marcadores SSR para este fin. El objetivo del presente trabajo fue identificar híbridos derivados de la polinización controlada entre C. aurantifolia var. "Colimex"×C. limon var. "Rosenberg" y su recíproca utilizando marcadores moleculares SSR. Durante el año 2014-2016 se colectaron hojas de árboles de limón de aproximadamente 12 meses de edad, que se encuentran establecidos en el Campo AbstractApomixis is a type of asexual reproduction where the seeds formation carries genetically identical embryos to the parent, constituting an obstacle in breeding programs for many plant species, including citrus. The identification of hybrid plants is carried out by morphological characters, isoenzymatic assays and molecular markers. The latter have been used more frequently because of their accuracy, standind out the use of random amplified polymorphic DNA (RAPD) and Simple Sequence Repeats (SSR). In Mexican lime (C. aurantifolia) RAPD markers have only been used for hybrid identification, so there are no reports that use SSR markers for this purpose. The aim of this paper was to identify hybrids derived from controlled pollination between C. aurantifolia var. "Colimex" × C. limon var. "Rosenberg" and its reciprocal using SSR molecular markers. During the 2014-2016 years, leaves of lime trees of approximately 12 months of age were collected, which are established in the Campo Experimental Tecomán of the INIFAP. A total of eight SSR molecular markers were evaluated on the progenitors used in this study and the TAA45 and cAGG09 oligonucleotides were selected for the hybrids identification in the two progeny1398 Rev. Mex. Cienc. Agríc. Vol.8 Núm. 6 14 de agosto -26 de septiembre, 2017 Manuel de Jesús Bermúdez Guzmán et al.Experimental Tecomán del INIFAP. Se evaluaron en total ocho marcadores moleculares SSR sobre los progenitores utilizados en este estudio y fueron seleccionados los oligonucleótidos TAA45 y cAGG09 para la identificación de híbridos en las dos poblaciones progenie. De un total de 40 y 43 individuos F1 procedentes de la cruza bidireccional entre "Colimex" × "Rosenberg", se lograron identificar 17 y 35 plantas híbridas, respectivamente. Los resultados indican que los marcadores SSR son eficientes y confiables para la identificación de hí...

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“…Each method has both advantages and disadvantages. RAPD‐PCR is one of the most widely used methods for genotyping, and it involves the use of single arbitrary short primers resulting in the amplification of many discrete DNAs (Kumari & Thakur ). Miyata et al .…”

Section: Discussionmentioning

confidence: 99%

Development and validation of glycoprotein‐based native‐subunit vaccine for fish against Aeromonas hydrophila

Çiftci

Onuk

Çiftçi

et al. 2016

Journal of Fish Diseases

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Aeromonas hydrophila is known to be causative agent of an infection named as Bacterial haemorrhagic septicaemia or red pest in freshwater fish. The aim of this study was to develop and validate the glycoprotein-based fish vaccine against Aeromonas hydrophila. For this aim, after identification and characterization of A. hydrophila isolates from fish farms, one A. hydrophila isolate was selected as vaccine strain. Antigenic glycoproteins of this vaccine strain were determined by Western blotting and glycan detection kit. The connection types of these glycoproteins were examined by glycoprotein differentiation kit. Two glycoproteins, molecular weights of 19 and 38 kDa, with SNA connection type were selected for use in vaccination trials. After their purification by SNA-specific lectin and size-exclusion chromatography, protection studies with purified proteins were performed. For challenge trials, four experimental fish groups were designated: Group I (with montanide), Group II (with montanide and ginseng), Group III [with Al(OH)3 ] and Group IV [with Al(OH)3 and ginseng]. The survival ratings of fish were determined, and protection was calculated as 21.56%, 29.41%, 69.83% and 78.88% in groups I, II, III and IV, respectively. In conclusion, A. hydrophila glycoproteins with Al(OH)3 and ginseng could be used as a safe and effective vaccine for fish.

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Randomly Amplified Polymorphic Dna-a Brief Review

Cited by 74 publications

References 28 publications

Detection of Antibiotic Resistance Determinants and Their Transmissibility among Clinically Isolated Carbapenem-Resistant <b><i>Escherichia coli</i></b> from South India

Detection of Antibiotic Resistance Determinants and Their Transmissibility among Clinically Isolated Carbapenem-Resistant <b><i>Escherichia coli</i></b> from South India

Identificación de híbridos de Citrus aurantifolia X Citrus limon utilizando marcadores de Secuencias Simples Repetidas (SSR)

Development and validation of glycoprotein‐based native‐subunit vaccine for fish against Aeromonas hydrophila

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